Early Methylprednisolone Treatment Can Stabilize the Blood-Optic Nerve Barrier in a Rat Model of Anterior Ischemic Optic Neuropathy (rAION)

Huang, Tzu-Lun; Wen, Yao‐Tseng; Chang, Chung‐Hsing; Chang, Shu‐Wen; Lin, Kuan‐Hung; Tsai, Rong‐Kung

doi:10.1167/iovs.16-21017

Cited by 26 publications

(21 citation statements)

References 35 publications

(55 reference statements)

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“…This finding is consistent with previous reports that BONB stabilization can reduce inflammation and macrophage migration in ON tissue. 32 Furthermore, we demonstrated that P-sel-IgG exerts neuroprotection via the Nrf2 signaling pathway, consistent with a previous study that used a similar kind of PSGL-1 recombinant protein. 27 In addition, Nrf2 activation is protective in intracerebral hemorrhage 33 and middle cerebral artery occlusion in the brain, 34 which are also CNS disorders, along with AION.…”

Section: Discussionsupporting

confidence: 91%

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Soluble P-selectin promotes retinal ganglion cell survival through activation of Nrf2 signaling after ischemia injury

et al. 2017

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Retinal ischemic injuries play an important role in the pathogenesis of several eye disorders. Inflammation and oxidative stress are key players in ischemic injuries. Following retinal ischemia, vascular endothelial cells and leukocytes express several inflammatory adhesion receptors, such as selectins and cell adhesion molecules. P-selectin stimulates leukocyte recruitment to platelet aggregates and has an important role in vascular homeostasis and inflammatory leukocyte extravasation. Soluble P-selectin can be neuroprotective through competitive binding to the receptors of endogenous P-selectin molecules. Here, we demonstrate the neuroprotective effect of a recombinant P-selectin immunoglobin G (P-sel-IgG) chimeric fusion protein in a rat anterior ischemic optic neuropathy (rAION) model. rAION was induced by photodynamic therapy. P-sel-IgG treatment reduced optic nerve edema and stabilized the blood–optic nerve barrier (BONB) in the acute phase of rAION. Further, P-sel-IgG increased the retinal ganglion cell (RGC) survival rate, reduced RGC apoptosis, preserved visual function, maintained retinal nerve fiber layer thickness, and reduced macrophage infiltration in optic nerve tissue in the chronic phase (day 28). Increased NAD(P)H quinone dehydrogenase 1 (NQO1) and heme oxygenase 1(HO-1) expression levels, along with increased transcription factor Nrf2, suggesting an antioxidant role of P-sel-IgG via the Nrf2 signaling pathway. In conclusion, this study is the first to demonstrate that P-sel-IgG treatment promotes RGC survival by stabilizing the BONB and activating the Nrf2 signaling pathway in a rAION model.

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Section: Discussionsupporting

confidence: 91%

“…rAION causes endothelial cell damage 1 and increases vascular permeability. 32 Therefore, we decided to perform transmission electron microscopy (TEM) to study changes in ON tissue. Based on our OCT results ( Figure 5e ), we limited our study to ultrastructural changes in the acute phase (until day 7).…”

Section: Resultsmentioning

confidence: 99%

Soluble P-selectin promotes retinal ganglion cell survival through activation of Nrf2 signaling after ischemia injury

et al. 2017

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“…In our observations, treatment with astaxanthin decreased the levels of IL-1β and TNFα in the retina and reduced ED1-positive macrophage infiltration in the optic nerve after rAION induction. Our previous reports have demonstrated that the vascular permeability of the optic nerve was highly increased within two days post AION induction and that vascular-borne macrophages would infiltrate into ON [29,32]. Our results suggested that astaxanthin can halt the ED-1(+) cells infiltration into the ON.…”

