D-Pinitol Promotes Apoptosis in MCF-7 Cells via Induction of p53 and Bax and Inhibition of Bcl-2 and NF-κB

Rengarajan, Thamaraiselvan; Nandakumar, Natarajan; Rajendran, Peramaiyan; Haribabu, Lingaiah; Nishigaki, Ikuo; Balasubramanian, M.

doi:10.7314/apjcp.2014.15.4.1757

Cited by 53 publications

(35 citation statements)

References 36 publications

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“…The results of the present study demonstrated that curcumin was able to inhibit MCF-7 cell proliferation and promote apoptosis, possibly by regulating the NF-κB signaling pathway. It is well known that breast cancer is one of the most common malignancies in females and the main cause of cancer-associated mortality of females worldwide (14,15). In breast cancer the complicated process is able to cause the loss of normal regulatory pathways between cell proliferation, differe ntiation and apoptosis (3).…”

Section: Discussionmentioning

confidence: 99%

Curcumin inhibits MCF‑7 cells by modulating the NF‑κB signaling pathway

et al. 2017

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Abstract. The present study investigated the inhibitory effect of curcumin on human breast cancer MCF-7 cells and investigated the potential underlying molecular mechanisms. MCF-7 cells were cultured with curcumin at different concentrations and time points. The effects of curcumin treatment on breast cancer cell proliferation were studied using a MTT assay. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to assess the mRNA and protein expression levels of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (Bax), nuclear factor-κ-light-chain-enhancer of activated B cells (NF-κB) and inhibitor of NF-κB-α (IκBα). The proliferation of MCF-7 cells in the group treated with curcumin was markedly decreased compared with the control, with the greatest inhibitory effect at a concentration of 20 µM. The expression of Bax mRNA was increased and Bcl-2 mRNA expression was decreased compared with the control. Additionally, protein expression of NF-κB and IκB was increased. The data indicate that curcumin is able to inhibit breast cancer cell proliferation, possibly by regulating the NF-κB signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Curcumin inhibits MCF‑7 cells by modulating the NF‑κB signaling pathway

et al. 2017

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“…The earlier findings from our laboratory revealed that D-pinitol has excellent antioxidant activity which is involved in the prevention of elevation of lipid peroxidation which ultimately results in the protection of the cell membrane during DMBAinduced mammary carcinogenesis [47]. Several findings reported that D-pinitol could downregulate the NF-κB expression in several cancer cell lines [33,46]. Inflammation is a part of the defense response; chronic inflammation can lead to cancer.…”

Section: Introductionmentioning

confidence: 93%

d-pinitol mitigates tumor growth by modulating interleukins and hormones and induces apoptosis in rat breast carcinogenesis through inhibition of NF-κB

Rengarajan

Nandakumar

Rajendran³

et al. 2015

J Physiol Biochem

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Breast cancer is the most prevalent malignant neoplasm in the world, and chemoprevention through dietary intervention strategy is an emerging option to reduce the incidence. D-pinitol (DP), a major component of soya bean, possesses attractive biological actions. We have investigated whether D-pinitol have an effect on tumor growth in vivo against 7,12-dimethylbenz(a)anthracene (DMBA)-initiated rat mammary carcinogenesis and investigated its mechanism of action. Tumors were induced in Sprague-Dawley (SD) rats by a gastric dose of 20 mg/kg DMBA, and after 13 weeks of induction period, the rats were orally administered with D-pinitol for 45 days. At the end of the assay, animals in carcinogen control group prompted a tumor incidence of 100 % and developed a tumor volume of 8.35 ± 0.56, which was significantly reduced to 5.74 ± 0.32 for the animals treated with D-pinitol. The D-pinitol treatment not only decreased the tumor volume but also further examination revealed that tumors from animals that received D-pinitol reduced nuclear factor kappa B (NF-κB) activation which in turn results in modulation of its downstreaming p53 and proteins of caspase-3 family. Bcl-2 expression and caspase-3 activation were also decreased after D-pinitol supplementation leading to induction of apoptosis and finally cell death. Furthermore, the status of the inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin (IL)-2, IL-6, and tumor markers, lipid profile, and hormones was also significantly declined up on D-pinitol administration. Thus, it reveals the collective involvement of the above-mentioned parameters along with NF-κB signaling through which D-pinitol induces apoptosis and subsequently suppresses breast cancer during DMBA-induced rat breast carcinogenesis.

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“…P53 and Bax are apoptotic genes but Bcl-2 is an anti-apoptotic gene (29). The expression of P53 and Bax genes in the group treated with H 2 O 2 was highest compared to the other groups.…”

Section: Discussionmentioning

confidence: 99%

In vitro antiapoptotic effects of the calligonum extract on spermatogonial stem cells

Barati

Movahedin

Batooli

2018

IJRM

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Background:Spermatogonial stem cells are the foundation of spermatogenesis and male fertility. So, their maintenance and culture are very important.Objective:In this study, we assessed protective effects of the Calligonum on in vitro viability and apoptotic and antiapoptotic genes expression of spermatogonial stem cells.Materials and Methods:After 24 hr of culture, the spermatogonial stem cells were treated with 30 μM dose of H2O2 and then 10 μg/ml the Calligonum extract was added for 3 wks. Viability was assessed by Trypan blue, apoptosis using PI-Annexin and finally Bax, Bcl-2 and P53 genes expression by Real-Time Polymerase chain reaction.Results: After 3 wk of treatment, viability in the Calligonum extract+H2O2 group was significantly higher than H2O2 group alone (p=0.001). In the Calligonum extract+H2O2 group, apoptosis, as well as expression of apoptotic genes (Bax and P53), was significantly lower than the group treated with H2O2 alone.Conclusion:The results of this study showed that 30 μM H2O2 increased apoptosis but decreased viability in spermatogonial stem cells. Calligonum has antioxidant properties that can reduce apoptosis, Bax and P53 expression and increase the viability and Bcl-2 expression.

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D-Pinitol Promotes Apoptosis in MCF-7 Cells via Induction of p53 and Bax and Inhibition of Bcl-2 and NF-κB

Cited by 53 publications

References 36 publications

Curcumin inhibits MCF‑7 cells by modulating the NF‑κB signaling pathway

Curcumin inhibits MCF‑7 cells by modulating the NF‑κB signaling pathway

d-pinitol mitigates tumor growth by modulating interleukins and hormones and induces apoptosis in rat breast carcinogenesis through inhibition of NF-κB

In vitro antiapoptotic effects of the calligonum extract on spermatogonial stem cells

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