Anti-bacterial and anti-biofilm efficacies of bioinspired gold nanoparticles

Salam, Farook Dharul; Vinita, Manimaran Nadar; Patel, Parimal; Prakash, S.; Yuvakkumar, R.; Kumar, Ponnuchamy

doi:10.1016/j.matlet.2019.126998

Cited by 17 publications

(13 citation statements)

References 13 publications

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“…Moreover, the colonization of both the bacteria were also remarkably inhibited on the glass surface. A similar finding was reported earlier by green synthesized gold nanoparticles (Dharul Salam et al, 2020). A similar study has found that silver nanoparticles significantly decreased the viable number of cells within biofilms of S. aureus and P. aeruginosa in which even smaller concentrations (2.5 to 6.5 μg/ml) silver nanoparticles sufficiently decreased the bacterial viability up to 90% (Kostenko, Lyczak, Turner, & Martinuzzi, 2010).…”

Section: Confocal Microscopysupporting

confidence: 89%

Green synthesis of cerium oxide nanoparticles using Acorus calamus extract and their antibiofilm activity against bacterial pathogens

Altaf

Manoharadas

Zeyad

2021

Microscopy Res & Technique

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The emergence of multidrug resistance in bacterial pathogens has increased drastically and it has become prevalent in clinical infections. In last few decades, there is a large gap in the discovery of new antibiotics with novel mode of action. The situation of antimicrobial resistance has become so alarming that if not action is taken, infectious diseases will become major cause of global mortality and morbidity by 2050.The growing interest of researchers in nanotechnology and their possible application in healthcare is being seen as a new hope in discovery of novel antimicrobial agents. Among various approaches employed for the nanoparticle synthesis, biological methods are considered more advantageous and environment friendly. Biofilms are considered as novel target for the development of new antimicrobial entities. In this study, cerium oxide nanoparticles (CeO 2 -NPs) were synthesized using Acorus calamus aqueous extract and tested for the antibiofilm activity both against Gram +ve and Gram −ve bacteria. The average size of synthesized CeO 2 -NPs was found to be 22.03 nm. The biofilms of the test bacteria were inhibited by more than 75% by the treatment with CeO 2 -NPs. The quantitative biofilm data were further verified by light microscopy, electron microscopy, and confocal microscopy. The confocal and electron microscopic analysis confirmed that treatment with CeO2-NPs reduced the development and colonization of the bacteria on solid support. Moreover, it was found that the colonization and biofilm development by test bacteria were fairly reduced on the glass surface. Moreover, a dose-dependent inhibition of preformed biofilms was also found. The exopolysaccharides (EPS) production by the test bacteria were substantially reduced by the supplementation of CeO 2 -NPs in culture media.The findings of this study highlight the efficacy of cerium oxide nanoparticles against bacterial pathogens that may be exploited for the development of new alternative antimicrobial agent.

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Section: Confocal Microscopysupporting

confidence: 89%

Green synthesis of cerium oxide nanoparticles using Acorus calamus extract and their antibiofilm activity against bacterial pathogens

Altaf

Manoharadas

Zeyad

2021

Microscopy Res & Technique

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“…The color of the HAuCl 4 solution changed from transparent to light or ruby red, indicating the formation of gold nanoparticles (AuNPs-CA). 25 The visible red color is due to the reduction of Au +3 to Au 0 . 24 24 The UV–vis absorption spectrum of AuNPs-CA is shown in Figure 1 A.…”

Section: Resultsmentioning

confidence: 99%

“…This finding is in agreement with an earlier report where gold nanoparticles synthesized using the marine plant, Padina tetrastromatica , exhibited SPR at 526 nm. 25 The synthesis of gold nanoparticles using pulp extract of C. annuum has been documented in the literature. 26 The nanoparticles were triangular and hexagonal in shape, and some spherical particles were also observed when synthesized at different pH, temperature, and HAuCl 4 concentrations.…”

Section: Resultsmentioning

confidence: 99%

“…The results are in agreement with a previous finding where gold nanoparticles synthesized using a marine plant, P. tetrastromatica , inhibited the biofilm development of Staphylococcus aureus . 25 …”

Section: Resultsmentioning

confidence: 99%

“…A similar finding has been reported in which the inhibition of biofilms of P. aeruginosa by green synthesized gold nanoparticles was shown by fluorescence microscopy. 25 …”

Section: Resultsmentioning

confidence: 99%

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Biofabrication of Gold Nanoparticles Using Capsicum annuum Extract and Its Antiquorum Sensing and Antibiofilm Activity against Bacterial Pathogens

et al. 2021

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The intensive use of antimicrobial agents has led to the emergence of multidrug resistance (MDR) among microbial pathogens. Such microbial (MDR) infections become more problematic in chronic diseases in which the efficacy of chemotherapeutic agents is highly reduced. To combat the problem of drug resistance, inhibition of bacterial quorum sensing (QS) and biofilms are considered as promising strategies in the development of anti-infective agents. In this study, gold nanoparticles (AuNPs-CA) were biofabricated using Capsicum annuum aqueous extract and characterized. The AuNPs-CA were tested against the QS-controlled virulence factors and biofilms of Pseudomonas aeruginosa PAO1 and Serratia marcescens MTCC 97. AuNPs-CA were found to be crystalline in nature with average particle size 19.97 nm. QS-mediated virulent traits of P. aeruginosa PAO1 such as pyocyanin, pyoverdin, exoprotease activity, elastase activity, rhamnolipids production, and swimming motility were reduced by 91.94, 72.16, 81.82, 65.72, 46.66, and 46.09%, respectively. Similarly, dose-dependent inhibition of virulence factors of S. marcescens MTCC 97 was recorded by the treatment of AuNPs-CA. The biofilm development and exopolysaccharide (EPS) production also decreased significantly. Microscopic analysis revealed that the adherence and colonization of the bacteria on solid support were reduced to a remarkable extent. The findings indicate the possibility of application of green synthesized gold nanoparticles in the management of bacterial infection after careful in vivo investigation.

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Inorganic nanoparticles: An effective antibiofilm strategy

Tanwar,

Parauha,

There

et al. 2024

Luminescence

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Biofilm is a common problem associated with human health. Pathogenicity and increase in resistance of bacteria require urgent development of effective ways for the treatment of bacterial diseases. Different strategies have been developed for the treatment of bacterial infections among which nanoparticles have shown greater prospects in battling with infections. Biofilms are resistant microbial colonies that possess resistance and, hence, cannot be killed by conventional drugs. Nanoparticles offer new avenues for treating biofilm‐related infections involving multi‐drug resistant organisms. They possess great antibiofilm properties, disrupting cell architecture and preventing colony formation. Green‐synthesised nanoparticles are more effective and less toxic to human cells than commercially available or chemically synthesised antibiofilm nanoparticles. This review summarises the antibiofilm efficiency of plant‐mediated nanoparticles and knowledge about biofilm inhibition.

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Anti-bacterial and anti-biofilm efficacies of bioinspired gold nanoparticles

Cited by 17 publications

References 13 publications

Green synthesis of cerium oxide nanoparticles using Acorus calamus extract and their antibiofilm activity against bacterial pathogens

Green synthesis of cerium oxide nanoparticles using Acorus calamus extract and their antibiofilm activity against bacterial pathogens

Biofabrication of Gold Nanoparticles Using Capsicum annuum Extract and Its Antiquorum Sensing and Antibiofilm Activity against Bacterial Pathogens

Inorganic nanoparticles: An effective antibiofilm strategy

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