Anti-Cancer Drug Assay for Micro-Ellipse Filters after Sensitively Capturing Circulating Tumor Cells

Chen, Hongmei; Cao, Bangwei; Chen, Hongda; Yang, Hui; Liu, Peihua; Wang, Tengfei

doi:10.4172/2167-1044.s11-004

Cited by 1 publication

(1 citation statement)

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“…A syringe pump was used to load cell suspension into Spiral chip. The syringe attached to the pump was connected to the inlet of the triplet-microchannel spiral microfluidic chip through plastic tubing 30 . An inverted phase contrast fluorescence microscope (Nikon, Japan) equipped with a high-speed CCD camera (Nikon, Japan) was used to observe.…”

Section: Methodsmentioning

confidence: 99%

A Triplet Parallelizing Spiral Microfluidic Chip for Continuous Separation of Tumor Cells

Chen

2018

Sci Rep

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Inertial and deformability- based particles separations gradually attract more significant attentions. In this work, we present a hybrid chip by combining the advantages of inertial and deformability –based principle. The chip is a triplet parallelizing spiral inertial microfluidic chip interconnected with numerable tilted slits (Spiral-Slits Chip) for continuous separation of circulating tumor cells. Utilizing the inertial lift and viscous drag forces, different sized particles achieve different equilibrium at distinct streamlines of the spiral microchannel. Numerable tilted slits are organized along the flow direction. They frequently transport segregated streamline particles into a paralleled smaller microchannel. These frequent dragging results in the amount of certain sized particles in the original microchannel gradually and dramatically reduced. Inertial separation of distinct sized particles could be achievable. Two arrays of numerable tilted slits function as bridges. This Spiral-Slits Chip could substitute for Red Blood Cells Lysis (RBCL) and is most effective for ultra-high throughput. The overall arrangement of this triplet parallelizing spiral inertial microfluidic reflects stable streamlines distribution in the first main microchannel. Combining with Ellipse filters, robust and reproducible capture of CTCs could be achieved at high flow rates. Optical absorption detection has been tentatively tested, and this could simplify the process.

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Section: Methodsmentioning

confidence: 99%