Ovicidal activity of succinic acid isolated from sisal waste (Agave sisalana) against gastrointestinal nematodes of goats

Santos, Nathália Silva de Souza; Santos, José Amâncio Macedo; Santos, Francianne Oliveira; Serra, Taiane Menezes; Lima, Hélimar Gonçalves de; Botura, Mariana Borges; Branco, Alexsandro; Batatinha, Maria José Moreira

doi:10.1590/0103-8478cr20170036

Cited by 3 publications

(2 citation statements)

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“…With respect to azelaic acid specifically, identified its possible effect on Caenorhabditis elegans [ 82 ], which was suggested to be associated with the activity of the steroid enzyme 5 alpha-reductase [ 83 ]. Other authors have reported AA against nematodes such as Meloidogyne incognita [ 84 ], Oesophagostomum dentatum [ 85 ], Haemonchus spp., Oesophagostomum spp., and Trichostrongylus [ 86 ]. Moreover, it can be observed a combined effect of fatty acids and tannins on larvae of Toxocora canis [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Anthelmintic Evaluation of Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce: Fingerprint Analysis of Extracts by UHPLC-Orbitrap Mass Spectrometry

et al. 2020

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In the present work, the anthelmintic activity (AA) of ethanolic extracts obtained from Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce was evaluated using the third-stage-larval (L3) exsheathment inhibition test (LEIT) and egg hatch test (EHT) on Haemonchus contortus. Extracts were tested at concentrations of 0.3, 0.6, 1.2, 2.5, 5.0, 10, 20, and 40 mg/mL. The larval exsheathment inhibition (LEI) results showed that G. sepium achieved the highest average inhibition of 91.2%, compared with 44.6% for P. dulce and 41.0% for L. leucocephala at a concentration of 40 mg/mL; the corresponding IC50 values were 22.4, 41.7, and 43.3 mg/mL, respectively. The rates of egg hatching inhibition (EHI) at a concentration of 5 mg/mL were 99.5% for G. sepium, 64.2% for P. dulce, and 54% for L. leucocephala; the corresponding IC50 values were 1.9 mg/mL for G. sepium, 3.9 mg/mL for P. dulce, and 4.3 mg/mL for L. leucocephala. The species extracts studied here were also analyzed by ultra-high performance liquid chromatography and Orbitrap high resolution mass spectrometry (UHPLC-Q/Orbitrap/MS/MS), resulting in the compounds’ identification associated with AA. Glycosylated flavonoids and methoxyphenols were observed in all three species: fatty acids in G. sepium and P. dulce; phenylpropanoids, anthraquinone glycosides, amino acids and glycosylated phenolic acids in G. sepium; and flavonoids in L. leucocephala. Comparatively, G. sepium presented a greater diversity of compounds potentially active against the control of gastrointestinal nematodes, which was associated with the results obtained in the applied tests.

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