Anti-angiogenic effects of water extract of a formula consisting of Pulsatilla koreana, Panax ginseng and Glycyrrhiza uralensis

Kim, Jong Min; Kim, Kyung-Soon; Lee, Yeon‐Weol; Cho, Chong-Kwan; Yoo, Hwa‐Seung; Bang, Ji‐Young; Kim, Eung-Yoon; Kang, In‐Cheol

doi:10.3736/jcim20110912

Cited by 9 publications

(5 citation statements)

References 24 publications

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“…In addition, Lico-A, the major bioactive compound isolated from Glycyrrhiza sp., has been reported to have various biological activities such as anti-inflammatory (31,32), anti-microbial (33), anti-angiogenic (34), anti-obesity (35) and osteogenic effects (4). In the present study, we demonstrated that Lico-A suppressed the proliferation and induced the apoptosis of KB oral cancer cells via death receptor-mediated caspase activation.…”

Section: Discussionmentioning

confidence: 99%

Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway

et al. 2013

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Licochalcone A (Lico-A) is a natural phenol licorice compound with multiple bioactivities, including anti-inflammatory, anti-microbial, anti-fungal and osteogenesis-inducing properties. In the present study, we investigated the Lico-A-induced apoptotic effects and examined the associated apoptosis pathway in KB human oral cancer cells. Lico-A decreased the number of viable KB oral cancer cells. However, Lico-A did not have an effect on primary normal human oral keratinocytes. In addition, the IC50 value of Lico-A was determined to be ~50 μM following dose-dependent stimulation. KB oral cancer cells stimulated with Lico-A for 24 h showed chromatin condensation by DAPI staining, genomic DNA fragmentation by agarose gel electrophoresis and a gradually increased apoptotic cell population by FACS analysis. These data suggest that Lico-A induces apoptosis in KB oral cancer cells. Additionally, Lico-A-induced apoptosis in KB oral cancer cells was mediated by the expression of factor associated suicide ligand (FasL) and activated caspase-8 and −3 and poly(ADP-ribose) polymerase (PARP). Furthermore, in the KB oral cancer cells co-stimulation with a caspase inhibitor (Z-VAD-fmk) and Lico-A significantly abolished the apoptotic phenomena. Our findings demonstrated that Lico-A-induced apoptosis in KB oral cancer cells involves the extrinsic apoptotic signaling pathway, which involves a caspase-dependent FasL-mediated death receptor pathway. Our data suggest that Lico-A be developed as a chemotherapeutic agent for the management of oral cancer.

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Section: Discussionmentioning

confidence: 99%

Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway

et al. 2013

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“…Angiogenesis of anaplastic thyroid cancer was also suppressed by Pulsatilla koreana extract [ 86 ]. The water extract of Pulsatilla koreana , Panax ginseng (Ren Shen in TCM) and Glycyrrhiza uralensis (Gan Cao in TCM) (WEPPG) significantly inhibited fibroblast growth factor-induced HUVEC proliferation, adhesion, migration, and capillary tube formation [ 146 ]. The protein levels of cyclin A (CCNA), TP63 and CDKN1C (also known as p27KIP2) were upregulated, while nibrin and focal adhesion kinase (FAK) were downregulated [ 146 ].…”

Section: Anti-angiogenic and Anti-metastatic Effectsmentioning

confidence: 99%

“…The blood vessel formation in a CAM treated with WEPPG was markedly reduced. WEPPG might exert its anticancer effects via the inhibition of angiogenesis [ 146 ].…”

Section: Anti-angiogenic and Anti-metastatic Effectsmentioning

confidence: 99%

Anticancer Chemodiversity of Ranunculaceae Medicinal Plants: Molecular Mechanisms and Functions

