Anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on retinal ganglion cells after optic nerve crush are PI3K/AKT-dependent

Tsai, Rong‐Kung; Chang, Chia-Wen; Sheu, Min-Muh; Huang, Zei-Lun

doi:10.1016/j.exer.2010.01.004

Cited by 50 publications

(56 citation statements)

References 41 publications

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“…The laser settings were the same as that reported in our previous paper. 14,27,28 Flash Visual-Evoked Potentials (FVEP)…”

Section: Raion Inductionmentioning

confidence: 99%

“…The details have been described in our previous reports. [26][27][28] Retinal Preparation. Briefly, the remaining eyecups containing the sclera and the retina were fixed in 4% paraformaldehyde for 2 hours at room temperature.…”

Section: On and Retinal Sample Preparationmentioning

confidence: 99%

“…[26][27][28] In brief, the retinas were examined for RGCs at a distance of 1 and 3 mm from the center to provide the central and midperipheral RGC densities, respectively. We counted at least eight randomly chosen areas in the central (approximately 40% of the central area) and midperipheral (approximately 30% of the midperiphery) regions of each retina, and their averages were used as the mean density of RGCs per retina (n ¼ 6 rats per group).…”

Section: Retrograde Labeling Of Rgcs With Fluorogold and Morphometry mentioning

confidence: 99%

“…The details have been described in our previous reports. 15,[26][27][28] Evans Blue (EB) Dye Extravasation of the ONs Optic nerve vascular leakage was quantified using modifications of the published techniques. 29,30 The details of the vascular permeability test have been described in our previous report.…”

Section: On and Retinal Sample Preparationmentioning

confidence: 99%

“…16,25,28 Briefly, we used a visual electrodiagnostic system (UTAS-E3000, LKC Technologies, Gaithersburg, MD, USA) to measure the FVEP. To exclude the possibility that the contralateral fellow eye contaminated the FVEP test, we covered the fellow eye while performing the stimulation.…”

Section: Raion Inductionmentioning

confidence: 99%

See 4 more Smart Citations

Early Methylprednisolone Treatment Can Stabilize the Blood-Optic Nerve Barrier in a Rat Model of Anterior Ischemic Optic Neuropathy (rAION)

Huang

Wen

Chang

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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“…The laser settings were the same as that reported in our previous paper. 14,27,28 Flash Visual-Evoked Potentials (FVEP)…”

Section: Raion Inductionmentioning

confidence: 99%

Section: On and Retinal Sample Preparationmentioning

confidence: 99%

Section: Retrograde Labeling Of Rgcs With Fluorogold and Morphometry mentioning

confidence: 99%

Section: On and Retinal Sample Preparationmentioning

confidence: 99%

Section: Raion Inductionmentioning

confidence: 99%

See 3 more Smart Citations

Early Methylprednisolone Treatment Can Stabilize the Blood-Optic Nerve Barrier in a Rat Model of Anterior Ischemic Optic Neuropathy (rAION)

Huang

Wen

Chang

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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Peritumoural adipose tissue pro‐inflammatory cytokines are associated with tumoural growth factors in cancer cachexia patients

Neto

Murari

Oyama

et al. 2018

J cachexia sarcopenia muscle

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BackgroundCancer cachexia (CC) is a multifactorial syndrome, often irreversible, that affects patients with cancer influenced, in part, by the inflammatory condition. Peritumoural adipose tissue produces adipokines and angiogenic, apoptotic, and growth factors; given the possible crosstalk between the peritumoural adipose tissue and tumour, these may play an important role in cancer biology and carcinogenesis.MethodsThe aim of this study was to evaluate the factors produced by peritumoural adipose tissue in a cohort of 16 colorectal cancer patients with either weight‐stable cancer (WSC; n = 7) or CC (n = 9). The study was approved by the Ethics Research Committee (972.914). Samples of peritumoural adipose tissue were analysed for concentrations of TNF‐α, IL‐1β, STAT‐1, STAT‐3, RANTES, IL‐1Ra, IP‐10, IL‐15, MCP‐1, IFN‐α, GCSF, FADD, and TGF‐β. The cytokines and proteins were measured using Multiplex. Correlations between the proteins and cytokines were evaluated.ResultsTNF‐α, STAT‐1, and FADD, a factor involved in apoptosis, were significantly higher in CC group than in the WSC group. In the peritumoural adipose tissue of the CC group, RANTES showed a significant positive correlation with IL‐1Ra and IP‐10 and a negative correlation with IFN‐α; and GCSF showed significant negative correlations with IL‐1Ra, IP‐10, IL‐15, and MCP‐1 and a positive correlation with IFN‐α. In the peritumoural adipose tissue of the WSC group, no significant correlations were detected between RANTES, GCSF, IL‐3, FADD, and STAT‐1 and the cytokines/chemokines analysed.ConclusionsThese results indicated that inflammatory and tumorigenic pathways were altered in peritumoural adipose tissue in CC. Furthermore, inflammatory cytokines were correlated with growth factors in the peritumoural adipose tissue of cachectic patients, suggesting that inflammatory cytokines modulated the proliferative environment closely linked to the tumour.

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Granulocyte macrophage colony‐stimulating factor promotes regeneration of retinal ganglion cells in vitro through a mammalian target of rapamycin‐dependent mechanism

Legacy

Hanea

Theoret

et al. 2013

J of Neuroscience Research

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Lack of regeneration in the adult central nervous system (CNS) is a major hurdle that limits recovery from neurological ailments. Although accumulating research suggests the possibility of axon regeneration by targeting intrinsic signaling mechanisms, it remains a matter of controversy whether functional recovery can be achieved by manipulating aspects of molecular signaling. Recent studies have shown that granulocyte macrophage colony-stimulating factor (GM-CSF) may be an effective means of targeting repair following CNS injury; how this molecule is able to produce this effect is not known. Indeed, GM-CSF has been shown to promote neuronal survival, potentially through activation of as yet unknown cytokine-dependent signals and potentially through regulation of antiapoptotic mechanisms. It is well established that the loss of intrinsic regenerative ability is highly correlated with development of CNS neurons. We therefore designed experiments, using a well-established in vitro retinal ganglion cell (RGC) culture system, to evaluate the effect of GM-CSF on axon growth and cell survival and define possible mechanisms involved in GM-CSF-mediated effects in vitro. Several developmental stages were evaluated, with particular focus placed on stages at which axon growth is known to be significantly diminished. Our results reveal that GM-CSF not only promotes axon growth in postnatal RGCs but also enhances cell survival through a mammalian target of rapamycin (mTOR)-dependent mechanism.

show abstract

Anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on retinal ganglion cells after optic nerve crush are PI3K/AKT-dependent

Cited by 50 publications

References 41 publications

Early Methylprednisolone Treatment Can Stabilize the Blood-Optic Nerve Barrier in a Rat Model of Anterior Ischemic Optic Neuropathy (rAION)

Early Methylprednisolone Treatment Can Stabilize the Blood-Optic Nerve Barrier in a Rat Model of Anterior Ischemic Optic Neuropathy (rAION)

Peritumoural adipose tissue pro‐inflammatory cytokines are associated with tumoural growth factors in cancer cachexia patients

Granulocyte macrophage colony‐stimulating factor promotes regeneration of retinal ganglion cells in vitro through a mammalian target of rapamycin‐dependent mechanism

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