Anti-biofilm Activities from Resveratrol against Fusobacterium nucleatum

He, Zhaojian; Huang, Zhengwei; Wei, Zhou; Tang, Zisheng; Ma, Rui; Ji, Liang

doi:10.3389/fmicb.2016.01065

Cited by 34 publications

(32 citation statements)

References 51 publications

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“…We showed that resveratrol exhibits antibacterial activity against P. gingivalis with a MIC of 250 µg/ml. This finding was consistent with previous studies, which reported that resveratrol inhibits the growth of other periodontopathogens including Aggregatibacter actinomycetemcomitans and F. nucleatum (He et al, ; O'Connor, Wong, & Rabie, ; Phoolcharoena et al, ). Since the over‐growth of P. gingivalis promotes a dysbiotic microbiota and an inflammatory microenvironment in periodontal pockets, the anti‐ P. gingivalis activity of resveratrol may contribute to attenuate the pathogenic process of periodontitis.…”

Section: Discussionsupporting

confidence: 92%

“…The mechanism involved in biofilm killing is likely related to the ability of resveratrol to alter the integrity of the bacterial cell membrane, as mentioned above. Interestingly, in a previous study, He et al () reported that resveratrol significantly decreases biofilm formation by F. nucleatum and suggested that the mechanism by which resveratrol exerts its effect on F. nucleatum biofilm formation at sub‐MICs is distinct from the mode of action by which resveratrol inhibits growth.…”

Section: Discussionmentioning

confidence: 97%

“…Moreover, recent studies have demonstrated that resveratrol exhibits antimicrobial activity. It can also reduce biofilm formation by human pathogens such as Vibrio cholera, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and the oral pathogen Fusobacterium nucleatum (Augustine, Goel, Sivakumar, Kumar, & Thomas, 2014;He et al, 2016;Lee, Kim, Ryu, Cho, & Lee, 2014;Qin et al, 2014). Based on our knowledge of the P. gingivalis-mediated periodontal tissue destruction, it can be proposed that resveratrol by acting directly on P. gingivalis and on host cells, including oral keratinocytes and monocytes, may be of interest for periodontal disease prevention and therapy.…”

Section: Discussionmentioning

confidence: 99%

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Resveratrol attenuates the pathogenic and inflammatory properties of Porphyromonas gingivalis

Lagha

Andrian

Grenier

2019

Molecular Oral Microbiology

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Porphyromonas gingivalis has been strongly associated with chronic periodontitis, which affects tooth‐supporting tissues. This Gram‐negative anaerobic bacterium produces a repertoire of virulence factors that modulate tissue destruction directly or indirectly by the induction of inflammatory processes. The aim of this study was to investigate the effects of resveratrol, a major polyphenol found in grapes and wine, on the growth and virulence properties of P. gingivalis as well as on gingival keratinocyte tight junction integrity and the host inflammatory response. Resveratrol exhibited antibacterial activity that may result from damage to the bacterial cell membrane. Resveratrol also killed a pre‐formed P. gingivalis biofilm and reduced bacterial adherence to matrix proteins. In addition, resveratrol had a protective effect on the integrity of the keratinocyte tight junctions by inhibiting its breakdown by P. gingivalis. This may be related to the ability of resveratrol to inhibit the protease activities of P. gingivalis. Lastly, resveratrol reduced P. gingivalis‐mediated activation of the NF‐κB signaling pathway and attenuated TREM‐1 gene expression as well as soluble TREM‐1 secretion in monocytes. The effect on NF‐κB activation likely results from the ability of resveratrol to act as a PPAR‐γ agonist. In summary, the antibacterial, anti‐adherence, and antiprotease properties of resveratrol, as well as its ability to protect the gingival keratinocyte barrier and attenuate the inflammatory response in monocytes suggest that it may be a promising novel therapeutic agent for treating periodontal disease.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Resveratrol attenuates the pathogenic and inflammatory properties of Porphyromonas gingivalis

Lagha

Andrian

Grenier

2019

Molecular Oral Microbiology

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“…F. nucleatum is regarded as a central organism for dental biofilm maturation due to its wide ability to aggregate with other microorganisms, such as Porphyromonas gingivalis [Tavares et al 2018]. It is considered as a bridge bacterium between early and late colonizers in dental plaque [He et al 2016]. The eventuality of H. pylori-and non H. pylory biofilm formation in the gastric environment has been raised [Rizzato et al 2019].…”

