Anti-cancer effect of <i>Petasites hybridus</i> L. (Butterbur) root extract on breast cancer cell lines

Tzoneva, Rumiana; Uzunova, Veselina; Stoyanova, Tihomira; Borisova, Borislava; Momchilova, Albena; Pankov, Roumen; Maslenkova, Liliana

doi:10.1080/13102818.2021.1932594

Cited by 7 publications

(4 citation statements)

References 23 publications

(28 reference statements)

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“…Previous studies using the DAPI staining assay have similarly shown that extracts from Petasites hybridus, Artemisia nilagirica, and Zanthoxylum armatum also initiate apoptosis in these cells, thereby exhibiting anticancer activity. [24][25][26] Consequently, these findings are compatible with the results of previously described experiments suggesting that the I. verum methanolic extract is efficient against MDA-MB-231 cancer cells.…”

Section: Discussionsupporting

confidence: 92%

Illicium verum anticancer activity against MDA-MB-231 cell line

Pahore,

Khan,

Karim

2023

Pak J Med Sci

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Objective: To evaluate the anticancer activity of methanolic extract of Illicium verum against triple-negative breast cancer MDA-MB-231 cell line. Methods: A cell culture experimental study was carried out at Pharmacology department of Bahria University Medical and Dental College (January to June 2021) in collaboration with Aga Khan University, Karachi, Pakistan. Cell viability and proliferation assays were used to quantify dead and alive cells by utilizing a tetrazolium assay and an enzyme immunosorbent plate reader was used to calculate their absorbance. For the apoptosis initiation assay, these cells were dyed with a fluorescent stain and observed for fluorescence and apoptosis. During cell viability testing, various I. verum methanolic extract doses (0.125, 0.25, 0.5, 1, 3, 6, 12, and 25µg/ml) were employed to treat MDA-MB-231 cells, while the IC50 dose of 2.8µg/ml was used for both the cell proliferation and apoptosis initiation assays. Results: In the cell viability assay, all I. verum methanolic extract doses exhibited a substantial decrease in the viability of MDA-MB-231 cells (less than 0.01 p-value). In cell proliferation assay and apoptosis initiation, the IC50 dose of 2.8µg/ml of I. verum methanolic extract also exhibited a substantial decrease in cell division (less than 0.01 p-value) and the initiation of apoptosis in MDA-MB-231 cells. Conclusion: Illicium verum methanolic extract have strong anticancer activity against triple-negative breast cancer MDA-MB-231 cell line through cytotoxicity, proliferation reduction, and apoptosis initiation mechanisms. doi: https://doi.org/10.12669/pjms.40.1.7860 How to cite this: Pahore AK, Khan S, Karim N. Illicium verum anticancer activity against MDA-MB-231 cell line. Pak J Med Sci. 2024;40(1):---------. doi: https://doi.org/10.12669/pjms.40.1.7860 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionsupporting

confidence: 92%

Illicium verum anticancer activity against MDA-MB-231 cell line

Pahore,

Khan,

Karim

2023

Pak J Med Sci

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“…21 Petasites hybridus, Artemisia nilagirica, and Zanthoxylum armatum extracts are reported to exert anticancer action via apoptosis in MCF-7 cells, studied by DAPI dye staining assay. [22][23][24] In the present study, I. verum extract also caused significant induction of apoptosis at the IC 50 dose of 5.5µg/ml in MCF-7 cells, confirming that the cytotoxic effect of I. verum extract is due to automatic cell death. These findings are consistent with the results obtained from both cell viability and cell proliferation assays.…”