Section: Discussionsupporting

confidence: 53%

“…Photoactivation of rose Bengal produces free radical oxygen in the optic nerve head (ONH), creating an oxidative stress environment followed by ischemic optic neuropathy and RGC death [29,39]. The detailed procedure is mentioned in our previous reports [32,39]. In brief, after intravenous injection of rose Bengal, the ONH was exposed with a fundus lens, and photoactivation of rose Bengal was performed using green argon laser on ONH.…”

Section: Animal Model and Ethics Statementmentioning

confidence: 99%

Haematococcus pluvialis-Derived Astaxanthin Is a Potential Neuroprotective Agent against Optic Nerve Ischemia

Lin

Kapupara²,

Wen³

et al. 2020

Marine Drugs

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Astaxanthin, a xanthophyll belonging to the family of carotenoids, is a potent antioxidant. However, much less is known about its protective effects on the oxidative stress of ischemic optic nerve. We hypothesized that astaxanthin treatment could protect retinal ganglion cells (RGCs) from death via anti-oxidative and anti-apoptotic responses. Adult male Wistar rats were fed astaxanthin (100 mg/kg/day) by daily gavage for seven consecutive days, either before or after inducing oxidative stress in the retina by photodynamic treatment. The visual function, RGC apoptosis, macrophage infiltration in the optic nerve, expression of p-Akt, p-mTOR, SGK1, pS6K, Nrf2, p62, TNFα, Il1β in retinas were investigated. The visual function and the RGC densities were significantly higher in both pre- and post-treatment groups. The numbers of apoptotic RGCs and extrinsic macrophage infiltration in the optic nerve were significantly decreased in both astaxanthin-treated groups. Furthermore, pre- and post-treatment of astaxanthin showed a higher expression of p-Akt, p-mTOR, Nrf2 and superoxide dismutase activity, and a lower expression of cleaved caspase-3, suggesting anti-apoptotic and anti-oxidative roles. Our findings indicate that astaxanthin can preserve visual function and reduce RGC apoptosis after ischemic insults. Including astaxanthin in daily diet as a supplement may be beneficiary for ischemic optic neuropathy.

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“…Die Blut-Hirn-Schranke wurde stabilisiert und die Makrophageninvasion erheblich reduziert. Damit sanken auch die Spiegel proinflammatorischen Cytokine wie TNF-α oder Interleukin-1β [21].…”

Section: Steroide?unclassified

Pathogenese und Therapie der nicht arteriitischen anterioren ischämischen Optikusneuropathie

Oterendorp

Lagrèze

Feltgen

2019

Klin Monatsbl Augenheilkd

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ZusammenfassungDie nicht arteriitische anteriore ischämische Optikusneuropathie (NAION) ist eine akute Sehnervendegeneration, deren Pathogenese in großen Teilen unerforscht ist. Für sie existiert derzeit keine allgemein akzeptierte Behandlungsmöglichkeit. Die Etablierung von AION-Tiermodellen sowie die Ergebnisse größerer epidemiologischer Studien ermöglichten es in den vergangenen Jahren, ein besseres Verständnis der Pathophysiologie und möglicher Einflussfaktoren zu gewinnen. Basierend auf diesen Erkenntnissen testeten und testen klinische und tierexperimentelle Studien eine große Bandbreite möglicher Behandlungsansätze. Unabhängig von ihrem jeweiligen Ergebnis können diese Untersuchungen unser Verständnis der NAION erheblich erweitern und auch für andere Sehnervendegenerationen von großer Bedeutung sein.

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Early Methylprednisolone Treatment Can Stabilize the Blood-Optic Nerve Barrier in a Rat Model of Anterior Ischemic Optic Neuropathy (rAION)

Cited by 26 publications

References 35 publications

Soluble P-selectin promotes retinal ganglion cell survival through activation of Nrf2 signaling after ischemia injury

Soluble P-selectin promotes retinal ganglion cell survival through activation of Nrf2 signaling after ischemia injury

Haematococcus pluvialis-Derived Astaxanthin Is a Potential Neuroprotective Agent against Optic Nerve Ischemia

Pathogenese und Therapie der nicht arteriitischen anterioren ischämischen Optikusneuropathie

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