Anthelmintic resistance in Swedish sheep flocks based on a comparison of the results from the faecal egg count reduction test and resistant allele frequencies of the β-tubulin gene

Höglund, Johan; Gustafsson, Katarina; Ljungström, B.-L.; Engström, Annie; Donnan, Alison; Skuce, Philip

doi:10.1016/j.vetpar.2008.12.001

Cited by 82 publications

(68 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The prevalence of GSTT1 null allele in the present study ranges from 11% to 22%, which is almost similar to the frequencies reported in Caucasians (Blackburn et al, 2006;Johansson et al, 1998;Mannervik et al, 2005). Korean population showed higher frequency of (45.3%) of GSTT1 null allele compared with the white Americans (20.4%), African Americans (21.8%), Mexican-Americans (9.7%) (Hoglund et al, 2009;Marinkovic et al, 2008) and Turkish populations (10.8-28.3%) (Oke et al, 1998;Shchipanov et al, 2008;Sura et al, 2008). The GSTT1 null allele frequency in Native Russians is very close to allelic frequencies observed in some European populations (Baysal et al, 2008).…”

Section: Discussionsupporting

confidence: 86%

Gene Frequencies of the Human GSTT1 (Null Allele) and GSTP1 (Ile105Val) Polymorphisms among South Indian Populations

Lakkakula¹,

Maram²,

Gurramkonda³

et al. 2013

ACRT

View full text Add to dashboard Cite

Background: Glutathione S-transferases (GSTs) are members of the phase II biotransformation enzymes that play a key role in cellular detoxification of chemical carcinogens and xenobiotics. Variations at GST genes have been reported in different human populations, and some association studies have reported increased risk for cancers and other disease end points. The present study was conducted to investigate the allele frequency variations in south Indian populations.

show abstract

Section: Discussionsupporting

confidence: 86%

Gene Frequencies of the Human GSTT1 (Null Allele) and GSTP1 (Ile105Val) Polymorphisms among South Indian Populations

Lakkakula¹,

Maram²,

Gurramkonda³

et al. 2013

ACRT

View full text Add to dashboard Cite

show abstract

“…The importance of tubulin binding by benzimidazoles has been demonstrated by the finding that parasites that are resistant to these drugs have mutations in the β-tubulin gene (Kwa et al 1995, Höglund et al 2009, Lake et al 2009, Rufener et al 2009). In vitro studies of benzimidazole resistance have used a tubulin-binding assay as well as egg hatch assays (Johansen & Waller 1989, Martin et al 1989).…”

Section: Benzimidazolesmentioning

confidence: 99%

In vitro effect of seven antiparasitics on Acolpenteron ureteroecetes (Dactylogyridae) from largemouth bass Micropterus salmoides (Centrarchidae)

Reimschuessel¹,

Gieseker²,

Poynton³

2011

Dis. Aquat. Org.

View full text Add to dashboard Cite

Few drugs are approved by the United States Food and Drug Administration for treating parasite infections in minor species such as fish, due in part to the high cost of developing such drugs and to a relatively small market share for drug sponsors. Because in vivo effectiveness trials for antiparasitic drugs are costly, time consuming, and use many animals, a systematic in vitro screening approach to describe parasite motility could help find promising drug candidates. We evaluated the effects of 7 antiparasitics on the activity and survival of the endoparasitic monogenean Acolpenteron ureteroecetes (Dactylogyridae) collected from the posterior kidneys of juvenile largemouth bass Micropterus salmoides (Lacepede, 1802) (Centrarchidae) held in the laboratory. Tests were conducted in 12 well tissue culture plates; each well had 3 parasites, and we tested 3 concentrations and 1 control for each of the 7 antiparasitics. The parasites were observed immediately after adding the drug, at 1 to 3 h, and 17 to 26 h, and video recordings were made. Drug effects were recorded by documenting morbidity (reduced movement, tremors, contracted body, abnormal morphology) and mortality. A. ureteroecetes was strongly affected by the quinoline praziquantel, the imidazothiazide levamisole, and the organophosphates dichlorvos and trichlorfon. The parasites were moderately affected by the macrocyclic lactones ivermectin and emamectin, and generally unaffected by the benzimidazole mebendazole. Our study demonstrates the utility of characterizing in vitro responses with video microscopy to document responses of fish parasites for initial screens of drug effects on a fish monogenean.

