Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells

Abstract

The aim of the present study was to examine whether the intestine gastrointestinal (GI) digests of abalone [Haliotis discus hannai (H. discus hannai)] modulate inflammatory responses and to elucidate the mechanisms involved. The GI digests of the abalone intestines were fractionated into fractions I (>10 kDa), II (5-10 kDa) and Ⅲ (<5 kDa). Of the abalone intestine GI digests (AIGIDs), fraction Ⅲ inhibited the passive cutaneous anaphylaxis (PCA) reaction in mice. Subsequently, a bioactive peptide [abalone intestine GI digest peptide (AIGIDP)] isolated from fraction Ⅲ was determined to be 1175.2 Da, and the amino acid sequence was found to be PFNQGTFAS. We noted that the purified nonameric peptide (AIGIDP) attenuated the phorbol‑12‑myristate 13-acetate plus calcium ionophore A23187 (PMACI)-induced histamine release and the production of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in human mast cells (HMC-1 cells). In addition, we also noted that AIGIDP inhibited the PMACI‑induced activation of nuclear factor‑κB (NF-κB) by suppressing IκBα phosphorylation and that it suppressed the production of cytokines by decreasing the phosphorylation of JNK. The findings of our study indicate that AIGIDP exerts a modulatory, anti-allergic effect on mast cell-mediated inflammatory diseases.

Fish bone peptide promotes osteogenic differentiation of MC3T3‐E1 pre‐osteoblasts through upregulation of MAPKs and Smad pathways activated BMP‐2 receptor

“…According to the previous reports, bioactive peptides have widely been utilized to improve their functional and nutritional properties via enzymatic hydrolysis method. Bioactive peptides can act as potential metabolism regulator that it reported various bioactivities such as osteoblast differentiation, anti‐oxidant, glucose uptake, and anti‐allergic activities. In particular, Jung et al had reported that fish bone peptide could not only promote bone formation and mineralization but also induce higher calcium solubility owing to increased calcium absorption .…”

Anti-allergic Effects of a Hot Water Extract of <i>Stephania tetrandra</i> S. Moore in RBL-2H3 Cells and an Allergic Rhinitis Mouse Model

“…Moreover, WEx exhibited very low toxicity. Anti-allergic peptides have been previously reported, 2016), LDAVNR and MMLDF (Vo et al, 2014), PFNQGTFAS (Ko et al, 2016), and peptides derived from casein (Tanaka et al, 2012). Thus, we speculate that WEx peptides may exert antiallergic effects, and that WEx contains safe and water-soluble antiallergic compounds.…”

Hypoxic mast cells accelerate the proliferation, collagen accumulation and phenotypic alteration of human lung fibroblasts

“…Mast cells are derived from CD34-expressing hematopoietic stem cells and have traditionally been recognized as sentinel cells in allergic and non-allergic immune responses under physiological conditions (13-15). Of note, accumulating evidence demonstrates that mast cells are involved in vascular remodeling through secretion of proangiogenic proteases and interaction with endothelial and smooth muscle cells, and they may be responsible for the development of pulmonary hypertension (16,17).…”