Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using Citrobacter spp. MS5 Culture Supernatant

Mondal, Aftab Hossain; Yadav, Dhananjay; Ali, Asghar; Khan, Noor Zaman; Haq, Qazi Mohd. Rizwanul

doi:10.3390/biom10060944

Cited by 21 publications

(13 citation statements)

References 48 publications

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“…The AgNPs were dispersed in deionized water and a drop of suspension was placed on carbon-coated copper grids as well as evaporated before being used to take TEM images. 25 The surface morphology of powdered AgNPs was confirmed by SEM analysis (ZEISS FE-SEM). Further, TEM and SEM micrographs were analyzed by Image J software (National Institutes of Health, Bethesda, MD, USA) to determine the size range and average size of nanoparticles.…”

Section: Characterization Of Agnpsmentioning

confidence: 99%

“…10,13,17,[21][22][23] The AgNPs have been also reported as potential antibacterial against multidrug-resistant bacteria like E. coli, S. aureus, Bacillus cereus, Enterobacter hormaechei, K. pneumoniae and P. aeruginosa. 1,19,24,25 The mode of antibacterial activity of AgNPs has been proposed by various authors as its ability to act on multiple target sites including outer LPS, cell wall, cell membrane, proteins and DNA, leading to cell death. 15,20,24,26,27 Due to the wide application of metal nanoparticles in various fields, the research on the synthesis of various metal nanoparticles has increased significantly during the last decade.…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of Silver Nanoparticles Using Culture Supernatant of Shewanella sp. ARY1 and Their Antibacterial Activity

Mondal

Yadav

Mitra

et al. 2020

IJN

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Purpose In this study, silver nanoparticles (AgNPs) were biosynthesized using culture supernatant of strain Shewanella sp. ARY1, characterized and their antibacterial activity was investigated against Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae. Methods The strain Shewanella sp. ARY1 was isolated from river Yamuna, Delhi and used for biosynthesis of AgNPs via extracellular approach. Biosynthesized AgNPs were characterized by UV-Visible (UV-Vis) spectrophotometer, fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray (EDX), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Antibacterial activity of AgNPs was determined by well diffusion, broth microdilution and streaking plate assay to determine the zone of inhibition (ZOI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), respectively. The effect of AgNPs on treated bacteria was investigated by electron microscopy analysis. Further, the biocompatibility of AgNPs was tested against mice erythrocytes (RBC) by hemolytic assay. Results The UV-Vis spectral analysis revealed absorption maxima at 450 nm which confirmed the formation of AgNPs. The FTIR analysis suggested the involvement of various supernatant biomolecules, as reducing and capping agents in the synthesis of AgNPs. The XRD and EDX analysis confirmed the crystalline and metallic nature of AgNPs, respectively. The TEM and SEM analysis showed nanoparticles were spherical with an average size of 38 nm. The biosynthesized AgNPs inhibited the growth and formed a clear zone of inhibition (ZOI) against tested Gram-negative strains. The MIC and MBC were determined as 8-16 µg/mL and 32 µg/mL, respectively. Further, electron microscopy analysis of treated cells showed that AgNPs can damage the outer membrane, release of cytoplasmic contents, and alter the normal morphology of Gram-negative bacteria, leading to cell death. The hemolytic assay indicated that the biosynthesized AgNPs were biocompatible at low dose concentrations. Conclusion This study demonstrates an eco-friendly process for extracellular synthesis of AgNPs using Shewanella sp. ARY1 and these AgNPs exhibited excellent antibacterial activity, which may be used to combat Gram-negative pathogens.

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Section: Characterization Of Agnpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biosynthesis of Silver Nanoparticles Using Culture Supernatant of Shewanella sp. ARY1 and Their Antibacterial Activity

Mondal

Yadav

Mitra

et al. 2020

IJN

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“…The AgNPs have been also reported as potential antibacterial agent against multidrug-resistant bacteria like Salmonella Typhimurium , Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus, Enterobacter hormaechei, Klebsiella pneumoniae etc. [ 1 , 2 , 6 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Mediated Rapid and Facile Synthesis of Silver Nanoparticles and Their Antimicrobial Efficacy against Pathogenic Microorganisms

