A Triplet Parallelizing Spiral Microfluidic Chip for Continuous Separation of Tumor Cells

Chen, Hongmei

doi:10.1038/s41598-018-22348-z

Cited by 33 publications

(16 citation statements)

References 30 publications

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“…A novel inertial separation technique using spiral microchannel having a stair-like cross-section was introduced for size-based particle separation [98]. A spiral microfluidic chip was also employed for continuous separation of CTCs [99] and sperm-like-particles (SLPs) [100] from blood.…”

Section: Inertia and Dean Flowmentioning

confidence: 99%

Public-Health-Driven Microfluidic Technologies: From Separation to Detection

Zhang

Wang

et al. 2021

Micromachines

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Separation and detection are ubiquitous in our daily life and they are two of the most important steps toward practical biomedical diagnostics and industrial applications. A deep understanding of working principles and examples of separation and detection enables a plethora of applications from blood test and air/water quality monitoring to food safety and biosecurity; none of which are irrelevant to public health. Microfluidics can separate and detect various particles/aerosols as well as cells/viruses in a cost-effective and easy-to-operate manner. There are a number of papers reviewing microfluidic separation and detection, but to the best of our knowledge, the two topics are normally reviewed separately. In fact, these two themes are closely related with each other from the perspectives of public health: understanding separation or sorting technique will lead to the development of new detection methods, thereby providing new paths to guide the separation routes. Therefore, the purpose of this review paper is two-fold: reporting the latest developments in the application of microfluidics for separation and outlining the emerging research in microfluidic detection. The dominating microfluidics-based passive separation methods and detection methods are discussed, along with the future perspectives and challenges being discussed. Our work inspires novel development of separation and detection methods for the benefits of public health.

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Section: Inertia and Dean Flowmentioning

confidence: 99%

Public-Health-Driven Microfluidic Technologies: From Separation to Detection

Zhang

Wang

et al. 2021

Micromachines

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“…This method has been FDA-approved for advanced PCa [162], breast cancer [163] and CRC [164] metastasis studies. The other CTC isolation methods include chip-based isolation [165,166] and MagSweeper [167]. Alternative methods like micro-fluidic immunofluorescence [168] and microfluidic western blotting [169] detect a limited number of proteins in the CTC samples.…”

Section: Blood-based Proteomicsmentioning

confidence: 99%

Recent advances in mass spectrometry based clinical proteomics: applications to cancer research

2020

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Cancer biomarkers have transformed current practices in the oncology clinic. Continued discovery and validation are crucial for improving early diagnosis, risk stratification, and monitoring patient response to treatment. Profiling of the tumour genome and transcriptome are now established tools for the discovery of novel biomarkers, but alterations in proteome expression are more likely to reflect changes in tumour pathophysiology. In the past, clinical diagnostics have strongly relied on antibody-based detection strategies, but these methods carry certain limitations. Mass spectrometry (MS) is a powerful method that enables increasingly comprehensive insights into changes of the proteome to advance personalized medicine. In this review, recent improvements in MS-based clinical proteomics are highlighted with a focus on oncology. We will provide a detailed overview of clinically relevant samples types, as well as, consideration for sample preparation methods, protein quantitation strategies, MS configurations, and data analysis pipelines currently available to researchers. Critical consideration of each step is necessary to address the pressing clinical questions that advance cancer patient diagnosis and prognosis. While the majority of studies focus on the discovery of clinically-relevant biomarkers, there is a growing demand for rigorous biomarker validation. These studies focus on high-throughput targeted MS assays and multi-centre studies with standardized protocols. Additionally, improvements in MS sensitivity are opening the door to new classes of tumour-specific proteoforms including posttranslational modifications and variants originating from genomic aberrations. Overlaying proteomic data to complement genomic and transcriptomic datasets forges the growing field of proteogenomics, which shows great potential to improve our understanding of cancer biology. Overall, these advancements not only solidify MS-based clinical proteomics' integral position in cancer research, but also accelerate the shift towards becoming a regular component of routine analysis and clinical practice.

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“…Wang and coworkers recently combined deformability-based separation with magnetic-based techniques to separate CTCs, RBCs, and WBCs from "liquid biopsy" samples [114]. In 2018, Hongmei Chen reported the separation of CTCs, RBCs and WBCs from spiked peripheral blood samples using a combination of deformability-based and inertial separation [115]. Zhou et al predicted that it is possible to combine deformability-based and electrokinetic separation, which relies on different shear moduli instead of ratchets [116].…”

Section: Deformability-basedmentioning

confidence: 99%

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Hochstetter

2020

Micromachines

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In the last three decades, microfluidics and its applications have been on an exponential rise, including approaches to isolate rare cells and diagnose diseases on the single-cell level. The techniques mentioned herein have already had significant impacts in our lives, from in-the-field diagnosis of disease and parasitic infections, through home fertility tests, to uncovering the interactions between SARS-CoV-2 and their host cells. This review gives an overview of the field in general and the most notable developments of the last five years, in three parts: 1. What can we detect? 2. Which detection technologies are used in which setting? 3. How do these techniques work? Finally, this review discusses potentials, shortfalls, and an outlook on future developments, especially in respect to the funding landscape and the field-application of these chips.

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A Triplet Parallelizing Spiral Microfluidic Chip for Continuous Separation of Tumor Cells

Cited by 33 publications

References 30 publications

Public-Health-Driven Microfluidic Technologies: From Separation to Detection

Public-Health-Driven Microfluidic Technologies: From Separation to Detection

Recent advances in mass spectrometry based clinical proteomics: applications to cancer research

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

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