Anthelmintic resistance in gastrointestinal nematodes in goats and evaluation of FAMACHA diagnostic marker in Uganda

Nabukenya, Immaculate; Rubaire-Akiiki, Chris; Olila, Deogracious; Muhangi, Denis; Höglund, Johan

doi:10.1016/j.vetpar.2014.07.019

Cited by 32 publications

(33 citation statements)

References 28 publications

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“…The current study was the only test that had verified resistance to all four of the anthelmintics tested. This problem of resistance to multiple anthelmintics is becoming more evident globally (Byaruhanga et al, 2013;Nabukenya et al, 2014;Abubakar et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of parasite resistance to commonly used commercial anthelmintics in meat goats on humid subtropical pasture

Goolsby

Leite-Browning

Browning

2017

Small Ruminant Research

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Anthelmintic-resistant gastrointestinal nematode parasites are a threat to small ruminant industry sustainability. Meat goat does were administered one of four anthelmintics orally (ivermectin (n = 18), moxidectin (n = 18), levamisole hydrochloride (n = 17), or albendazole (n = 19)) or water (n = 18). Fecal samples were collected pretreatment and 12 days post-treatment. Fecal egg counts (FEC) were determined by the modified McMaster technique. The FEC reduction percentages (FECR%) were calculated using three equations. Log transformed FEC means were analyzed by treatment, sire breed of doe, and doe age. Sire breed affected (P < 0.05) pretreatment FEC, but not post-treatment FEC (P = 0.12). Pretreatment FEC did not differ (P = 0.21) by treatment group. Posttreatment FEC varied (P < 0.05) by treatment. Anthelmintic resistance determinations were based on FECR% falling below 90% or 80%, dependent on equation applied. Resistance was detected to all four anthelmintics using each equation. These results suggest the need for alternative methods of internal parasite control in goats.

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Section: Discussionmentioning

confidence: 99%

Evaluation of parasite resistance to commonly used commercial anthelmintics in meat goats on humid subtropical pasture

Goolsby

Leite-Browning

Browning

2017

Small Ruminant Research

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“…Penggunaan obat cacing yang berlebihan telah dilaporkan menyebabkan resistensi pada anak sapi dan ruminansia kecil (Garg et al 2007;Ram et al 2007). Saat ini telah banyak dilaporkan kejadian resistensi cacing nematoda terhadap obat cacing di beberapa negara (Papadopoulos 2008;Nabukenya et al 2014) termasuk Indonesia (Haryuningtyas & Beriajaya 2002). Sehingga untuk mengetahui efektivitas obat cacing terlebih dahulu dapat dilakukan uji in vitro efektivitas obat cacing terhadap telur, larva dan cacing dewasa.…”

Section: Pendahuluanunclassified

Media Penyimpanan Telur, Larva dan Cacing Nematode sebagai Media Uji In Vitro

Ekawasti

Suhardono

et al. 2017

Teknologi Peternakan Dan Veteriner Mendukung Diversifikasi Sumber Protein Asal Ternak

