Anti-allergic Effects of a Hot Water Extract of <i>Stephania tetrandra</i> S. Moore in RBL-2H3 Cells and an Allergic Rhinitis Mouse Model

Abstract

Abbreviations: allergic rhinitis (AR), β-hexosaminidase (β-hex), cyclanoline (Cyc), fangchinoline (Fan), hot water extract (HWEx), immunoglobulin E (IgE), L-histidine decarboxylase (HDC), rat basophilic leukemia cell line (RBL-2H3), tetrandrine (Tet), tyrosine (Tyr),We investigated the anti-allergic activities of a hot water extract of Stephania tetrandra S. Moore using RBL-2H3 cells and an allergic rhinitis mouse model. The degranulation levels were significantly lower in the water-solvent fraction (WEx) and 99% ethanol fraction (99% EtEx) extracted from S. tetrandra than in the control. However, the cell viability of 99% EtEx was significantly lower than the control. In an ovalbumin allergic rhinitis mouse model, the frequency of sneezing after the fifth nasal ovalbumin (OVA) challenge in the groups intragastrically administered WEx and 99% EtEx decreased significantly compared to the control, although this decrease was lost following the sixth or seventh challenge. Plasma levels of OVA-specific immunoglobulin E in the WEx and 99% EtEx groups were significantly lower than the control at the experimental end point. Thus, S. tetrandra was proposed to be an effective anti-allergic natural medicine.

Antiallergic activities of Japanese leaf burdock extract in a rat basophilic leukemia cell line

“…β‐Hexosaminidase (β‐hex) was used as an index of antiallergic activity (Passante & Frankish, 2009). The β‐hex assay was performed according to previously published methods (Asano et al., 2011; Maeta et al., 2017). Briefly, RBL‐2H3 cells, at a density of 1.0 × 10 6 cells/ml, were seeded in 96‐well plates containing 100 μl of medium.…”

“…Cell viability was measured using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay following the method described by Maeta et al. (2017). Briefly, the supernatant was removed after the β‐hex assay and 100 μl of 0.5 mg/ml MTT dissolved in medium without phenol red was added to each well.…”

Anti-allergic Effects of the Alkaline Hydrolysis of Rapeseed Cake in a Rat Basophilic Leukemia Cell Line (RBL-2H3)

“…Next, we fractioned AH-RCEx following a method previously reported (Itoh et al, 2012;Maeta et al, 2017). First, we re-extracted AH-RCEx from 5.0679 g of RCEx.…”

“…We used β-hex as index of degranulation (Passante and Frankish, 2009). The β-hex assay was performed using a previously published method (Maeta et al, 2017;Asano et al, 2011;Yamamoto et al, 2011). RBL-2H3 cells were seeded in a 96 well plate at a density of 1.0 × 10 6 cells/mL in 100 μL of medium, incubated and sensitized for 16-18 h at 37 ℃ and 5 % CO 2 with 0.3 μg/ mL mouse monoclonal anti-dinitrophenyl (DNP) IgE (Sigma-Aldrich, St. Louis, USA).…”