Pelagihabitans pacificus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a deep-sea seamount

Abstract

<jats:p>The Gram-stain-negative, orange-pigmented, non-spore-forming, non-motile, strictly aerobic, rod-shaped bacterial strain, designated TP-CH-4<jats:sup>T</jats:sup>, was isolated from a seamount near the Yap Trench in the tropical western Pacific. The optimal growth conditions were determined to be at pH 7–8, 25–30 °C and in the presence of 2 % (w/v) NaCl. The major respiratory quinone was MK-6. The polar lipid profile contained phosphatidylethanolamine, two unidentified aminolipids, two unidentified phospholipids and three unidentified polar lipids. The predominant cellular fatty acids were iso-C<jats:sub>15 : 0</jats:sub> and summed feature 1 (composed of C<jats:sub>13 : 0</jats:sub>3-OH and/or iso-C<jats:sub>15 : 1</jats:sub>H). Phylogenetic analysis of 16S rRNA gene sequences revealed that strain TP-CH-4<jats:sup>T</jats:sup> was a member of the family <jats:italic> <jats:named-content content-type="family"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.8070" xlink:type="simple">Flavobacteriaceae</jats:ext-link> </jats:named-content> </jats:italic> and formed a distinct lineage. Strain TP-CH-4<jats:sup>T</jats:sup> displayed highest sequence similarities to <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.14212" xlink:type="simple">Pseudozobellia thermophila</jats:ext-link> </jats:named-content> </jats:italic> KMM 3531<jats:sup>T</jats:sup> (95.1 %) and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.33010" xlink:type="simple">Flagellimonas flava</jats:ext-link> </jats:named-content> </jats:italic> A11<jats:sup>T</jats:sup> (93.9 %). Genome sequencing revealed the strain TP-CH-4<jats:sup>T</jats:sup> has a genome size of 4.5 Mbp and a G+C content of 44.5 mol%. Collectively, based on phenotypic, chemotaxonomic, phylogenetic and genomic evidence, strain TP-CH-4<jats:sup>T</jats:sup> represents a novel species of a novel genus of the family <jats:italic> <jats:named-content content-type="family"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.8070" xlink:type="simple">Flavobacteriaceae</jats:ext-link> </jats:named-content> </jats:italic>, for which the name <jats:italic>Pelagihabitans pacificus</jats:italic> gen. nov., sp. nov. is proposed. The type strain of <jats:italic>Pelagihabitans pacificus</jats:italic> is TP-CH-4<jats:sup>T</jats:sup> (=CGMCC 1.17120<jats:sup>T</jats:sup>=KCTC 72434<jats:sup>T</jats:sup>).</jats:p>

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“…Genomic DNAs were extracted using a bacterial genomic DNA Mini kit (TaKaRa Bio) according to the manufacturer's protocol. The gene encoding 16S rDNA was amplified, cloned and sequenced following a previously described procedure ( Wang, Liu, & Zhang, 2020 ). The evolutionary history was inferred using the Neighbor-Joining method ( Saitou, 1987 ).…”

Hwangdonia lutea sp. nov., isolated from the Haima cold seep, South China Sea

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Marixanthotalea marina gen. nov., sp. nov., a bacterium in the family Flavobacteriaceae isolated from seawater

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