Hao

Shen³

et al. 2016

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The buttercup family, Ranunculaceae, comprising more than 2,200 species in at least 62 genera, mostly herbs, has long been used in folk medicine and worldwide ethnomedicine since the beginning of human civilization. Various medicinal phytometabolites have been found in Ranunculaceae plants, many of which, such as alkaloids, terpenoids, saponins, and polysaccharides, have shown anti-cancer activities in vitro and in vivo. Most concerns have been raised for two epiphany molecules, the monoterpene thymoquinone and the isoquinoline alkaloid berberine. At least 17 genera have been enriched with anti-cancer phytometabolites. Some Ranunculaceae phytometabolites induce the cell cycle arrest and apoptosis of cancer cells or enhance immune activities, while others inhibit the proliferation, invasion, angiogenesis, and metastasis, or reverse the multi-drug resistance of cancer cells thereby regulating all known hallmarks of cancer. These phytometabolites could exert their anti-cancer activities via multiple signaling pathways. In addition, absorption, distribution, metabolism, and excretion/toxicity properties and structure/activity relationships of some phytometabolites have been revealed assisting in the early drug discovery and development pipelines. However, a comprehensive review of the molecular mechanisms and functions of Ranunculaceae anti-cancer phytometabolites is lacking. Here, we summarize the recent progress of the anti-cancer chemo- and pharmacological diversity of Ranunculaceae medicinal plants, focusing on the emerging molecular machineries and functions of anti-cancer phytometabolites. Gene expression profiling and relevant omics platforms (e.g. genomics, transcriptomics, proteomics, and metabolomics) could reveal differential effects of phytometabolites on the phenotypically heterogeneous cancer cells.

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“…WEPPG is a water extract of a formula consisting of pulsatilla koreana, Panax ginseng, and glycyrrhiza uralensis, which significantly inhibited b-FGF-induced HUVEC proliferation, adhesion, migration, and capillary tube formation, ultimately, through inhibition of angiogenesis leads to anticancer effects (J. Kim et al, 2011).…”

Section: Anti-angiogenesismentioning

confidence: 99%

“…In VEGF or b‐FGF‐induced HUVEC cell models or mouse models, 20(R)‐Rg3 and 20(S)‐PPD/PPT had shown the different effects of either inhibition of angiogenesis or anti‐angiogenesis (Usami et al, 2008; Yue et al, 2006). WEPPG is a water extract of a formula consisting of pulsatilla koreana , Panax ginseng , and glycyrrhiza uralensis , which significantly inhibited b‐FGF‐induced HUVEC proliferation, adhesion, migration, and capillary tube formation, ultimately, through inhibition of angiogenesis leads to anticancer effects (J. Kim et al, 2011).…”

Section: Recent Findings Of Ginseng In the Treatment Of Vascular Dysf...mentioning

confidence: 99%

Ginseng in vascular dysfunction: A review of therapeutic potentials and molecular mechanisms

Zhang

Xue

et al. 2022

Phytotherapy Research

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Vascular dysfunction can lead to a variety of fatal diseases, including cardiovascular and cerebrovascular diseases, metabolic syndrome, and cancer. Although a large number of studies have reported the therapeutic effects of natural compounds on vascular-related diseases, ginseng is still the focus of research. Ginseng and its active substances have bioactive effects against different diseases with vascular dysfunction. In this review, we summarized the key molecular mechanisms and signaling pathways of ginseng, its different active ingredients or formula in the prevention and treatment of vascular-related diseases, including cardiac-cerebral vascular diseases, hypertension, diabetes complications, and cancer. Moreover, the bidirectional roles of ginseng in promoting or inhibiting angiogenesis have been highlighted. We systematically teased out the relationship between ginseng and vascular dysfunction, which could provide a basis for the clinical application of ginseng in the future.

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Anti-angiogenic effects of water extract of a formula consisting of Pulsatilla koreana, Panax ginseng and Glycyrrhiza uralensis

Cited by 9 publications

References 24 publications

Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway

Licochalcone A induces apoptosis in KB human oral cancer cells via a caspase-dependent FasL signaling pathway

Anticancer Chemodiversity of Ranunculaceae Medicinal Plants: Molecular Mechanisms and Functions

Ginseng in vascular dysfunction: A review of therapeutic potentials and molecular mechanisms

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