Anti-clastogenic Effect of Magnolol-Containing Hange-koboku-to, Dai-joki-to, Goshaku-san, and Magnoliae Cortex on Benzo(a)pyrene-Induced Clastogenicity in Mice

Abstract

Magnolol was previously shown to inhibit genotoxicity induced by environmental mutagens both in vitro and in vivo. Here, we investigate the effects of the magnolol-containing kampo (traditional) medicines Hange-kobokuto, Dai-joki-to, and Goshaku-san, as well as Magnoliae Cortex, on the clastogenesis induced by benzo(a)pyrene (B(a)P) using the mouse micronucleus test. The mice were first treated with a single intraperitoneal injection of B(a)P, followed by a single oral dose of Hange-koboku-to, Dai-joki-to, Goshaku-san, or Magnoliae Cortex. Peripheral blood specimens were prepared 48 h after B(a)P administration and analyzed using the acridine orange (AO) technique. The anti-clastogenic mechanisms employed by the kampo medicines and Magnoliae Cortex were also investigated by evaluating in vivo CYP1A1 activity using the zoxazolamine paralysis test. Results show that Hange-koboku-to, Dai-joki-to, and Magnoliae Cortex, which contain high levels of magnolol, significantly inhibited the clastogenesis induced by B(a)P and sufficiently inhibited in vivo CYP1A1 activity. In contrast, Goshakusan, which contains low levels of magnolol, had little inhibitory effect on clastogenicity and in vivo CYP1A1 activity. These findings suggest that magnolol is a major contributor to the inhibition of B(a)P-induced clastogenesis, and that kampo medicines exert significant anti-clastogenic effects.

Anti‐biofilm and bactericidal effects of magnolia bark‐derived magnolol and honokiol on Streptococcus mutans

“…Low toxicity is desirable for antimicrobial agents utilized in the mouth. Magnolia bark has low genotoxicity and even has an anticlastogenic effect in vivo , as well as having low toxicity for human epithelial cells and fibroblasts . In our study, magnolol and honokiol were both much less toxic for gingival epithelial cell lines than CHX, at least up to 1 hr after exposure.…”

Cytotoxicity and sub-acute toxicity in Crl:CD (SD) rats of traditional herbal formula Ojeok-san

“…In experimental studies, OJS has been reported to have several biochemical activities such as anti-cancer [2], anti-inflammation [3,4], anti-hyperglycemia [5], anti-clastogenic effect [6] and immune regulation [7]. In addition, Kim et al recently established OJS administration criteria and a questionnaire to evaluate its holistic effects on patients with low back pain (LBP) [8].…”

Chemistry Study on Protective Effect against·OH-induced DNA Damage and Antioxidant Mechanism of Cortex Magnoliae Officinalis

“…As mutagenic has been demonstrated to be directly associated with DNA oxidative damage, 25 thereby the anticlastogenic and antineoplastic potentials of CMO 4 are thought to arise from its protective effect against ·OH-induced DNA oxidative damage. For example, magnolol was proved to possess an inhibition of B(a)P-induced clastogenesis, B(a)Pinduced carcinogenesis however was reported to be involved in ROS.…”

“…1), a Chinese herbal medicine used in East Asia for over thousands years, presents anti-clastogenic 4 and antineoplastic potentials. 5 Therefore, we tried to use CMO as a reference to address the following problems: (i) Is the protective effect of phytophenol implicated with the other components in Chinese herbal medicine?…”