Anthocyanin production in callus cultures of roselle (Hibiscus sabdariffa L.)

Mizukami, Hajime; Tomita, Kaomi; Ohashi, Hiromu; Hiraoka, Noboru

doi:10.1007/bf00272755

Cited by 45 publications

(19 citation statements)

References 19 publications

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“…The highest frequency of callusing (91.11%) was recorded when leaf explants were cultured on MS medium fortified with 4.0 mg l -1 2,4-D, followed by 3.0 mg l -1 (82.22%), which was statistically non-significant (P B 0.05). The frequency of callusing in petal explants was significantly lower (77.24%) at 4.0 mg l -1 2,4-D. A similar trend was observed in generation of callus biomass in Oxalis reclinata (Makunga et al 1997) and in Hibiscus sabdariffa (Mazukami et al 1988). The differential ability of the two explants to induce callus at the same 2,4-D concentration could have been due to different levels of endogenous auxins present in these explants.…”

Section: Callus Inductionsupporting

confidence: 75%

Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels

Ram

Prasad

Kaur

et al. 2010

Plant Cell Tiss Organ Cult

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The influence of varied concentrations of sucrose and ammonical (NH 4 ? ) nitrogen on in vitro induction and expression of anthocyanin pigments from Rosa hybrida cv. 'Pusa Ajay' was investigated. Of two explants (petal and leaf discs) selected and cultured under two different conditions (light and dark), leaf discs were found to be most suitable for callus initiation. Profuse and early callus induction was observed when leaf discs of rose were cultured under total dark conditions on solid Murashige and Skoog (MS) medium supplemented with 4.0 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D). Early pigment initiation, enhancement and maximum anthocyanin production from calluses were recorded when leaf discs were cultured on Euphorbia millii (EM) medium supplemented with 7% sucrose compared with calluses cultured at 4% sucrose concentration under 16/8 h (light/dark) photoperiod regime. Reducing the concentration of NH 4 ? nitrogen in the solid MS medium led to slight improvement in anthocyanin production in rose leaf calluses.

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Section: Callus Inductionsupporting

confidence: 75%

Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels

Ram

Prasad

Kaur

et al. 2010

Plant Cell Tiss Organ Cult

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“…The concentrations of ammonium used in the medium were essential to produce anthocyanins in the callus culture of strawberry (Fragaria ananassa cv Shikinari) (Fujita et al 1981a) and in cell suspension culture of grape (Meyer and Staden 1995;Ozeki and Komamine 1985;Yoshihiro and Atsushi 1985). In addition, the decrease of ammonium concentrations used in the medium was a key to produce shikonin in the callus and cell suspension cultures of L. erythrorhizon (Mizukami et al 1988). 2, 4-D was found to block anthocyanin formation completely in the carrot cell suspension culture (Meyer and Staden 1995) and inhibit anthocyanin formation in the callus culture of Oxalis linearis (Ozeki and Komamine 1986).…”

Section: Anthocyanin Biosynthesis In Pap1 Transgenic Callimentioning

confidence: 98%

Development of tobacco callus cultures over expressing Arabidopsis PAP1/MYB75 transcription factor and characterization of anthocyanin biosynthesis

Zhou

Zeng

Shi

et al. 2008

Planta

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The Arabidopsis PAP1 gene (At1g56650) encodes the MYB75 transcription factor, which has been demonstrated to essentially regulate the biosynthesis of anthocyanins. Our previous study showed that ectopic expression of the PAP1 gene led to high pigmentation of anthocyanins in all tissues of transgenic tobacco plants. In order to understand the mechanisms of how PAP1 regulates anthocyanin biosynthesis and what can regulate the function of PAP1, we have established PAP1 transgenic tobacco callus cultures. Phenotypically different calli including anthocyanin-producing red and anthocyanin-free white calli lines were differentially induced from the same genotype of PAP1 transgenic plants. RT-PCR analysis showed that the expression of the PAP1 transgene was similar in the two types of calli, indicating that the transgenic red and white calli had differential responses to the regulation of PAP1. The growth of transgenic red calli followed a "sigmoid-like" curve in a 25-day callus culture period, during which the time course obviously impacted the profiles and the average levels of anthocyanins even though the expression of the PAP1 transgene was constitutive. A HPLC-UV-ESI-mass spectrum-based profiling characterized nine anthocyanin molecules (e.g., 595, 579 and 609 m/z) in the transgenic red calli over the course of the culture period. Cyanidin, pelargonidin, and peonidin were the major anthocyanidins identified by HPLC-mass spectrum analysis. We have demonstrated that dark, nitrogen nutrients, and auxin apparently affect the anthocyanin profiles in PAP1 transgenic callus cultures; and suggest that these cell cultures are an appropriate system to study the regulatory function of PAP1 on the anthocyanin biosynthesis at post-transcriptional level in vivo.

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“…Because of their low toxicity, anthocyanins have a high potential as a food additive and marker, so many institutes and food manufacturers are engaged in intensive research to produce these pigments from various plant cell cultures: Euphorbia millii (Yamamoto et al 1982), Callistephus chinensis (Rau & Forkman 1986), sweet potato (Nozue et al 1987), Centaurea cyanus (Kakegawa et al 1987), Hibiscus sabdariffa (Mizukami et al 1988), Perilla frutescens (Zhong et al 1991), etc. Anthocyanins, however, usually accumulate only in small amounts in cultured plant cells and their production generally requires light irradiation.…”

Section: Introductionmentioning

confidence: 99%

Anthocyanin production in cultured cells of Aralia cordata Thunb.

Sakamoto¹,

Iida²,

Sawamura³

et al. 1994

Plant Cell Tiss Organ Cult

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High anthocyanin-producing cell lines, which were grown in a dark or in a light-dark regime, were selected from callus cultures initiated from stem and leaf tissues of Aralia cordata Thunb. by small-cell-aggregate selection. To verify the optimum culture conditions for anthocyanin production, cells were tested by changing the various basal media, sucrose concentration and nitrogen source and concentration. Good growth was obtained in the dark on Linsmaier-Skoog's basal medium containing 1.0 mg 1-1 2,4-D and 0.1 mgl -~ kinetin, 2% (w/v) sucrose and full strength of nitrogen concentration. However, the highest anthocyanin yield (10.3% dry wt) was obtained in the dark on B5 medium containing 1.0 mg 1-1 2,4-D and 0.1 mg 1-1 kinetin. Our results suggested that it has became feasible to find the most effective conditions for cell growth and anthocyanin production by optimizations of the nitrogen concentration and the ratio of NH 4 to NO 3 in the medium.

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Anthocyanin production in callus cultures of roselle (Hibiscus sabdariffa L.)

Cited by 45 publications

References 19 publications

Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels

Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels

Development of tobacco callus cultures over expressing Arabidopsis PAP1/MYB75 transcription factor and characterization of anthocyanin biosynthesis

Anthocyanin production in cultured cells of Aralia cordata Thunb.

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