Huq

Akter

2021

Materials

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In the present study, silver nanoparticles (AgNPs), biosynthesized using culture supernatant of bacterial strain Paenarthrobacter nicotinovorans MAHUQ-43, were characterized and their antimicrobial activity was investigated against both Gram-positive Bacillus cereus and Gram-negative bacteria Pseudomonas aeruginosa. Bacterial-mediated synthesized AgNPs were characterized by UV-Visible (UV-Vis) spectrophotometer, field emission-transmission electron microscopy (FE-TEM), energy dispersive X-ray (EDX), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) analysis. The UV-Vis spectral analysis showed the absorption maxima at 466 nm which assured the synthesis of AgNPs. The FE-TEM analysis revealed the spherical shape of nanoparticles with the size range from 13 to 27 nm. The EDX and XRD analysis ensured the crystalline nature of biosynthesized AgNPs. The FTIR analysis revealed the involvement of different biomolecules for the synthesis of AgNPs as reducing and capping agents. The bacterial-mediated synthesized AgNPs inhibited the growth of pathogenic strains B. cereus and P. aeruginosa and developed a clear zone of inhibition (ZOI). The MIC and MBC for both pathogens were 12.5 µg/mL and 25 µg/mL, respectively. Moreover, field emission scanning electron microscopy analysis revealed that the synthesized AgNPs can destroy the outer membrane and alter the cell morphology of treated pathogens, leading to the death of cells. This study concludes the eco-friendly, facile and rapid synthesis of AgNPs using P. nicotinovorans MAHUQ-43 and synthesized AgNPs showed excellent antimicrobial activity against both Gram-positive and Gram-negative pathogens.

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“…These NPs can be used in diverse fields, including the production of third generation biosensors [ 37 ], biofilm development [ 38 ], biolabeling and cell imaging [ 39 ], sensoristic devices [ 40 ] and diagnostics [ 41 ]. Moreover, biofabricated NPs showed promising applications as anticancer agents [ 31 , 42 , 43 , 44 , 45 , 46 , 47 , 48 ], antimicrobials [ 31 , 42 , 43 , 49 , 50 , 51 , 52 ], antioxidants [ 44 ], anticoagulants [ 46 ] and antimigration and antiproliferative agents [ 45 ].…”

Section: Strategies For Synthesis Of Nanoparticles Using Microbesmentioning

confidence: 99%

Microbe-Mediated Biosynthesis of Nanoparticles: Applications and Future Prospects

et al. 2021

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Nanotechnology is the science of nano-sized particles/structures (~100 nm) having a high surface-to-volume ratio that can modulate the physical, chemical and biological properties of the chemical compositions. In last few decades, nanoscience has attracted the attention of the scientific community worldwide due to its potential uses in the pharmacy, medical diagnostics and disease treatment, energy, electronics, agriculture, chemical and space industries. The properties of nanoparticles (NPs) are size and shape dependent. These characteristic features of nanoparticles can be explored for various other applications such as computer transistors, chemical sensors, electrometers, memory schemes, reusable catalysts, biosensing, antimicrobial activity, nanocomposites, medical imaging, tumor detection and drug delivery. Therefore, synthesizing nanoparticles of desired size, structure, monodispersity and morphology is crucial for the aforementioned applications. Recent advancements in nanotechnology aim at the synthesis of nanoparticles/materials using reliable, innoxious and novel ecofriendly techniques. In contrast to the traditional methods, the biosynthesis of nanoparticles of a desired nature and structure using the microbial machinery is not only quicker and safer but more environmentally friendly. Various microbes, including bacteria, actinobacteria, fungi, yeast, microalgae and viruses, have recently been explored for the synthesis of metal, metal oxide and other important NPs through intracellular and extracellular processes. Some bacteria and microalgae possess specific potential to fabricate distinctive nanomaterials such as exopolysaccharides, nanocellulose, nanoplates and nanowires. Moreover, their ability to synthesize nanoparticles can be enhanced using genetic engineering approaches. Thus, the use of microorganisms for synthesis of nanoparticles is unique and has a promising future. The present review provides explicit information on different strategies for the synthesis of nanoparticles using microbial cells; their applications in bioremediation, agriculture, medicine and diagnostics; and their future prospects.

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Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using Citrobacter spp. MS5 Culture Supernatant

Cited by 21 publications

References 48 publications

<p>Biosynthesis of Silver Nanoparticles Using Culture Supernatant of <em>Shewanella</em> sp. ARY1 and Their Antibacterial Activity</p>

<p>Biosynthesis of Silver Nanoparticles Using Culture Supernatant of <em>Shewanella</em> sp. ARY1 and Their Antibacterial Activity</p>

Bacterial Mediated Rapid and Facile Synthesis of Silver Nanoparticles and Their Antimicrobial Efficacy against Pathogenic Microorganisms

Microbe-Mediated Biosynthesis of Nanoparticles: Applications and Future Prospects

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