Anti-adipogenic effects in 3T3-L1 cells of acetone extracts and fractions from Styrax japonica fruit

Abstract

Reduction in reactive oxygen species (ROS) production facilitated by Styrax japonica (SJ) extracts and fractions for inhibition of obesity development through inhibition of adipogenesis was investigated. Treatment of cells with SJ extracts and fractions did not result in changes in cell viability. SJ extracts and fractions inhibited lipid accumulation in a dose-dependent manner during adipogenesis. Expressions of PPARγ and C/EBPα, key adipogenic markers, were decreased in cells treated with SJ extracts and fractions. Inhibitory effects of SJ extracts and fractions were the most powerful in the early stages of differentiation (days 0-2). Intracellular ROS production was decreased in cells treated with SJ extracts and fractions. SJ extracts and fractions can inhibit adipogenesis via reduced ROS levels, which involves down-regulation of adipogenic transcription factors.

Aged black garlic extract regulates lipid metabolism by inhibiting lipogenesis and promoting lipolysis in mature 3T3-L1 adipocytes

“…Oil Red O staining was performed using Kim's protocol [12] with minor modifications. The morphology of the cells was examined under an inverted microscope (ZEISS, Oberkochen, Germany) and the images were captured using a digital camera (Nikon, Tokyo, Japan).…”

Anti-Obesity Effects of Petasites japonicus (Meowi) Ethanol Extract on RAW 264.7 Macrophages and 3T3-L1 Adipocytes and Its Characterization of Polyphenolic Compounds

“…Consequently, the importance of a healthy diet has been strongly emphasized. For this reason, extensive research has been carried out on the functionality of foods [16,17]. Polyphenols have various health functions.…”