Section: Discussionsupporting

confidence: 81%

Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line

Pahore¹,

Khan

Karim³

2022

Pak J Med Sci

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Objective: To investigate the anticancer effect of Illicium verum against human breast cancer MCF-7 cell line. Methods: An experimental study was conducted in Multidisciplinary and Tissue Culture Laboratory, Aga Khan University in collaboration with Pharmacology Department of Bahria University Medical and Dental College, Karachi, Pakistan from January 2021 to June 2021. MCF-7 cells of Luminal-A breast cancer were seeded in 96-well plate and treated with I.verum methanol extract. After incubation, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) dye was used for cell viability and cell proliferation assays to determine the number of dead and viable cells, and the absorbance was measured using an enzyme-linked immunosorbent assay (ELISA) plate reader. In cell viability assay, different doses of I. verum methanol extract were used to treat the MCF-7 (0.25, 0.5, 1, 3, 6, 12, 25, and 50µg/ml) cells. For apoptosis analysis, the cells were processed with 4´, 6-diamidino-2-phenylindole fluorescent nuclear dye (DAPI) and were examined for fluorescence intensity and apoptotic cells. For cell proliferation assay and apoptosis the IC50 dose of 5.5µg/ml I. verum methanol extract was used. Results: The MCF-7 cells showed a significant reduction (p-value <0.01) in cell viability in the presence of all tested doses of I. verum methanol extract, except for the dose of 0.25µg/ml. The IC50 dose 5.5µg/ml of same extract also showed a significant reduction (p-value <0.01) in cell proliferation and apoptosis induction in MCF-7 cells. Conclusions: Illicium verum methanol extract possesses very potent anticancer action against MCF-7 cells through cytotoxicity, reduction, and inhibition of cancer cells and by inducing apoptosis. doi: https://doi.org/10.12669/pjms.39.1.6580 How to cite this: Pahore AK, Khan S, Karim N. Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line. Pak J Med Sci. 2023;39(1):---------. doi: https://doi.org/10.12669/pjms.39.1.6580 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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“…Halk hekimliğinde solunum yolu rahatsızlıkları, sindirim sistemi hastalıkları ve baş ağrısının tedavisinde kullanıldığı beliritilmektedir (26). Bitkiden elde edilen ekstraktların ve bileşiklerin migren, alerjik rinnit, meme kanseri ve SARS-CoV-2 üzerinde etkili olduğu bilinmektedir (27)(28)(29)(30). Ayıca bitki antimikrobiyal ve antioksidan etki göstermektedir (31).…”

Section: Kabalak Otu (Petasites Hybridus)unclassified

Karadeniz Bölgesinde yetişen bazı yenilebilir yabani otlar ve biyoaktif özellikleri

Gündüz,

Karabıyıklı Çiçek

2024

Toros University JFNG

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Karadeniz Bölgesi’nde dünya geneline yayılmış olan birçok bitki bir arada bulunmaktadır ve bölgede yabani otlara karşı büyük ilgi gösterilmektedir. Bölgenin yöresel yemeklerinde birçok farklı yabani ota yer verilmektedir. Yenilebilir yabani otlar sahip oldukları fenolik bileşikler, uçucu yağlar, amino asitler ve peptidler, organik asitler, vitaminler ve mineraller sebebiyle insan beslenmesinde önemli bir role sahip olmaktadır. Gıda endüstrisinde yenilebilir yabani otlar, matrislerinde bulunan biyoaktif bileşikler sayesinde temiz etiketli gıda üretimi için alternatif bir hammadde olmaktadır. Karadeniz Bölgesi’nin birçok farklı lokasyonunda sakarca (Ornithogalum umbellatum L.), kaldirik otu (Trachystemon orientalis (L.) G. Don), kabalak otu (Petasites hybridus), diken ucu (Smilax excelsa L.), gut otu (Aegopodium podagraria L.), hılak otu (Chaerophyllum byzantinum Boiss.), civcilik otu (Stellaria media L.), benli pancar (Polygonum lapathifolium L.), kazayağı otu (Falcaria vulgaris Bernh.), gıvışgan otu (Silene vulgaris) ve kedidili otu (Coronopus squamatus) yabani ot olarak yetişmektedir. Bu bitkilerden elde edilebilecek doğal gıda katkı maddelerinin gıdalardaki patojen mikroorganizmaların inhibisyonunda görev alması ve gıdaların organoleptik özelliklerinin muhafaza edilmesi amacıyla kullanımının mümkün olduğu öngörülmektedir. Bölge halkının beslenme alışkanlığında önemli bir yere sahip olmasına rağmen ulusal platformlarda ve gıda endüstrisinde ticari değere sahip olmayan bu otlar hakkındaki çalışmalara devam edilmekte ve böylelikle milli gelire katkı sağlanması amaçlanmaktadır. Bölgede yetişmekte olan yenilebilir yabani otlar hakkındaki farmakolojik çalışmalar, irdelenen bitkilerin, ilaç endüstrisi için de önemli kaynaklar olabileceğini ve modern tıpta birçok tedavi yöntemine entegre edilmesinin mümkün olabileceğini göstermektedir.

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Anti-cancer effect of Petasites hybridus L. (Butterbur) root extract on breast cancer cell lines

Cited by 7 publications

References 23 publications

Illicium verum anticancer activity against MDA-MB-231 cell line

Illicium verum anticancer activity against MDA-MB-231 cell line

Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line

Karadeniz Bölgesinde yetişen bazı yenilebilir yabani otlar ve biyoaktif özellikleri

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