Anti-biofilm efficacy of silver nanoparticles against MRSA and MRSE isolated from wounds in a tertiary care hospital

Abstract

Our findings suggested that AgNPs can be exploited towards the development of potential anti-bacterial coatings for various biomedical and environmental applications. In the near future, the AgNPs may play major role in the coating of medical devices and treatment of infections caused due to highly antibiotic resistant biofilm.

Investigation of Biofilm Formation of ESBL Positive Uropathogenic Escherichia coli Isolates on (Microplate) Surfaces Modified with Plasma Polymerization Technique and Nanomaterials: An Experimental Model

“…Bunun nedeni monomerin kimyasal yapısının gram negatif bakterilerle oluşturduğu elektrostatik etkileşim olabilir. EGDM'nin gram pozitif bakteriler üzerinde inhibitör etkinliği olduğunu gösteren çalışmalar mevcuttur (35,36) .…”

Nanomedicine opened new horizons for metal nanoparticles to treat multi-drug resistant organisms

“…The anti-biofilm activity of metal nanoparticles was also demonstrated in other studies mainly focused on bacteria showing resistance to conventional antibiotics (88,89). The biofilm formation by methicillin resistant S. aureus (MRSA) and methicillin resistant S. epidermidis (MRSE) isolated from infected wounds was also analyzed by confocal laser scanning microscopy (CLSM) techniques which provided concrete evidence of the ability of NPs to block bacterial growth and to prevent the glycocalyx formation.…”

“…Along this line, magnetic and antibacterial properties have been exploited by creating super paramagnetic iron oxide nanoparticles (SPION)conjugated with metal to demonstrate that MRSA biofilms can be eradicated without the need of antibiotics (88).MRSA biofilms treated with 1 mg/mL of metal-conjugated SPION resulted in a consistent mass decrease (11,53,17) as shown in figure 3.Moreover, SPION anti-biofilm efficacy is further improved in the presence of an external magnetic field. Metalis nowadays used on medical devices to support anti-biofilm activity.…”

“…Metalis nowadays used on medical devices to support anti-biofilm activity. Biofilms from clinical isolates of P. aeruginosa were treated with gum arabic capped-metal nanoparticles (GA-NPs) showing a concentration dependent inhibition of bacterial growth and treatment of catheters with GA-NPs at50 μg/mL resulted in 95% inhibition of bacterial colonization of the plastic catheter surface (88).Other authors have also shown the applications of nanometal as antibiofilms for coating catheters with positive results against both Gram-positive and Gramnegative bacteria (92,94). Furthermore, no significant accumulation of metal was detected in the main organs of the test animals in which engineered catheters had been implanted (92).…”

Antibiotic Effect on Planktonic and Biofilm-Producing Staphylococci

“…Indeed, biofilm formation is a hallmark characteristic of infections caused by S. aureus and S. epidermidis, which consist of multiple bacterial layers encased within an exo-polysaccharide glycocalyx [11]. Some substances, including silver nanoparticles, have been used to decrease biofilm formation [12].…”