Anti-cancer, Anti-invasiveness &amp;amp; Anti-metastasis of Green Engineered Bioorganic-Capped Silver Nanoparticles Fabricated from Juniperus chinensis Extract and Comparison to Cisplatin in Lung Cancer Cells (A549): In Vitro Assessment of Cellular and Molecular Pathways

Noorbazargan, Hassan; Mashayekhi, Ainaz; Khayam, Nazanin; Naghizadeh, Mohammad Mehdi; Mirzaie, Amir; Dolatabadi, Aghigh; Moulavi, Pooria; amintehrani, Sobhan; Kavousi, Mahsa

doi:10.21203/rs.3.rs-88253/v1

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…The cells were stained with DAPI for 10 min after being washed in PBS. Ultimately, the cell morphologies were studied using an Olympus IX81 inverted fluorescence microscope with a DP72 digital camera (Olympus, Hamburg, Germany) [ 58 ].…”

Section: Methodsmentioning

confidence: 99%

Folate-Targeted Curcumin-Loaded Niosomes for Site-Specific Delivery in Breast Cancer Treatment: In Silico and In Vitro Study

et al. 2022

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As the most common cancer in women, efforts have been made to develop novel nanomedicine-based therapeutics for breast cancer. In the present study, the in silico curcumin (Cur) properties were investigated, and we found some important drawbacks of Cur. To enhance cancer therapeutics of Cur, three different nonionic surfactants (span 20, 60, and 80) were used to prepare various Cur-loaded niosomes (Nio-Cur). Then, fabricated Nio-Cur were decorated with folic acid (FA) and polyethylene glycol (PEG) for breast cancer suppression. For PEG-FA@Nio-Cur, the gene expression levels of Bax and p53 were higher compared to free drug and Nio-Cur. With PEG-FA-decorated Nio-Cur, levels of Bcl2 were lower than the free drug and Nio-Cur. When MCF7 and 4T1 cell uptake tests of PEG-FA@Nio-Cur and Nio-Cur were investigated, the results showed that the PEG-FA-modified niosomes exhibited the most preponderant endocytosis. In vitro experiments demonstrate that PEG-FA@Nio-Cur is a promising strategy for the delivery of Cur in breast cancer therapy. Breast cancer cells absorbed the prepared nanoformulations and exhibited sustained drug release characteristics.

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Section: Methodsmentioning

confidence: 99%

Folate-Targeted Curcumin-Loaded Niosomes for Site-Specific Delivery in Breast Cancer Treatment: In Silico and In Vitro Study

et al. 2022

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“…Ultimately, the cell morphologies were studied using an Olympus IX81 inverted fluorescence microscope with a DP72 digital camera (Olympus, Hamburg, Germany). 41 …”

Section: Methodsmentioning

confidence: 99%

Polyvinyl Alcohol (PVA)-Based Nanoniosome for Enhanced in vitro Delivery and Anticancer Activity of Thymol

Abdihaji,

Mirzaei Chegeni,

Hadizadeh

et al. 2023

IJN

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There is an unmet need to develop potent therapeutics against cancer with minimal side effects and systemic toxicity. Thymol (TH) is an herbal medicine with anti-cancer properties that has been investigated scientifically. This study shows that TH induces apoptosis in cancerous cell lines such as MCF-7, AGS, and HepG2. Furthermore, this study reveals that TH can be encapsulated in a Polyvinyl alcohol (PVA)-coated niosome (Nio-TH/PVA) to enhance its stability and enable its controlled release as a model drug in the cancerous region. Materials and Methods: TH-loaded niosome (Nio-TH) was fabricated and optimized using Box-Behnken method and the size, polydispersity index (PDI) and entrapment efficiency (EE) were characterized by employing DLS, TEM and SEM, respectively. Additionally, in vitro drug release and kinetic studies were performed. Cytotoxicity, antiproliferative activity, and the mechanism were assessed by MTT assay, quantitative real-time PCR, flow cytometry, cell cycle, caspase activity evaluation, reactive oxygen species investigation, and cell migration assays. Results: This study demonstrated the exceptional stability of Nio-TH/PVA at 4 °C for two months and its pH-dependent release profile. It also showed its high toxicity on cancerous cell lines and high compatibility with HFF cells. It revealed the modulation of Caspase-3/Caspase-9, MMP-2/MMP-9 and Cyclin D/ Cyclin E genes by Nio-TH/PVA on the studied cell lines. It confirmed the induction of apoptosis by Nio-TH/PVA in flow cytometry, caspase activity, ROS level, and DAPI staining assays. It also verified the inhibition of metastasis by Nio-TH/PVA in migration assays. Conclusion: Overall, the results of this study revealed that Nio-TH/PVA may effectively transport hydrophobic drugs to cancer cells with a controlled-release profile to induce apoptosis while exhibiting no detectable side effects due to their biocompatibility with normal cells.

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Fabrication, optimization, and characterization of pH-responsive PEGylated nanoniosomes containing gingerol for enhanced treatment of breast cancer

Lalami

Tafvizi

Naseh

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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Anti-cancer, Anti-invasiveness & Anti-metastasis of Green Engineered Bioorganic-Capped Silver Nanoparticles Fabricated from Juniperus chinensis Extract and Comparison to Cisplatin in Lung Cancer Cells (A549): In Vitro Assessment of Cellular and Molecular Pathways

Cited by 4 publications

References 31 publications

Folate-Targeted Curcumin-Loaded Niosomes for Site-Specific Delivery in Breast Cancer Treatment: In Silico and In Vitro Study

Folate-Targeted Curcumin-Loaded Niosomes for Site-Specific Delivery in Breast Cancer Treatment: In Silico and In Vitro Study

Polyvinyl Alcohol (PVA)-Based Nanoniosome for Enhanced in vitro Delivery and Anticancer Activity of Thymol

Fabrication, optimization, and characterization of pH-responsive PEGylated nanoniosomes containing gingerol for enhanced treatment of breast cancer

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