Anti-angiogenic and anti-inflammatory effects of long-circulating liposomes co-encapsulating curcumin and doxorubicin on C26 murine colon cancer cells

Sesărman, Alina; Tefas, Lucia Ruxandra; Sylvester, Bianca; Licărete, Emilia; Rauca, Valentin Florian; Lupuţ, Lavinia; Pătraș, Laura; Banciu, Manuela; Porfire, Alina

doi:10.1016/j.pharep.2017.10.004

Cited by 69 publications

(31 citation statements)

References 32 publications

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“…Liposomal curcumin was prepared as PEG-coated nanoliposomes using the film hydration method, as previously described. 23 Briefly, the phospholipids were used in a concentration of 70 mM, of which 66.5 mM DPPC, the main lipid, and 3.5 mM PEG-2000-DSPE, a PEG-derived phospholipid, to achieve long circulation properties and an increased therapeutic efficiency. Cholesterol was added to the lipid mixture in 10:1 phospholipids to cholesterol molar ratio.…”

Section: Preparation Of Liposomal Curcuminmentioning

confidence: 99%

The effect of intravenous administration of liposomal curcumin in addition to sumatriptan treatment in an experimental migraine model in rats

Bulboacă¹,

Bolboacă²,

Stănescu³

et al. 2018

IJN

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BackgroundCurcumin has antioxidative properties that could be useful in various diseases due to its ability to act on multiple targets of various cellular pathways. We aimed to assess the efficacy of liposomal curcumin compared with curcumin solution, when in addition to sumatriptan (ST) treatment, in an experimental migraine model induced with nitroglycerin (NTG) in rats.MethodsSeven groups of 9 rats each were investigated: control group without migraine (1 mL saline solution intraperitoneal injection [ip]), control group with induced migraine, NTG+ST group (ST), NTG+ST+curcumin1 (CC1) group – 1 mg/100 g body weight (bw), NTG+ST+CC2 – 2 mg/100 g bw, NTG+ST+liposomal curcumin1 (lCC1) group – 1 mg/100 g bw, and NTG+ST+lCC2 (lCC2) group – 2 mg/100 g bw. NTG and ST were administered as 1 mL ip NTG | 1 mg/100 g bw and 1 mL ip ST | 1 mg/100 g bw, respectively. Plasma total oxidative stress (TOS), malondialdehyde (MDA), nitric oxide (NOx), thiol levels, as well as total antioxidative capacity (TAC) were assessed. The nociception process was assessed by counting the number of flinches and shakes after the formalin test.ResultsThe plasma TOS, MDA, and NOx levels, as oxidative stress parameters, were significantly decreased in the curcumin-treated groups, especially where curcumin was in liposomal form. The thiol and TAC were also improved by the curcumin treatment, with the best results obtained for the liposomal curcumin. The closest number of flinches and shakes to the control group was obtained for the group treated with liposomal curcumin at a dose of 2 mg/100 g bw.ConclusionLiposomal curcumin in a dose of 2 mg/100 g bw when in addition to ST treatment could be an optimum therapeutic strategy for migraine attacks and could represent a base for future clinical research and application.

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Section: Preparation Of Liposomal Curcuminmentioning

confidence: 99%

The effect of intravenous administration of liposomal curcumin in addition to sumatriptan treatment in an experimental migraine model in rats

Bulboacă¹,

Bolboacă²,

Stănescu³

et al. 2018

IJN

Self Cite

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“…Thus, the release of IL-1ra by POS-stimulated may help to protect the synthetic metabolic environment of the natural cartilage during bone cartilage repair. POS activating STAT-1 and -3 signaling pathways will not be beneficial to CC control while the increase of anti-inflammatory cytokines will result in the prevention of CC ( 37 , 92 ).…”

Section: Pos Affect Mitogen-activated Protein Kinases (Mapk) Signalinmentioning

confidence: 99%

Pectin Oligosaccharides Ameliorate Colon Cancer by Regulating Oxidative Stress- and Inflammation-Activated Signaling Pathways