Section: Cohort Specific Speciesmentioning

confidence: 99%

The Presence of Periodontal Pathogens in Gastric Cancer

Leeuw¹,

Duval²

2020

Preprint

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BackgroundThe microbiome is thought to play a role in the development of gastric cancer (GC). Several studies have put forward putatively carcinogenic species in addition to Helicobacter pylori, but are not in perfect alignment, possibly due to variable parameters in the experiments, including downstream processing. Meta-analyses have not been published so far, so there is lack in clinical guidance beyond H. pylori eradication therapy.MethodsHere, we analysed gastric mucosa samples from nine public data sets, including GC samples. Using both unsupervised and supervised learning, we defined fine grain bacterial networks of gastric mucosa and identified species associated with tumor status of samples.ResultsWe found anatomic locations and cohort regions among the possible factors leading to the observation of study specific gastric microbiomes. Despite this variability, the periodontal species Fusobacterium nucleatum, Parvimonas micra and Peptostreptococcus stomatis were found in association with tumor status in several datasets. The three species were predicted to be in interaction by ecological network analysis and also formed the intersection of tumor associated species between four GC data sets and five colorectal cancer (CRC) data sets we reanalyzed. We formulated a probiotic composition putatively competing with the GC pathogen spectrum, from correlation analysis in a large superset of gut samples (n=17,800) from clinical- and crowd-sourced studies.ImplicationsThe overlapping bacterial pathogen spectrum between two gastrointestinal tumor types, GC and CRC, has implications for etiology, treatment and prevention. In vitro testing results reported in literature suggest H. pylori eradication treatment should be efficient against the GC pathogen spectrum, yet the existence of an upstream periodontal reservoir is of concern. To address this, we propose longer term use of the formulated probiotics composition.

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“…The growth inhibition assay was performed according to the previous method [19]. After amplifying in BHI media, the F. nucleatum was harvested by centrifugation and adjusted to a final concentration at an OD of 650nm of 0.5 (10^9 CFU/ml) in the AS.…”

Section: The Growth Inhibition Of F Nucleatummentioning

confidence: 99%

Effect of IgY on Periodontitis and Halitosis Induced by Fusobacterium nucleatum

Wang

Qiao

Bao

et al. 2019

Journal of Microbiology and Biotechnology

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Fusobacterium nucleatum is a morbific agent in periodontitis and halitosis. Egg yolk antibody (IgY) was obtained from egg yolks from chickens stimulated with F. nucleatum. This study was to assess the effectiveness of IgY on periodontitis and halitosis caused by F. nucleatum in vitro and in vivo. The growth of F. nucleatum was inhibited (p < 0.05) by different concentrations of IgY in vitro and the results of a Halimeter show volatile sulfur compounds (VSCs) were reduced to 904 ± 57 ppb at a concentration 40 mg/ml of IgY. The changes of fatty acids of F. nucleatum were determined using GC-MS. The scores for odor index of rat saliva were decreased. The major constituent of volatile organic compounds (VOCs) including short-chain acids decreased 46.2% in 10 mg/ml IgY, ammonia decreased 70% in 40 mg/ml IgY, while aldehydes and olefine ketones were almost unchanged. The ELISA assay revealed that IL-6 and TNF-α were decreased after 4 weeks' IgY treatment. Morphometric (X-ray) and histological analyses (HE) showed that IgY reduced alveolar bone loss and collagen fibers became orderly in rat models. As a result, IgY may have the potential to treat periodontitis and halitosis. 312 Wang et al. J. Microbiol. Biotechnol.

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Anti-biofilm Activities from Resveratrol against Fusobacterium nucleatum

Cited by 34 publications

References 51 publications

Resveratrol attenuates the pathogenic and inflammatory properties of Porphyromonas gingivalis

Resveratrol attenuates the pathogenic and inflammatory properties of Porphyromonas gingivalis

The Presence of Periodontal Pathogens in Gastric Cancer

Effect of IgY on Periodontitis and Halitosis Induced by Fusobacterium nucleatum

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