Anthracene dimer cross-linked, washing- and sterilization-free hydrogel films for multicellular spheroid generation

Abstract

Three-dimensional multicellular spheroids are better in vitro cell models than two-dimensional cell monolayers, however, their applications are limited by their difficult production. PHEMA hydrogel films with honeycomb-like wrinkling patterns have...

Microstructured soft devices for the growth and analysis of populations of homogenous multicellular tumor spheroids

“…Furthermore, photolithography produces mainly flat-bottomed cavities to host cells and the resulting spheroids, while rather homogeneous in size, turn out to be heterogeneous in shape and apparently not engaging all possible cell-to-cell contacts. Self-patterning surfaces have also been proposed for MTS culture [42] and, while interesting, they do not provide a direct method to modify the pattern, the shape, and the size of the cavities where cells are grown and the necessary materials could pose problems of biocompatibility. 3D bioprinting is undergoing furious development nowadays and it will certainly provide valuable methods to produce MTS.…”

Stimuli-responsive dynamic hydrogels: design, properties and tissue engineering applications

“…67,68 The [4+4] cycloaddition reaction is similar to [2+2]-cycloaddition, wherein exposure to 365 nm causes dimerization and exposure to short UV light (o280 nm) causes dissociation. [69][70][71][72] Red-shift photodimerization of anthracene was observed when the benzyl imine group was conjugated to anthracene, wherein blue light irradiation causes dimerization. 73 Other photo-addition reactions that include allyl sulfides and disulfide addition-recombination reactions, are not of high importance for hydrogels yet.…”