Anti-Angiogenic and Anti Oxidant Properties of Orthosiphon stamineus Benth. Methanolic Leaves Extract

Sahib, Hayder B; Aisha, Abdalrahim F. A.; Yam, Mun Fei; Asmawi, Mohd Zaini; Ismail, Zakiah; Salhimi, Salizawati Muhamad; Othman, N. H.; Majid, Amin Malik Shah Abdul

doi:10.3923/ijp.2009.162.167

Cited by 22 publications

(16 citation statements)

References 14 publications

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“…Due to the strong inhibition of VEGFR1 by eupatorin in vitro and the fact that some flavonoids structurally close to eupatorin as well as extracts from O. stamineus limit this process [10,34,35], we tested the antiangiogenic properties of eupatorin. We used HUVEC cells for modeling of angiogenesis in vitro and examined their ability to migrate and to form tubes in the presence of the compound.…”

Section: Eupatorin Inhibits Angiogenesis In Vitromentioning

confidence: 99%

Antiproliferative and antiangiogenic effects of flavone eupatorin, an active constituent of chloroform extract of Orthosiphon stamineus leaves

et al. 2012

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Section: Eupatorin Inhibits Angiogenesis In Vitromentioning

confidence: 99%

Antiproliferative and antiangiogenic effects of flavone eupatorin, an active constituent of chloroform extract of Orthosiphon stamineus leaves

et al. 2012

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“…extract inhibits oral cancer cells on human [2]. It supported by another research states that the methanol extract of this plant has a capacity to increase tamoxifen effect on breast cancer cell (MCF 7) proliferation [3].…”

Section: Drug Development To Obtain This Ideal Drug Is In Progress Tmentioning

confidence: 99%

Sinensetin-Rich Fraction Solid Dispersion Inhibits Cancer Cell Cycle

Arifianti¹,

Sukardiman²,

Santosa³

2017

KLS

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Drug development efforts to find new selective and safe drugs for cancer from natural resources are promising ones. The natural products are obtained in the multiple or single compounds. One of them is a sinensetin found in ethanol extract of Orthosiphon stamineus Benth. Sinensetin could inhibit cancer cell proliferation. However, it has a poor solubility so the absorption is low and then it impacts on the low bioavailability. The solubility problem in conventional drug could be solved by pharmaceutical manipulation. In the previous research, the manipulation was tried although there was no single compound found in the material tested. We found an optimal formula of a manipulation using solid disperse system of polyethylene glycol (PEG 6000) 15 times higher than sinensetin weight. This research was focused on observing the effect of the optimal formula of solid disperse system to inhibit cancer cell cycle. The cell lines used were T47D cells. The method used was flow cytometry. The result showed that the optimum formula has a consistent effect on the concentration of 40 and 60µg/mL. The sinensetin increase cell accumulation on S phase at the percentage of 18.80% (40µg/mL) and 22.21% (60µg/mL) compared to T47D normal cells. It reflects the S phase as the longest time experienced by the cells. Inhibition on S phase (S arrest) resulted from a DNA elongation. It causes an inhibition of DNA synthesis process. It could be concluded that the solid disperse of sinensetin was active to inhibit cancer cells proliferation on phase S. Keywords: cell cycle; sinensetin; solid disperse; poor solubility

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“…Angiogenesis is the formation of a mature blood vessel network through expansion and remodeling of the pre-existing vascular primordium. It plays a crucial role in the development of cancer [8,9]. In addition, sinensetin has chemo-sensitizing effect that can reverse P-glycoprotein as mediated multidrug resistance (MDR) by increasing the intracellular accumulation of drugs [10].…”

Section: Introductionmentioning

confidence: 99%

ENHANCEMENT AND OPTIMIZATION OF SINENSETIN EXTRACT FROM Orthosiphon stamineus USING SUPERCRTITICAL CARBON DIOXIDE EXTRACTION

