Anti-cancer vaccination by transdermal delivery of antigen peptide-loaded nanogels via iontophoresis

Toyoda, Mao; Hama, Susumu; Ikeda, Yutaka; Nagasaki, Yukio; Kogure, Kentaro

doi:10.1016/j.ijpharm.2015.02.024

Cited by 73 publications

(53 citation statements)

References 13 publications

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“…8) On the other hand, polymerbased nanoparticles accumulated in the epidermis due to their rigid structures. 7) Thus, polymer-based nanoparticles that exhibit antioxidative activity may be useful for effective prevention of UV-mediated skin injury.…”

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confidence: 90%

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Prevention of UV-Induced Melanin Production by Accumulation of Redox Nanoparticles in the Epidermal Layer <i>via</i> Iontophoresis

Shiota

Hama

Yoshitomi

et al. 2017

Biological & Pharmaceutical Bulletin

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UV rays induce melanin production in the skin, which, from a cosmetic point of view, is problematic. Reactive oxygen species (ROS) generated in the skin upon UV irradiation are thought to be responsible for melanin production. Thus, effective antioxidants are recognized as useful tools for prevention of UV-induced melanin production. Redox nanoparticles (RNPs) containing nitroxide radicals as free radical scavengers were previously developed, and shown to be effective ROS scavengers in the body. RNPs are therefore expected to be useful for effective protection against UV-induced melanin production. However, as the sizes of RNPs are typically larger than the intercellular spaces of the skin, transdermal penetration is difficult. We recently demonstrated effective transdermal delivery and accumulation of nanoparticles in the epidermal layer via faint electric treatment, i.e., iontophoresis, suggesting that iontophoresis of RNPs may be a useful strategy for prevention of UV-induced melanin production in the skin. Herein, we performed iontophoresis of RNPs on the dorsal skin of hairless mice that produce melanin in response to light exposure. RNPs accumulated in the epidermal layer upon application of iontophoresis. Further, the combination of RNPs with iontophoresis decreased UV-induced melanin spots and melanin content in the skin. Taken together, we successfully demonstrated that iontophoresis-mediated accumulation of RNPs in the epidermis prevented melanin production.

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confidence: 90%

“…7) Iontophoresis is an effective technology for transdermal delivery of nanoparticles, such as liposomes, using faint electricity. 8) We found that faint electric treatment opened intercellular junctions in the skin.…”

mentioning

confidence: 98%

Prevention of UV-Induced Melanin Production by Accumulation of Redox Nanoparticles in the Epidermal Layer <i>via</i> Iontophoresis

Shiota

Hama

Yoshitomi

et al. 2017

Biological & Pharmaceutical Bulletin

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“…Employment of silver nanoparticles or gold nanoparticles in the formulations could facilitate the passage of electric current, improving iontophoresis efficiency of OVA and signal transducer and activator of transcription 3 siRNA, respectively. Nanotechnologies such as liposomes and nanogels were also used in combination with iontophoresis to protect the macromolecular antigens from degradation.…”

Section: Advanced Technologies For Transcutaneous Gene Deliverymentioning

confidence: 99%

Transcutaneous delivery of DNA/mRNA for cancer therapeutic vaccination

Sun

Zeng

Huang

2019

The Journal of Gene Medicine

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Therapeutic vaccination is a promising strategy for the immunotherapy of cancers. It eradicates cancer cells by evoking and strengthening the patient's own immune system. Because of the easy access and sophisticated immune networks, the skin becomes an ideal target organ for vaccination. Genetic vaccines have been widely investigated, with the advantages of the delivery of multiple antigens and a lower cost for production compared to protein/peptide vaccines. This review summarizes the advances made with respect to the transcutaneous delivery of DNA/mRNA for cancer therapeutic vaccination and also gives a brief description of the immunological milieu of the skin and the importance of dendritic cell‐targeting in vaccine delivery, as well as the technologies that aim to facilitate antigen delivery and modulate antigen‐presenting cells, thus improving cellular responses. The applications of genetic vaccines encoding tumor antigens delivered through the skin route, both in preclinical and clinical trials, are outlined.

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“…Liposomes being versatile amphiphilic carriers have long been used in this regard. It has been used in oral drug delivery [44][45][46], transdermal [47][48][49][50], intravenous delivery [50] and in many other applications [51][52][53].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Phospholipid Nanoparticle Formulations Using Response Surface Methodology

Naeem

Kiew

Chung

et al. 2015

J Surfact & Detergents

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The present study deals with the optimization of phospholipid liposome formulations to mimic red blood cells. Optimization of different concentrations of distearylphosphatidylcholine, dipalmitoylphosphatidylcholine, and phosphatidylserine at a fixed concentration of lecithin and Tween® 80 was done using response surface methodology. The optimized formulation produced liposomes with a particle size in the range of 112–196 nm. The optimized formulation shows low encapsulation efficiency at low levels of insulin but increases at higher loading levels. Formulated vesicles fulfill the size requirement for intravenous drug delivery. The present system is environmentally friendly with respect to biodegradability and biocompatibility.

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Anti-cancer vaccination by transdermal delivery of antigen peptide-loaded nanogels via iontophoresis

Cited by 73 publications

References 13 publications

Prevention of UV-Induced Melanin Production by Accumulation of Redox Nanoparticles in the Epidermal Layer <i>via</i> Iontophoresis

Prevention of UV-Induced Melanin Production by Accumulation of Redox Nanoparticles in the Epidermal Layer <i>via</i> Iontophoresis

Transcutaneous delivery of DNA/mRNA for cancer therapeutic vaccination

Optimization of Phospholipid Nanoparticle Formulations Using Response Surface Methodology

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