Anti-contamination primers to improve specificity of polymerase chain reaction in human papillomavirus screening

Beyer-Finkler, Elke; Pfister, Herbert; Girardi, Frank

doi:10.1016/0140-6736(90)91365-h

Cited by 23 publications

(10 citation statements)

References 4 publications

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“…Its major asset, its sensitivity, is also its major limitation. False positive results can be a major problem, and undoubtedly account for some of the very high HPV prevalence rates in early studies that used this technique [108,109]. Most recent investigators now include appropriate negative controls, in order to properly evaluate positive results.…”

Section: Other Head and Neck Carcinomasmentioning

confidence: 99%

A possible role for human papillomaviruses in head and neck cancer

Steinberg

DiLorenzo

1996

Cancer Metast Rev

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Human papillomaviruses (HPVs) cause benign tumors in the respiratory tract. Mounting evidence suggests that they also play a role in the etiology of a subset of head and neck cancers. Carcinomas in patients with a history of recurrent respiratory papillomatosis clearly are caused by persisting HPV interacting with one of more carcinogenic agents. Verrucous carcinomas of the oral cavity, tonsillar and tongue carcinomas are strongly linked with HPVs, based on molecular epidemiologic data. Tonsillar cancer have been shown to express HPV RNA, presumed necessary to induce and maintain a carcinoma, supporting a viral etiology. This paper reviews the molecular and cellular basis for considering HPVs as causative agents of cancer, and reviews the literature that considers the possible role of HPVs in head and neck cancer.

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Section: Other Head and Neck Carcinomasmentioning

confidence: 99%

A possible role for human papillomaviruses in head and neck cancer

Steinberg

DiLorenzo

1996

Cancer Metast Rev

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“…These observations may also explain the varying prevalence rates found in cytologically normal scrapes as reported by several groups. The great variation in HPV prevalence rates, as determined by the sensitive PCR method in cytologically normal scrapes, ranged from 6% to 80% in several studies (in order of increasing frequency: van den Brule et al, , 1990bManos et al, 1990;Beyer-Finkler et al, 1990;Young et al, 1989;Tidy et al, 1989). This may also be caused by poor definition of the study groups, in which CIN history is very important.…”

Section: With a Combination Of General Primer-mediated And Typespecifmentioning

confidence: 99%

Difference in prevalence of human papillomavirus genotypes in cytomorphologically normal cervical smears is associated with a history of cervical intraepithelial neoplasia

Brule

Walboomers

Maine

et al. 1991

Intl Journal of Cancer

130

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The prevalence of human papillomavirus (HPV) genotypes was investigated by the polymerase chain reaction (PCR) method in cytologically normal and abnormal cervical scrapes obtained from asymptomatic women (n = 1,346), participating in a triennial screening program for cervical cancer, and from a gynecological outpatient population (n = 593). In the symptom-free population oncogenic HPV types 16, 18, 31 and 33 were present in 1.5% of cytologically normal scrapes, while the overall HPV prevalence rate was 3.5%. Significantly, higher HPV prevalence rates of 7% (oncogenic HPV; p less than 0.01) and 14% (all HPV; p less than 0.01), respectively, were found in cytologically normal scrapes of the gynecologic outpatient population. It appeared that in this outpatient group 78% of the smears containing HPV 16 and 18 were associated with a history of cervical pathology, i.e. cervical intraepithelial neoplasia grade I to III. In smears with mild and severe dysplasia and smears suspected of carcinoma in situ from both populations, the overall HPV prevalence was 70%, 84% and 100%, respectively. In all squamous-cell carcinomas of the cervix (n = 50) HPV was detected. Frequencies of HPV 16 and 18 increased from 41% in mild dysplasia to 94% in cervical carcinomas. Since a low prevalence of HPV was found in cytomorphologically normal cervices of women without a clinicopathological history, the findings in this study suggest that HPV detection in population-based screening programs for cervical neoplasia can be an important tool in identifying women who are at risk of developing dysplasia and cervical cancer.

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“…Despite strict adherence to these protocols contamination cannot be ruled out entirely (75 HPV plasmid clones is the selection of primers from HPV type specific sequences which flank the cloning site of a recombinant H P V plasmid (76,77). With these primers only a HPV type specific amplification was obtained from naturally occurring HPV genomes.…”

Section: Hpv Primer Selection For the Pcrmentioning

confidence: 99%

“…With these socalled anti-contamination primers a strict differentiation could be made between truely HPV positive specimens and specimens which are contaminated with HPV plasmid clones (76,77). In a study by Beyer-Finkler et al (75), primers directed against the non-coding region (NCR)/E6 region of HPV 16 were used and 23 of 53 samples were found to be positive for HPV 16. After rescreening of these samples with HPV 16 type specific anti-contamination primers, only 13 samples remained positive.…”

Section: Hpv Primer Selection For the Pcrmentioning

confidence: 99%

Use of the polymerase chain reaction to study the relationship between human papillomavirus infections and cervical cancer

Melchers¹,

Claas

Quint

1991

Eur. J. Clin. Microbiol. Infect. Dis.

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Although it is now evident that human papillomaviruses (HPV) are strongly associated with cervical cancer, their etiological role in the oncogenesis of this disease is still unknown. However, HPV screening may identify women at risk of acquiring this disease. With the recent development of the polymerase chain reaction (PCR), it has become possible to detect small numbers of human papillomavirus genomes in clinical samples. The sensitivity and specificity of this technique, together with the possibility of performing the test on crude cervical scrapes, makes PCR the method of choice for screening. In this paper, data on the detection of human papillomavirus by PCR are presented and the applicability of this technique for the screening of human papillomavirus genotypes is evaluated. The question arises whether screening for diagnostic purposes must include all the human papillomavirus types associated with infections of the genital tract or only those which are strongly associated with cervical cancer (HPV 16 and HPV 18). It is proposed that an international council must be created that is responsible for standardised epidemiological screening strategies and follow-up programmes.

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Anti-contamination primers to improve specificity of polymerase chain reaction in human papillomavirus screening

Cited by 23 publications

References 4 publications

A possible role for human papillomaviruses in head and neck cancer

A possible role for human papillomaviruses in head and neck cancer

Difference in prevalence of human papillomavirus genotypes in cytomorphologically normal cervical smears is associated with a history of cervical intraepithelial neoplasia

Use of the polymerase chain reaction to study the relationship between human papillomavirus infections and cervical cancer

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