Anti adipogenic activity of Aegle marmelos Correa

Karmase, Aniket; Jagtap, Sneha; Bhutani, K. K.

doi:10.1016/j.phymed.2013.07.011

Cited by 31 publications

(17 citation statements)

References 12 publications

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“…DMSO (0.2%) solution was selected as the solvent that achieved a complete dissolution of extracts and maintained approximately 70% cell viability in accord with previous studies [42]. The anti-adipogenic effects of samples at these concentrations has been shown not to be due to the effect of DMSO [43]. All extracts demonstrated more than 70% viability at ≤ 2 mg/mL, apart from BE and EE which showed similar viability at ≤ 1.5 mg/mL.…”

Section: Discussionmentioning

confidence: 73%

Phenolic Compounds with Antioxidant Properties from Canola Meal Extracts Inhibit Adipogenesis

Hussain

Rehman

Luckett

et al. 2019

IJMS

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The extraction of phenolic compounds from canola meal produces functional health products and renders the canola meal a more digestible animal feed. The extracted phenolics may have novel bioactivity worth investigation. In this study, several solvents were evaluated for their ability to extract phenolic compounds from canola meal: water (WE) and various 80% organic solvent/water mixtures of methanol (ME), acetone (AE), ethanol (EE), butanol (BE), chloroform (CE) and hexane (HE). The in vitro antioxidant and anti-obesity properties of various extracts were investigated. Anti-obesity properties were studied using adipogenic differentiation inhibition of a murine mesenchymal stem cell line (C3H10T1/2) and a pancreatic lipase inhibition assay. AE, ME, and BE showed significant (p < 0.05) adipogenesis and pancreatic lipase inhibitory activities and may have more pharmacological properties. AE down-regulated the gene expression of the major adipogenic transcription factor, peroxisome proliferator-activated receptor gamma (PPARγ), correlating to phenolic content in a dose-dependent manner. The chemical characterization of AE revealed the presence of sinapic acid, ferulic acid, and kaempferol derivatives as main bioactive phenols.

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Section: Discussionmentioning

confidence: 73%

Phenolic Compounds with Antioxidant Properties from Canola Meal Extracts Inhibit Adipogenesis

Hussain

Rehman

Luckett

et al. 2019

IJMS

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“…The compounds isolated from the CH 2 Cl 2 extract of The CH 2 Cl 2 extract and the compounds isolated from it, including compounds 2, 3, 4 and 5, have been reported by us for their antiobesity activity in the lipolysis and anti-adipogenesis assay. The fingerprint of the CH 2 Cl 2 extract was similar to that of the MeOH extract containing the same marker components [9,10]. Therefore, the present method is useful to carry out the standardization of the active extracts.…”

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confidence: 56%

Quantification and Comparison of Extraction Methods for Alkaloids in Aegle marmelos Leaves by HPLC

Karmase

Prasanna

Rasabattula

et al. 2014

Natural Product Communications

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The leaves of Aegle marmelos are reported to contain multi-bioactive classes of compounds including coumarins, furanocoumarins and alkaloids. HPLC analysis of the crude extract was challenging due to low concentrations of the compounds in the leaves. Five compounds visible in the HPLC chromatogram were separated and identified by HPLC and further elaborated for quantification as marker compounds of A. marmelos leaves using a C 18 column with detection at 275 nm. A gradient mobile phase consisting of acetonitrile and water was used. The developed HPLC method showed good linearity (r 2 ≥0.994), high precision (RSD<5%), and good recovery (99.27-99.98%) of the compounds. The lowest detection limit was 5 ng and the method was found to be robust. All the validation parameters were within the permissible limits. Therefore, the developed method is accurate and reliable for the quality control of A. marmelos. This is the first report of extensive quantitative HPLC analysis of marker compounds in A. marmelos leaves and method validation.

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“…It is one of the most important plants of Ayurveda and traditional Indian medicine [11]. Other reports indicate that marmelosin present in Aegle marmelos has antidiabetic activity [13][14][15][16]. Marmelosin (Imperatorin) is freely soluble in ethyl acetate extract [14].…”

Section: Methodsmentioning

confidence: 99%

“…Marmelosin (Imperatorin) is freely soluble in ethyl acetate extract [14]. Aegle marmelos traditionally also known as bael (golden apple) in India, which is belonging to family Rutaceae and one of the most important plant in the Ayurveda [15]. Components present in the leaf of Aegle marmelos have the ability to inhibit aldose reductase and help in delaying the progression of diabetic cataract [14].…”

Section: Methodsmentioning

confidence: 99%

Effect Ofco-Administration of Emblica Officinalis and Aegle Marmelos Extracts for Antioxidant and Antidiabetic Activity

Jain

Jaiswal

2019

Int J Pharm Pharm Sci

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Objective: The objective of the present study was to develop an HPLC analytical method and to perform in vivo study of Emblica officinalis and Aegle marmelos extracts for antioxidant and antidiabetic activity. Methods: The phytochemical analyses, total phenolic content (TPC), TLC, DPPH assay were performed for freeze-dried Emblica officinalis fruits aqueous extract (EOFAE) and Aegle marmelos leaves ethyl acetate extract (AMLEAE). The active constituents present in both extracts were estimated by using HPLC system having Hibar® C18 column [250 x4.6 mm, 5 µm] and UV detector (264 nm). A gradient mobile phase (acetonitrile and water with 0.1% trifluoroacetic acid) was used at a flow rate of 0.8 ml/min. In vivo antioxidant, antidiabetic activity of both extracts was conducted on male albino Wistar rats for 21 d in streptozotocin-induced diabetic rats (42 rats; n=6). The antidiabetic activity was measured by blood glucose level and biochemical parameters i.e. total cholesterol, triglycerides and total protein. Oxidative stress was measured by antioxidant biomarkers i.e. SOD, GSH, lipid peroxidation by thiobarbituric acid reactive substances method on the liver of the experimental rat. Results: Tannins, saponins, carbohydrate, glycosides are found in EOFAE; coumarins and flavonoids are found in AMLEAE and quinones, phenols are present in both extracts. The values of TPC present in standard gallic acid, EOFAE and AMLEAE were found to be 485.7, 315.6, 300.7 mgGAE/g, respectively. Rf values obtained by TLC of EOFAE and AMLEAE were found to be 0.41 and 0.50, respectively. The values of % inhibition shown by EOFAE and AMLEAE in DPPH assay were found to be 97.8%±2 and 95.2%±2, respectively. The values of retention time of EOFAE and AMLEAE by HPLC analysis were found to be 4.59 and 5.28 min, respectively. Histopathological examination of the liver was revealed that low dose EOAM (containing of EOFAE 250+AMLEAE 250 mg/kg body weight) administered once a daily for 21 d showed significant activity (P˂0.001) with biochemical parameters and antioxidant biomarkers. Conclusion: The present study showed that the EOFAE and AMLEAE treated group III with (EOAM) low dose of 500 mg/kg body weight has potent antioxidant and antidiabetic activity.

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Anti adipogenic activity of Aegle marmelos Correa

Cited by 31 publications

References 12 publications

Phenolic Compounds with Antioxidant Properties from Canola Meal Extracts Inhibit Adipogenesis

Phenolic Compounds with Antioxidant Properties from Canola Meal Extracts Inhibit Adipogenesis

Quantification and Comparison of Extraction Methods for Alkaloids in Aegle marmelos Leaves by HPLC

Effect Ofco-Administration of Emblica Officinalis and Aegle Marmelos Extracts for Antioxidant and Antidiabetic Activity

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