Anti-Biofilm and Anti-Hemolysis Activities of 10-Hydroxy-2-decenoic Acid against Staphylococcus aureus

Abstract

Persistent infections caused by Staphylococcus aureus biofilms pose a major threat to global public health. 10-Hydroxy-2-decenoic acid (10-HDA), a main fatty acid in royal jelly, has been shown to possess various biological activities. The purpose of this study was to explore the effects of 10-HDA on the biofilms and virulence of S. aureus and its potential molecular mechanism. Quantitative crystal violet staining indicated that 10-HDA significantly reduced the biofilm biomass at sub-minimum inhibitory concentration (MIC) levels (1/32MIC to 1/2MIC). Scanning electron microscope (SEM) observations demonstrated that 10-HDA inhibited the secretion of extracellular polymeric substances, decreased bacterial adhesion and aggregation, and disrupted biofilm architecture. Moreover, 10-HDA could significantly decrease the biofilm viability and effectively eradicated the mature biofilms. It was also found that the hemolytic activity of S. aureus was significantly inhibited by 10-HDA. qRT-PCR analyses revealed that the expressions of global regulators sarA, agrA, and α-hemolysin gene hla were downregulated by 10-HDA. These results indicate that 10-HDA could be used as a potential natural antimicrobial agent to control the biofilm formation and virulence of S. aureus.

Halloysite Nanotubes Decorated with Fe3O4 Nanoparticles and Tannic Acid for Effective Inhibition of E. coli Biofilm

“…According to research by Kang et al, 2018, the expression level of a gene involved in biofilm generation was not reduced . Also, in Gao et al, 2022, the expression of genes was not downregulated, and some were upregulated . This indicates that even though biofilm production is suppressed, cells express more genes to produce biofilm.…”

“…51 Also, in Gao et al, 2022, the expression of genes was not downregulated, and some were upregulated. 52 This indicates that even though biofilm production is suppressed, cells express more genes to produce biofilm. In the control group, the gene expression levels of f imA and csgA were low.…”

Discovery of Salifungin as a Repurposed Antibiotic against Methicillin-Resistant Staphylococcus aureus with Limited Resistance Development

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Royal Jelly as a Nutraceutical Natural Product with a Focus on Its Antibacterial Activity

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