Anti-asthmatic effects of Angelica dahurica against ovalbumin-induced airway inflammation via upregulation of heme oxygenase-1

Lee, Mee-Young; Seo, Chang‐Seob; Lee, Jin-Ah; Lee, Nam-Hun; Kim, Jung Hoon; Ha, Hyekyung; Zheng, Ming; Son, Jong‐Keun; Shin, Hyeun‐Kyoo

doi:10.1016/j.fct.2010.12.004

Cited by 77 publications

(55 citation statements)

References 41 publications

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“…Furthermore, it inhibits the production of prostaglandin E 2 (17) and nitric oxide (NO) (18,19), and reduces the release of histamine (20). In addition, the ethanolic extract of A. dahurica displayed anti-inflammatory effects by the upregulation of heme oxygenase-1, particularly in mice with asthma (21,22). Phytochemical investigations have revealed that there were coumarins (23,24) in A. dahurica as well as lignans (25), polyacetylene (26) and polysaccharides (15).…”

Section: Introductionmentioning

confidence: 99%

Coumarins from the roots of Angelica dahurica cause anti-allergic inflammation

Liu

2017

Experimental and Therapeutic Medicine

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Abstract. Allergic inflammation is induced by allergens and leads to various allergic diseases, including rhinitis, asthma and conjunctivitis. Histamine is important in the pathogenesis of an immunoglobulin E-dependent allergic reaction and results in the secretion of cytokines associated with inflammation. Angelica dahurica (A. dahurica) is a medicinal plant widely used in China for the treatment of symptoms related to allergic inflammation. The present study investigated the chemical constituents from A. dahurica and evaluated their reductive effect on allergic inflammation. As a result, 15 compounds including 13 coumarins have been identified as isoimperatorin (1), imperatorin (2), oxypeucedanin (3), oxypeucedanin hydrate (4), bergapten (5), byakangelicin (6), phellopterin (7), byakangelicol (8), isopimpinellin (9), xanthotoxol (10), xanthotoxin (11), pimpinellin (12), scopoletin (13), β-sitosterol (14) and daucosterol (15). Compounds 1-13 were able to reduce the release of histamine, with compounds 4-6 exhibiting the most potent activity. Furthermore, compounds 1-12 were able to inhibit the secretion of tumor necrosis factor-α, interleukin (IL)-1β and IL-4, with compounds 5 and 7 exhibiting the strongest inhibitory effects. These compounds implemented the inhibitory effects on the expression of inflammatory cytokine genes through the inhibition of nuclear factor-κB activation. Virtual screening by a docking program indicated that compound 3 is a potent histamine H 1 receptor antagonist. Additionally, the calculated physicochemical properties of these compounds support most furanocoumarins to be delivered to binding sites and permeate the cell membrane. The present findings contribute to understanding how A. dahurica attenuates allergic inflammation.

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Section: Introductionmentioning

confidence: 99%

Coumarins from the roots of Angelica dahurica cause anti-allergic inflammation

Liu

2017

Experimental and Therapeutic Medicine

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“…[2][3][4][5] A large number of studies have shown that HO-1 played extensive protective roles in inflammatory diseases such as asthma, 4,[6][7][8][9] inflammatory bowel disease [10][11][12][13] and uveitis. 14,15 In addition, HO-1 exerts its effects on multiple immune cells, such as promoting maturation, differentiation and function of dendritic cells, 16,17 inhibiting the antigen-presenting function of dendritic cells, 18 inducing tolerance of dendritic cells 19 and suppressing degranulation of mast cells.…”

Section: Introductionmentioning

confidence: 99%

Heme oxygenase‐1 inhibits basophil maturation and activation but promotes its apoptosis in T helper type 2‐mediated allergic airway inflammation

