Anti-biofilm activity of dalbavancin against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) isolated from human bone infection

Silva, Vanessa; Miranda, Carla; Bezerra, Mário; Antão, Helena Sofia; Guimarães, João Paulo; Prada, Justina; Pires, Isabel; Maltez, Luís; Pereira, José Eduardo López; Capelo, José Luís; Igrejas, Gilberto; Poeta, Patrícia

doi:10.1080/1120009x.2021.1911518

Cited by 11 publications

(6 citation statements)

References 27 publications

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“…All isolates were also resistant to ciprofloxacin, which reflects the high intake of ciprofloxacin by Portuguese patients since quinolones are one of the most prescribed antibiotics in hospitals to treat soft tissue infections [18]. Furthermore, these results are in accordance with other studies conducted in patients with MRSA infections in the same hospitals [18,19,38]. Resistance to macrolides and lincosamides was detected in 22 (78.5%) out of 28 isolates and was possibly conferred by the ermA (n = 2), ermB (n = 4) or ermC (n = 20) or a combination of those genes.…”

Section: Antimicrobial Resistance and Virulencesupporting

confidence: 87%

High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater

et al. 2022

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Hospital wastewaters often carry multidrug-resistant bacteria and priority pathogens, such as methicillin-resistant Staphylococcus aureus (MRSA). Pathogens and antibiotic resistance genes present in wastewaters may reach the natural environment facilitating their spread. Thus, we aimed to isolate MRSA from wastewater of 3 hospitals located in the north of Portugal and to characterize the isolates regarding the antimicrobial resistance and genetic lineages. A total of 96 wastewater samples were collected over six months. The water was filtered, and the filtration membrane was immersed in BHI broth supplemented with 6.5% of NaCl and incubated. The inoculum was streaked in ORSAB agar plates for MRSA isolation. The isolates susceptibility testing was performed against 14 antimicrobial agents. The presence of resistance and virulence genes was accessed by PCR. Molecular typing was performed in all isolates. From the 96 samples, 28 (29.2%) were MRSA-positive. Most isolates had a multidrug-resistant profile and carried the mecA, blaZ, aac(6′)-Ie-aph(2″)-Ia, aph(3′)-IIIa, ermA, ermB, ermC, tetL, tetM, dfrA dfrG and catpC221 genes. Most of the isolates were ascribed to the immune evasion cluster (IEC) type B. The isolates belonged to ST22-IV, ST8-IV and ST105-II and spa-types t747, t1302, t19963, t6966, t020, t008 and tOur study shows that MRSA can be found over time in hospital wastewater. The wastewater treatment processes can reduce the MRSA load. The great majority of the isolates belonged to ST22 and spa-type t747 which suggests the fitness of these genetic lineages in hospital effluents.

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Section: Antimicrobial Resistance and Virulencesupporting

confidence: 87%

High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater

et al. 2022

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“…Of note, the inhibition of biofilm formation tends to increase upon longer exposure over 48 h of PMZ treatment. A similar biofilm detachment effect was also observed in MRSA cells treated with dalbavancin (0.4 mg/mL) 46 . In order to evaluate the cytotoxic effect of PMZ on human tissue, a hemolysis assay was performed using both human and chicken blood tissues 32 ; the results showed no hemolytic effect on human and chicken blood tissues, and the effect was found to be similar to the negative control.…”

Section: Discussionsupporting

confidence: 57%

“…A similar biofilm detachment effect was also observed in MRSA cells treated with dalbavancin (0.4 mg/mL). 46 In order to evaluate the cytotoxic effect of PMZ on human tissue, a hemolysis assay was performed using both human and chicken blood tissues 32 ; the results showed no hemolytic effect on human and chicken blood tissues, and the effect was found to be similar to the negative control. C. elegans is a promising model system for evaluating microbial pathogen infections and determination of antibacterial and anti-infective prosperity of tested compounds.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial effect of pimozide by targeting ROS‐mediated killing in Staphylococcus aureus

Kumar

Kharb

Kumar

et al. 2023

Biotech and App Biochem

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In spite of the higher nosocomial and community‐acquired infections caused by Staphylococcus aureus, emerging drug resistance is a leading cause of increased mortality and morbidity associated with the overuse of antimicrobials. It is an emergent need to find out new molecules to combat such infections. In the present study, we analyzed the antibacterial effect of pimozide (PMZ) against gram‐positive and gram‐negative bacterial strains, including methicillin‐sensitive (MSSA) and methicillin‐resistant (MRSA) S. aureus. The growth of MSSA and MRSA was completely inhibited at concentrations of 12.5 and 100 μg/mL, respectively, which is referred to as 1× minimum inhibitory concentration (MIC). The cell viability was completely eliminated within 90 min of PMZ treatment (2× MIC) through reactive oxygen species (ROS)‐mediated killing without affecting cell membrane permeability. It suppressed α‐hemolysin production and biofilm formation of different S. aureus strains by almost 50% at 1× MIC concentration, and was found to detach matured biofilm. PMZ treatment effectively eliminates S. aureus infection in Caenorhabditis elegans and improves its survival by 90% and is found safe to use with no hemolytic effect on human and chicken blood tissues. Taken together, it is concluded that PMZ may turn out to be an effective antibacterial for treating bacterial infections including MSSA and MRSA.

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“…Lipoglycopeptides are another class of antibiotics that arrest peptidoglycan synthesis. In particular, the lipoglycopeptide dalbavancin eradicates biofilms formed by S. aureus (MBEC 90 = 2 µg ml −1 ), S. epidermidis (MBEC 90 = 4 µg ml −1 ), and enterococci (MBECs for vancomycin-susceptible and -resistant isolates were ⩽4 and >16 µg ml −1 ) but is inefficient against vancomycin-resistant strains biofilms (Fernández et al 2016, Neudorfer et al 2018, Silva et al 2021. As catheters offer optimal conditions for bacterial adhesion, they are often at the origin of biofilm formation (Costerton et al 1999).…”

Section: Cell Wall Targetsmentioning

confidence: 99%

Antibiofilm approaches as a new paradigm for treating infections

Reffuveille,

Dghoughi,

Colin

et al. 2024

Prog. Biomed. Eng.

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The lack of effective antibiotics for drug-resistant infections has led the World Health Organization (WHO) to declare antibiotic resistance a global priority. Most bacterial infections are caused by microbes growing in structured communities called biofilms. Bacteria growing in biofilms are less susceptible to antibiotics than their planktonic counterparts. Despite their significant clinical implications, bacterial biofilms have not received the attention they warrant, with no approved antibiotics specifically designed for their eradication. In this paper, we aim to shed light on recent advancements in antibiofilm strategies that offer compelling alternatives to traditional antibiotics. Additionally, we will briefly explore the potential synergy between computational approaches, including the emerging field of artificial intelligence, and the accelerated design and discovery of novel antibiofilm molecules in the years ahead.

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Anti-biofilm activity of dalbavancin against methicillin-resistant Staphylococcus aureus (MRSA) isolated from human bone infection

Cited by 11 publications

References 27 publications

High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater

High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater

Antimicrobial effect of pimozide by targeting ROS‐mediated killing in Staphylococcus aureus

Antibiofilm approaches as a new paradigm for treating infections

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