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Gastrointestinal nematode worm infection is one of the factors inhibiting efforts to improve livestock productivity. The treatment is done by anthelmintic, but lately have been several cases of drug resistance worm. So that needs to be tested to determine the effectiveness of worm medicine. The test of effectiveness of drugs against worms can be done in vitro either on eggs, larvae and adult worms to chemical anthelmintics and herbs anthelmintics. Before performing the in vitro test, first need to know a good media in vitro test for use to the egg, larva and nematode worms can survive well beyond its natural condition. This study was aimed to test the good solvent medium for the storage of eggs, larvae and adult worms that can be used in vitro tests. Solvent medium or the test medium in vitro as the storage of eggs, larvae and adult worms commonly used is a solution of isotonic or a solution containing a substance, such as 0.9% NaCl, DMSO 1%, PBS 1%, CMC 1%, Tween 20 1%, and distilled water. Eggs and larvae are stored in the petridish that already contains six media with each of the three repetitions and time of observation for 24 and 48 hours, while the adult worms was observed every hour for 5 hours. Result showed that in vitro medium good for eggs and larvae was DMSO 1% and PBS 1%, while for adult worms NaCl 0.9% and PBS 1%. PBS is a good medium if the test will be conducted simultaneously using eggs, larvae and adult nematode worms.Key Words: Nematodes, The Digestive Tract, Media, In vitro ABSTRAKInfeksi cacing nematoda saluran pencernaan merupakan salah satu faktor penghambat dalam meningkatkan produktivitas ternak. Pengobatannya dilakukan dengan pemberian obat cacing, tetapi akhir-akhir ini telah dilaporkan adanya kejadian resistensi obat cacing. Sehingga perlu dilakukan uji untuk mengetahui efektivitas obat cacing. Uji efektivitas terhadap obat cacing dapat dilakukan secara in vitro baik pada telur, larva dan cacing dewasa terhadap anthelmintik kimia maupun alami (herbal). Sebelum melakukan uji in vitro terlebih dahulu perlu diketahui media uji in vitro yang baik untuk digunakan agar telur, larva dan cacing nematoda dapat bertahan dengan baik di luar kondisi alamnya. Penelitian ini bertujuan untuk menguji media pelarut yang baik untuk penyimpanan telur, larva dan cacing dewasa agar dapat digunakan pada uji in vitro. Media pelarut atau media uji in vitro sebagai penyimpanan telur larva dan cacing dewasa yang biasa digunakan adalah berupa larutan isotonis atau larutan yang mengandung zat tertentu, seperti NaCl 0,9%, DMSO 1%, PBS 1%, CMC 1%, Tween 20 1%, dan akuades. Telur dan larva disimpan pada cawan petri yang telah berisi enam media tersebut dengan masing-masing 3 pengulangan dan waktu pengamatan selama 24 dan 48 jam sedangkan pada cacing dewasa dilakukan pengamatan tiap jam selama lima jam. Diperoleh hasil bahwa media in vitro yang baik untuk telur dan larva cacing adalah DMSO 1% dan PBS 1% sedangkan untuk cacing dewasa NaCl 0,9% dan PBS 1%. PBS merupakan media yang baik jika akan dilakukan uji ...

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“…infections caused by gastrointestinal nematodes in goats remain as a global challenge (JABBAR et al, 2006;NABUKENYA et al, 2014). These infections are responsible for a significant economic impact due to weight loss, reduced milk production and delayed growth (ROEBER et al, 2013).…”

Section: Parasiticmentioning

confidence: 99%

Ovicidal activity of succinic acid isolated from sisal waste (Agave sisalana) against gastrointestinal nematodes of goats

Santos

et al. 2017

Cienc. Rural

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This study was conducted to evaluate the in vitro anthelmintic activity of the succinic acid (SA) isolated from sisal waste against gastrointestinal nematodes of goats, using the egg hatching and larvae motility assays. In addition, potential cytotoxicity of SA on Vero cell cultures was investigated by means of MTT (3-4,5-dimethylthiazol-2-yl, 2,5diphenyltetrazolium bromide) test. The SA induced a significant inhibition of egg hatching (P<0.05) at all concentrations tested (60 to 250µg mL-1), and the concentrations to inhibit 50% (EC50) and 90% (EC90) values (mean ± standard deviation) were 90.3±2.8 and 130.6±3.5µg mL-1, respectively. The SA has not shown larvicidal activity. The SA was less toxic to the Vero cells, with the mean percentage of cell viability equal to 85±6.2% at the concentration of 130µg mL-1. The results suggested that SA has potential anthelmintic effect; although, more research is needed to confirm its activity in vivo.

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Anthelmintic resistance in gastrointestinal nematodes in goats and evaluation of FAMACHA diagnostic marker in Uganda

Cited by 32 publications

References 28 publications

Evaluation of parasite resistance to commonly used commercial anthelmintics in meat goats on humid subtropical pasture

Evaluation of parasite resistance to commonly used commercial anthelmintics in meat goats on humid subtropical pasture

Media Penyimpanan Telur, Larva dan Cacing Nematode sebagai Media Uji In Vitro

Ovicidal activity of succinic acid isolated from sisal waste (Agave sisalana) against gastrointestinal nematodes of goats

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