Zhong¹,

et al. 2016

Immunology

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SummaryThe anti-inflammatory role of heme oxygenase-1 (HO-1) has been studied extensively in many disease models including asthma. Many cell types are anti-inflammatory targets of HO-1, such as dendritic cells and regulatory T cells. In contrast to previous reports that HO-1 had limited effects on basophils, which participate in T helper type 2 immune responses and antigeninduced allergic airway inflammation, we demonstrated in this study, for the first time, that the up-regulation of HO-1 significantly suppressed the maturation of mouse basophils, decreased the expression of CD40, CD80, MHC-II and activation marker CD200R on basophils, blocked DQ-ovalbumin uptake and promoted basophil apoptosis both in vitro and in vivo, leading to the inhibition of T helper type 2 polarization. These effects of HO-1 were mimicked by exogenous carbon monoxide, which is one of the catalytic products of HO-1. Furthermore, adoptive transfer of HO-1-modified basophils reduced ovalbumin-induced allergic airway inflammation. The above effects of HO-1 can be reversed by the HO-1 inhibitor Sn-protoporphyrin IX. Moreover, conditional depletion of basophils accompanying hemin treatment further attenuated airway inflammation compared with the hemin group, indicating that the protective role of HO-1 may involve multiple immune cells. Collectively, our findings demonstrated that HO-1 exerted its anti-inflammatory function through suppression of basophil maturation and activation, but promotion of basophil apoptosis, providing a possible novel therapeutic target in allergic asthma.

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“…Almolki et al (2004) reported that overexpression of HO has a notable protective effect and can decrease airway inflammation and hyperresponsiveness, mucus hypersecretion, and oxidative stress in a guinea pig model of allergic asthma. Lee et al (2011a) reported that administration of Angelica dahurica extract can suppress allergic airway inflammation induced by ovalbumin and suggested that it may be employed as an anti-inflammatory drug for the treatment of asthma. The mechanism behind this effect involves upregulation of HO-1.…”

Section: Discussionmentioning

confidence: 99%

Construction of a lentiviral vector encoding heme oxygenase 1 and its introduction into mouse adipose tissue-derived stem cells

Zhu

2015

Genet. Mol. Res.

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ABSTRACT. Many studies exist concerning the use of stem cells as delivery vehicles in gene therapy, expressing genes such as vascular endothelial growth factor 165 and hepatocyte growth factor. However, few reports regarding adipose tissue-derived stem cells (ADSCs) and the heme oxygenase 1 (HO-1) gene have been published. Therefore, we established a lentiviral vector encoding HO-1 and used this to infect ADSCs with the aim of producing therapeutic seed cells. In this study, ADSCs were isolated from mouse adipose tissue (AT), cultured, and identified according to the expression of antigens on their cell surface and their capacity for multilineage differentiation. A lentiviral vector encoding HO-1 was constructed, ADSCs were infected with this, and HO-1 protein expression was examined by western blotting. Our results show that ADSCs can be isolated from mouse AT, while DNA sequencing demonstrated that HO-1 was successfully transferred to the vector fused with GFP. Following 293T cell transfection, lentivirus titers were approximately 3 x 10 8 TU/mL. Fluorescence microscopy confirmed the expression of the HO-1 construct in lentivirus-infected ADSCs and the overexpression of HO-1 protein in these cells was verified by western blot. The production of ADSCs overexpressing HO-1 described in this study may aid in the development of a novel method for the treatment of asthma.

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Anti-asthmatic effects of Angelica dahurica against ovalbumin-induced airway inflammation via upregulation of heme oxygenase-1

Cited by 77 publications

References 41 publications

Coumarins from the roots of Angelica dahurica cause anti-allergic inflammation

Coumarins from the roots of Angelica dahurica cause anti-allergic inflammation

Heme oxygenase‐1 inhibits basophil maturation and activation but promotes its apoptosis in T helper type 2‐mediated allergic airway inflammation

Construction of a lentiviral vector encoding heme oxygenase 1 and its introduction into mouse adipose tissue-derived stem cells

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