Aziz¹,

Yunus²,

Lee³

et al. 2018

MJAS

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Sinensetin is a rare valuable flavonoid compound that contains 5 methoxy groups attached on the flavone backbone. This compound is abundantly established in citrus peel and can also be found in medicinal herb, 'misai kucing'. Scientifically, 'misai kucing' is known as Orthosiphon stamineus; mostly used to treat kidney stones by old folks. Orthosiphon stamineus was listed in National Key Economic Area (NKEA) as contributor towards the Malaysian Economic Growth. Thus, this study was conducted with objectives to determine suitable modifier for supercritical carbon dioxide (SC-CO 2 ) extraction and to optimize the SC-CO 2 extraction condition (pressure, temperature and average particle size) on the concentration of sinensetin. Water, methanol and acetone were used on the determination of modifier for SC-CO 2 extraction by using water bath extraction at 40 °C in 8 hours. The results showed that acetone gave the highest percentage of sinensetin extract of 0.11%. Therefore, acetone was used as a modifier to enhance the extraction of sinensetin. The concentration of sinensetin was increased and the highest percentage of sinensetin obtained was 6.99%. Response surface methodology (RSM) through Box-Behnken design via Design Expert Software was applied to optimize the SC-CO 2 conditions. The first-order polynomial was used to express the percentage of sinensetin with R 2 value of 0.542. The optimum SC-CO 2 operating condition was 30 MPa, 40 °C and 308.24 µm with sinensetin percentage of 4.35%. Hence, SC-CO 2 with small amount of modifier was the best method to extract sinensetin as compared to water bath extraction method. AbstrakSinensetin merupakan sebatian flavonoid yang jarang ditemui dan sangat berharga terdiri daripada 5 kumpulan metoksi yang terikat pada struktur flavone. Sebatian ini banyak dijumpai dalam kulit limau dan juga boleh didapati dalam herba 'misai kucing'. Secara saintifik, 'misai kucing' dikenali sebagai Orthosiphon stamineus; di mana 'misai kucing' digunakan untuk merawat batu karang oleh orang-orang tua. Orthosiphon stamineus telah disenaraikan dalam Kawasan Ekonomi Utama Negara (NKEA) sebagai penyumbang kepada pertumbuhan ekonomi negara. Oleh itu, kajian ini dijalankan dengan objektif untuk menentukan pelarut tambahan yang sesuai untuk pengekstrakan bendalir supergenting karbon dioksida (SC-CO 2 ) dan mengoptimumkan ISSN -2506 Ahmad Hazim et al: ENHANCEMENT AND OPTIMIZATION OF SINENSETIN EXTRACT FROMOrthosiphon stamineus USING SUPERCRTITICAL CARBON DIOXIDE EXTRACTION 868 pemboleh ubah SC-CO 2 (tekanan, suhu dan purata saiz zarah) terhadap kepekatan sinensetin. Air, metanol dan aseton telah digunakan untuk menentukan pelarut tambahan untuk pengekstrakan SC-CO 2 dengan menggunakan pengekstrakan rendaman air pada suhu 40 °C dalam masa 8 jam. Keputusan menunjukkan bahawa aseton memberikan peratusan sinensetin tertinggi sebanyak 0.11%. Oleh itu, aseton digunakan sebagai pelarut tambahan untuk meningkatkan pengekstrakan sinensetin dalam SC-CO 2 . Kepekatan sinensetin meningkat dan peratusan tertinggi sinen...

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Anti-Angiogenic and Anti Oxidant Properties of Orthosiphon stamineus Benth. Methanolic Leaves Extract

Cited by 22 publications

References 14 publications

Antiproliferative and antiangiogenic effects of flavone eupatorin, an active constituent of chloroform extract of Orthosiphon stamineus leaves

Antiproliferative and antiangiogenic effects of flavone eupatorin, an active constituent of chloroform extract of Orthosiphon stamineus leaves

Sinensetin-Rich Fraction Solid Dispersion Inhibits Cancer Cell Cycle

ENHANCEMENT AND OPTIMIZATION OF SINENSETIN EXTRACT FROM Orthosiphon stamineus USING SUPERCRTITICAL CARBON DIOXIDE EXTRACTION

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