et al. 2018

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Colon cancer (CC) is the third common neoplasm worldwide, and it is still a big challenge for exploring new effective medicine for treating CC. Natural product promoting human health has become a hot topic and attracted many researchers recently. Pectin, a complex polysaccharide in plant cell wall, mainly consists of four major types of polysaccharides: homogalacturonan, xylogalacturonan, rhamnogalacturonan I and II, all of which can be degraded into various pectin oligosaccharides (POS) and may provide abundant resource for exploring potential anticancer drugs. POS have been regarded as a novel class of potential functional food with multiple health-promoting properties. POS have antibacterial activities against some aggressive and recurrent bacterial infection and exert beneficial immunomodulation for controlling CC risk. However, the molecular functional role of POS in the prevention of CC risk and progression remains doubtful. The review focuses on antioxidant and anti-inflammatory roles of POS for promoting human health by regulating some potential oxidative and inflammation-activated pathways, such as ATP-activated protein kinase (AMPK), nuclear factor erythroid-2-related factor-2 (Nrf2), and nuclear factor-κB (NF-κB) pathways. The activation of these signaling pathways increases the antioxidant and antiinflammatory activities, which will result in the apoptosis of CC cells or in the prevention of CC risk and progression. Thus, POS may inhibit CC development by affecting antioxidant and antiinflammatory signaling pathways AMPK, Nrf2, and NF-κB. However, POS also can activate signal transduction and transcriptional activator 1 and 3 signaling pathway, which will reduce antioxidant and anti-inflammatory properties and promote CC progression. Specific structural and structurally modified POS may be associated with their functions and should be deeply explored in the future. The present review paper lacks the important information for the linkage between the specific structure of POS and its function. To further explore the effects of prebiotic potential of POS and their derivatives on human immunomodulation in the prevention of CC, the specific POS with a certain degree of polymerization or purified polymers are highly demanded to be performed in clinical practice.

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“…Previous studies have reported that curcumin inhibits the activity of inducible NOS of intestinal tract. Curcumin can also inhibit the production of many proinflammatory cytokines by inhibiting the activity of inducible NOS, which can alleviate the inflammatory reaction of mice with multidrug resistance gene deficiency, alleviate the development of colitis induced by sodium dextran sulfate, and inhibit the production of many proinflammatory cytokines [6] . NO free radicals and superoxide anions were effectively eliminated, and oxidative stress and acute gastric injury were alleviated [7] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Curcumin on Gastric Emptying Induced by L-arginine in Mice

Liu¹,

Yu²,

Wang³

et al. 2018

ijSciences

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Objective To investigate the mechanism of curcumin administration on gastric emptying induced by L-arginine in mice. Methods Kunming male mice were divided into control group, curcumin group, L-arginine group and curcumin L-arginine combination group.There were nine mice in each group. The curcumin group and curcumin L-arginine combination group were treated with curcumin solution once a day for 15 days. From the 11th day onwards, the L-arginine group and the curcumin L-arginine combination group were given intragastric administration of L-arginine once a day for 5 days. The other groups were given solvent solution as control. The gastric emptying rate, NO content and nitric oxide synthase (NOS) activity in gastric tissue were measured after fasting for 24 hours. Results There was no significant difference between single curcumin group and the control group (P>0.05). But the gastric emptying rate in L-arginine group was significantly lower (P<0.01), the NO and the activity of NOS in stomach tissue was significantly higher (P<0.01) than that in the control group. The gastric emptying rate in curcumin L-arginine combination group was significantly higher (P<0.01), the NO content and the activity of NOS in gastric tissue was significantly higher (P<0.01) than that in L-arginine group. Conclusion curcumin can inhibit the production of no by inhibiting the NOS activity of gastric tissue, and then improve the gastric emptying of mice. Keywords: Curcumin, L-arginine, Gastric EmptyingCurcumin is a phenolic plant of the family Curcumae extracted from the rhizome of turmeric. It has many biological activities, such as cardiovascular protection and anti-tumor [1 ， 2] . Curcumin could significantly improve the gastrointestinal peristalsis induced by L-arginine [3] . However, the specific mechanism of curcumin was not clear.The L-arginine was the precursor of NO. NO as a non-cholinergic non-adrenergic neurotransmitter can be produced under the action of nitric oxide synthase (NOS) in vivo and plays an inhibitory role in gastrointestinal motility. The effect of curcumin on the production of NO was investigated by detecting the content of NO and the activity of NOS in gastric tissue of mice. Methods Animals and treatmentThirty-six healthy male Kunming mice were randomly divided into control group, curcumin group, L-arginine group and curcumin L-arginine combination group. Curcumin group and curcumin L-arginine combination group was given curcumin suspension 200mg/kg for 15days. The L-arginine group was treated with L-arginine on the 11th day of gavage.The other groups were given the same amount of solvent solution as the control. Gastric emptying rateAt the end of the experiment, the mice in each group fasted for 24 hours and drank freely. The mice were perfused with 0.8ml half fluid suspension of 80% mass fraction of Arabinol, then the mice were killed after 20 min. The gastric cardia and pylorus were ligated by caesarean section, and the total weight of stomach was removed. The appetizers were cut along the great ...

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Anti-angiogenic and anti-inflammatory effects of long-circulating liposomes co-encapsulating curcumin and doxorubicin on C26 murine colon cancer cells

Cited by 69 publications

References 32 publications

The effect of intravenous administration of liposomal curcumin in addition to sumatriptan treatment in an experimental migraine model in rats

The effect of intravenous administration of liposomal curcumin in addition to sumatriptan treatment in an experimental migraine model in rats

Pectin Oligosaccharides Ameliorate Colon Cancer by Regulating Oxidative Stress- and Inflammation-Activated Signaling Pathways

Effect of Curcumin on Gastric Emptying Induced by L-arginine in Mice

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