Anther culture in pepper (Capsicum annuum L.) in vitro

Irikova, Teodora; Grozeva, Stanislava; Родева, Величка

doi:10.1007/s11738-011-0736-6

Cited by 81 publications

(68 citation statements)

References 65 publications

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“…Anther cultures have been used in a number of cultivars with relative efficiency (reviewed in Irikova et al 2011;Seguí-Simarro et al 2011), but successful application of microspore cultures has only been reported in few hot (asian) pepper types (Kim et al 2008;Supena et al 2006b). Nevertheless, the stages most sensitive to inductive treatments in pepper revolve around the VM and YBP (Irikova et al 2011), exactly the same described for most of the known inducible species. Pepper is not an exception in this sense.…”

Section: Introductionmentioning

confidence: 99%

Morphological markers to correlate bud and anther development with microsporogenesis and microgametogenesis in pepper (Capsicum annuum L.)

2012

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The identification of microspores or pollen grains at particular developmental stages during micrporogenesis or microgamentogenesis is an important step for different basic and applied purposes. Among them, the most relevant example from a biotechnological perspective is the production of androgenic doubled haploids. For this and other techniques, precise, fast, easy and reliable criteria to identify flower buds carrying microspores or pollen at particular stages are essential. In anthocyaninproducing pepper types, the particularities of flower development allow for the identification of several morphological markers potentially useful as criteria for such an identification. In this work, our aim was to determine the easiest and more accurate criterion to correlate visible, measurable traits of bud and anther development with each of the individual stages of microsporogenesis and microgametogenesis. For this, we used three Spanish sweet pepper F1 hybrids ('Herminio', 'Gacela' and 'Águila'). We analyzed and discussed the accuracy and practical usefulness of using anther length, bud length, anther purple pigmentation and the ratio between calyx length and bud length (calyx/bud ratio) as predictors of individual microspore/pollen developmental stages.According to our results, we propose a combination of calyx/bud ratio and anther 1 pigmentation as an easy, fast and accurate criterion potentially applicable to anthocyanin-producing pepper cultivars to determine their particular markers.

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Section: Introductionmentioning

confidence: 99%

Morphological markers to correlate bud and anther development with microsporogenesis and microgametogenesis in pepper (Capsicum annuum L.)

2012

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“…The several reasons for this inefficiency in production of callus or embryo-like structures from anthers and subsequent plant regeneration include the genotypic dependence, collection of donor buds in the optimal stage, stress treatments as well as composition of medium and the culture conditions (Kothari et al, 2010 andIrikova et al, 2011).…”

Section: Issn: 2319-7706 Volume 6 Number 5 (2017) Pp 925-933mentioning

confidence: 99%

In vitro Androgenesis in Capsicum (Capsicum annuum L.)

Hegde¹,

Partap²,

Yadav³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…Despite the considerable number of studies conducted with pepper anther culture, the reported effectiveness of the process is still low (Irikova et al, 2011a). A high frequency of anther-derived embryos can be obtained, but few of them develop into normal plants (Segui-Simarro et al, 2011a).…”

Section: Introductionmentioning

confidence: 99%

Pepper (Capsicum annuum L.) anther culture: fundamentalresearch and practical applications

Irikova¹,

Kintzios²,

Grozeva³

et al. 2016

Turk J Biol

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IntroductionIn general, haploid cells or individuals are those in which the original chromosome number has reduced by half. According to Seguí-Simarro (2010), androgenesis can be defined as a set of biological processes leading to an individual that genetically originated exclusively from a male nucleus. After spontaneous or induced genome doubling, double haploids (DHs) that are completely homozygous are obtained. The production of haploid plants can be induced by methods such as parthenogenesis, interspecific hybridization, pollination with irradiated pollen, hormonal and/or chemical treatment, and application of high-temperature stress (Forster et al., 2007;Murovec and Bohanec, 2013). In anther culture, haploids and DHs occur through direct microspore embryogenesis. This technique has no analogue for fast creation of pure homozygous lines in a single step. In pepper-plant breeding, for example, this method has helped in the development of important new varieties with improved adaptability, productivity, and disease and pest tolerance (Chunling and Baojun, 1995;Venczel and Mitykó, 1995;Arnedo Andrés et al., 2004;Nervo et al., 2007). DH lines are a suitable material for genetic and molecular studies, and this determines their high value in molecular breeding as well (Madhusudhana, 2015).Regarding the genus Capsicum, the first haploids were derived by in vitro anther culture of C. annuum and C.

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Anther culture in pepper (Capsicum annuum L.) in vitro

Cited by 81 publications

References 65 publications

Morphological markers to correlate bud and anther development with microsporogenesis and microgametogenesis in pepper (Capsicum annuum L.)

Morphological markers to correlate bud and anther development with microsporogenesis and microgametogenesis in pepper (Capsicum annuum L.)

In vitro Androgenesis in Capsicum (Capsicum annuum L.)

Pepper (Capsicum annuum L.) anther culture: fundamentalresearch and practical applications

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