Antarcticibacterium flavum gen. nov., sp. nov., isolated from marine sediment

Abstract

A Gram-stain-negative, strictly aerobic, yellow-pigmented, non-gliding, oval to rod-shaped bacterial strain, designated JB01H24, belonging to the family Flavobacteriaceae, was isolated from marine surface sediment collected from the Ross Sea, Antarctica. Strain JB01H24 grew at 4-40 °C (optimum 25-30 °C), pH 7.0-9.0 (optimum 7.5-8.0), and in the presence of 0-8 % NaCl (optimum 3 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JB01H24 formed an independent linkage within the family Flavobacteriaceae and was closely related with the genus Gillisia. Strain JB01H24 exhibited 16S rRNA gene sequence similarities of 95.3-91.5 % and 94.9-94.0 % to the type strains of the genera Gillisia and Salinimicrobium, respectively. The major fatty acids (>5 %) were iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), anteiso-C15 : 0, iso-C15 : 1 G and summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0). The major polar lipids were phosphatidylethanolamine, seven unidentified lipids, two unidentified aminolipids and an unidentified aminophospholipid. Strain JB01H24 contained menaquinone-6 as the only ubiquinone. The DNA G+C content was 42.4 mol%. On the basis of phylogenetic, physiological and chemotaxonomic properties, strain JB01H24 is considered to represent a novel species of a new genus within the family Flavobacteriaceae, for which the name Antarcticibacterium flavum gen. nov., sp. nov. is proposed. The type strain of Antarcticibacterium flavum is JB01H24 (=GDMCC 1.1229=KCTC 52984).

Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, Antarctica

“…In the present study, spread-plate method was used as parallel complement of culture-independent approach and yielded 629 isolates, which would be helpful to perform more metabolic, physiological and ecological tests in future. Our previous studies have phenotypically, phylogenetically and chemotaxonomically characterized five novel type strains out of the 629 isolates (Li et al, 2017a,b, 2018a,b,c).…”

“…Two gram of each sediment stored at 4°C was measured out with a stainless steel spoon and subsequently resuspended in 20 mL sterile seawater. The suspension were serially diluted and spread on Marine Agar 2216 (MA, BD Difco, MD, USA), MC (Li et al, 2017a,b, 2018a,b,c) and LC (1/100 MC) plates. The suspension plating process was performed in parallel to generate two analogous groups of plates, which were incubated at 4°C for 30 days and at 25°C for 7 days, respectively.…”

Pelagihabitans pacificus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a deep-sea seamount

“…Members of the family occur in marine environments [3, 4]. Most novel genera within this family described recently were isolated from marine environments [5–11]. In this study, we report the characterization of a novel species in a novel genus within the family Flavobacteriaceae isolated from a deep-sea seamount.…”

Marinobacter denitrificans sp. nov., isolated from marine sediment of southern Scott Coast, Antarctica

“…Cell motility was examined according to the hanging-drop method [38]. Growth tests under different pH, temperatures and NaCl concentrations were performed as described previously [39]. Other phenotypic characteristics such as enzyme activities, acid production by fermentation and utilization of carbon sources were determined using API ZYM, 20E and 20NE kits (bioMérieux) and Biolog GEN III MicroPlates (Biolog), following the manufacturers’ instructions.…”