show abstract

“…Control of these parasites has relied primarily on the intensive use of anthelmintic drugs such as benzimidazole drugs (BZ), primarily albendazole, oxfendazole, and fenbendazole; macrocyclic lactones such as ivermectin and imidazothiazole; and tetrahydropyrimidine derivatives such as levamisole and morantel-tartrate 3 . Nevertheless, with the continuous use of these anthelmintic drugs, anthelmintic resistance (AR) has developed in parasites [4][5][6][7][8][9][10] . In particular, the anthelmintic resistance AR of gastrointestinal nematodes (GIN) in sheep occurs globally and is described as a major threat to sheep production 11,12 .…”

Section: Introductionmentioning

confidence: 99%

“…For example, gastrointestinal nematodes were resistant to benzimidazole on all farms assessed with a previous history of benzimidazole use in ithuanian sheep 13 , and in many flocks in India (14) . Moreover, AR reaches high levels in Asia, Latin America, Africa, Australia, and New Zealand, in addition to regions that rely on small ruminants as one of the primary sources of animal protein [4][5][6][7][8][9][10] . In Mexico, low resistance to benzimidazole was demonstrated in southeastern Mexico in 2003, but the authors warned that resistance could increase due to the continuous use of benzimidazole derivatives 15 .…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic resistance status of gastrointestinal nematodes of sheep to the single or combined administration of benzimidazoles and closantel in three localities in Mexico

et al. 2016

View full text Add to dashboard Cite

Sheep production requires the constant assessment of parasitic burden and the efficacy of existing treatments for proper management. In this study, the administration of five different treatments was evaluated for the reduction of the percentage of eggs per gram of feces (EPG) shed by gastrointestinal nematodes (GIN) from sheep on three different sheep-breeding farms in Mexico (Texcoco, Estado de Mexico; Hueytamalco, Puebla; and Tlaltizapán de Zapata, Morelos). In these farms, ivermectin and benzimidazole derivatives had been routinely administered for two consecutive years. To determine whether drugs with different pharmacological properties decreased GIN fecal egg excretion, the treatments closantel (CLOS), albendazole (ABZ) and fenbendazole (FBZ) were administered alone and in combinations of CLOS + ABZ and CLOS + FBZ, to five groups of sheep, with an additional untreated control group on each farm (n = 28 per flock). Anthelmintic resistance was determined using Fecal Egg Count Reduction Tests (FECRT) as recommended in the guidelines of the World Association for the Advancement of Veterinary Parasitology. Fecal samples were collected 14 and 21 days after treatment. The anthelmintic resistance status was determined based on the reduction in the fecal egg count arithmetic mean and 95 % confidence limits. According to the FECRT, resistance developed to CLOS, ABZ, FBZ and CLOS + FBZ because the mean percentage of EPG reduction was ≤ 95 % with a lower confidence limit of ≤ 90 %. By contrast, nematode susceptibility was confirmed for the CLOS + ABZ combination, as it reduced the percentage of GIN fecal egg output by 96.46 ± 3.04 % (day 14) and 96.88 ± 3.04 % (day 21). Based on the morphometric identification of larvae, Haemonchus spp., Cooperia spp. and Teladorsagia spp. were the most abundant genera on all farms before the administration of these five treatments. In conclusion, the use of the anthelmintic combination of closantel plus albendazole may reduce the development of anthelmintic resistance in gastrointestinal nematodes.

show abstract

Anthelmintic resistance in Swedish sheep flocks based on a comparison of the results from the faecal egg count reduction test and resistant allele frequencies of the β-tubulin gene

Cited by 82 publications

References 17 publications

Gene Frequencies of the Human GSTT1 (Null Allele) and GSTP1 (Ile105Val) Polymorphisms among South Indian Populations

Gene Frequencies of the Human GSTT1 (Null Allele) and GSTP1 (Ile105Val) Polymorphisms among South Indian Populations

In vitro effect of seven antiparasitics on Acolpenteron ureteroecetes (Dactylogyridae) from largemouth bass Micropterus salmoides (Centrarchidae)

Anthelmintic resistance status of gastrointestinal nematodes of sheep to the single or combined administration of benzimidazoles and closantel in three localities in Mexico

Contact Info

Product

Resources

About