Anti‐cancer effect ofCassia auriculataleaf extractin vitrothrough cell cycle arrest and induction of apoptosis in human breast and larynx cancer cell lines

Abstract

The in vitro anti-cancer effect of Cassia auriculata leaf extract (CALE) was evaluated in human breast adenocarcinoma MCF-7 and human larynx carcinoma Hep-2 cell lines. CALE preferentially inhibited the growth of both the cell lines in a dose-dependent manner with IC(50) values of 400 and 500 microg for MCF-7 and Hep-2 cells, respectively. The results showed the anti-cancer action is due to nuclear fragmentation and condensation, associated with the appearance of A(0) peak in cell cycle analysis that is indicative of apoptosis. In addition, CALE treated MCF-7 and Hep-2 cells had decreased expression of anti-apoptotic Bcl-2 protein and increased expression of pro-apoptotic Bax protein, eventually leading a decrease in the Bcl-2/Bax ratio. These results demonstrated that CALE inhibits the proliferation of MCF-7 and Hep-2 cells through induction of apoptosis, making CALE a candidate as new anti-cancer drug.

D-Pinitol Promotes Apoptosis in MCF-7 Cells via Induction of p53 and Bax and Inhibition of Bcl-2 and NF-κB

“…Metabolically active cells are able to convert the dye to water-insoluble dark blue formazan by reductive cleavage of the tetrazolium ring. The reduction of MTT is proportional to the number of active mitochondria in the live cells (Prasanna et al, 2009). From the present study the % of inhibition was reduced in D-pinitol treated cells.…”

Apoptosis induction by an analog of curcumin (BDMC-A) in human laryngeal carcinoma cells through intrinsic and extrinsic pathways

“…We observed upregulation of FasL, caspase-8 and caspase-3 expression upon curcumin and BDMC-A treatment, and found that this upregulation was more pronounced in BDMC-A treated cells, indicating its more potent ability to activate the extrinsic apoptotic pathway. We also found that BDMC-A treated Hep-2 cells showed nuclear fragmentation, which is another prominent feature of apoptosis [43], and that this nuclear fragmentation was more pronounced in BDMC-A treated cells compared to curcumin treated cells.…”

Antimalarial activities of medicinal plants traditionally used in the villages of Dharmapuri regions of South India

“…Previous reports demonstrate the antiplasmodial (Kamaraj et al, 2011) anti-diabetic (Gupta et al, 2010), larvicidal ) and anti-cancer (Prasanna et al, 2009) effects of leaf extracts of Cassia auriculata. Juan-Badaturuge et al (2011) isolated the antioxidant kaempferol-3-O-rutinoside, kaempferol, quercetin and luteolin from Cassia auriculata.…”