Anti-acne vulgaris effect including skin barrier improvement and 5α-reductase inhibition by tellimagrandin I from Carpinus tschonoskii

Abstract

BackgroundCarpinus tschonoskii (CT) has been previously studied for various activities in the improvement of skin diseases. In the present study, we examined the in vitro anti-acne vulgaris (AV) effect of CT leaves (CTL) and tellimagrandin I (TI), one of the main ellagitannins from CT, including skin barrier improvement and 5α-reductase inhibitory activity.MethodsTo test the anti-AV activities of CTL and TI, firstly, anti-oxidative and anti-inflammatory activities including DPPH radical scavenging activity, nitric oxide (NO) inhibitory activity, and cytokines [interleukin (IL)-6 and IL-8] were tested. Skin barrier improvement experiments were tested using developing cornified envelope (CE) formation, and filaggrin mRNA expression level was determined by RT-PCR. The 5α-reductase inhibitory activity was determined by measuring the testosterone levels in rat liver microsomes.ResultsCTL and TI showed potent anti-oxidative activity and anti-inflammatory activities. Especially, the cytokine production inhibitory activities of TI were found to be similar to the positive control, epigallocatechin gallate (EGCG). CTL and TI enhanced the CE formation and filaggrin mRNA expression levels and showed potent activities compared to that in the positive control, 1.5 mM Ca2+. In additionally, CTL and TI showed 5α-reductase inhibitory activities in a dose-dependent manner.ConclusionThe results showed that CTL and TI inhibit AV endogenous factors such as 5α-reductase and inflammatory cytokines and affect exogenous factors such as developing skin barrier function (CE and filaggrin levels). Therefore, CTL and TI may be plant-derived agent, promising in the treatment of acne vulgaris.

Plant cell culture extract of Cirsium eriophorum with skin pore refiner activity by modulating sebum production and inflammatory response

“…In the present article, we showed that C. (Li, Zhang, Zeng, Yang, & Deng, 2011;Regueiro et al, 2014;Troise, Ferracane, Palermo, & Fogliano, 2014 and reducing the desquamation impairment, respectively (Yin, Hwang, & Lee, 2019;Zhang et al, 2016;Zouboulis, Dessinioti, Tsatsou, & Gollnick, 2017). Indeed, hyper-keratinization of the infundibulum contributes to pore enlargement, together with alterations in the thickness of the epidermis and a loss of dermis structural components, thus the role of kallikrein 5 in reducing skin desquamation represents a key issue when treating this type of dysfunctions (Murakami, Kawamura, Jie, & Tanno, 2006).…”

“…In this study we demonstrated that a water‐soluble extract derived from C. eriophorum cell cultures was able to inhibit 5α‐Reductase activity and increase Kallikrein 5 expression when tested on skin cells in vitro, suggesting its potential role in sebum regulation and skin stratum corneum exfoliation. Both 5α‐Reductase inhibitors and kallikrein 5 inducers have recently drawn much interest in Cosmetics and Dermatology, due to their activity in helping anti‐acne therapeutic drugs and reducing the desquamation impairment, respectively (Yin, Hwang, & Lee, 2019; Zhang et al, 2016; Zouboulis, Dessinioti, Tsatsou, & Gollnick, 2017). Indeed, hyper‐keratinization of the infundibulum contributes to pore enlargement, together with alterations in the thickness of the epidermis and a loss of dermis structural components, thus the role of kallikrein 5 in reducing skin desquamation represents a key issue when treating this type of dysfunctions (Murakami, Kawamura, Jie, & Tanno, 2006).…”

In Vitro and In Vivo Regulation of SRD5A mRNA Expression of Supercritical Carbon Dioxide Extract from Asparagus racemosus Willd. Root as Anti-Sebum and Pore-Minimizing Active Ingredients

“…Zinc has been reported to reduce sebum production in vivo and to inhibit SRD5A via non-competitive inhibition of testosterone binding to SRD5A and the decreasing formation of the NADPH cofactor [ 13 ]. Many herbal extracts could alleviate androgen-stimulated sebum production from potent SRD5A inhibitor activity [ 44 , 52 , 53 ]. Moreover, saw palmetto, sesame seeds, and argan oil, which have high contents of polyphenolic compounds, have been introduced for use in facial sebum control [ 1 ].…”

Anti-Acne Vulgaris Effects of Pedunculagin from the Leaves of Quercus mongolica by Anti-Inflammatory Activity and 5α-Reductase Inhibition

“…Thus, inhibition of 5α-reductase activity improves AV by reducing DHT [18]. Several studies reported that natural sources and its active components, mainly polyphenols improve AV by inhibition of 5α-reductase activity [19][20][21][22][23].…”