Antagonistic properties of species-groups of Trichoderma

Dennis, C.; Webster, John

doi:10.1016/s0007-1536(71)80077-3

Cited by 628 publications

(162 citation statements)

References 16 publications

Supporting

Mentioning

148

Contrasting

Unclassified

Order By: Relevance

“…Plates were incubated at 20 ± 2°C. After 7 days of incubation, percent inhibition (PI) of mycelial growth was calculated with the formula proposed by Dennis and Webster (1971). Mycelial plugs of the pathogen from the zone of interaction with antagonists were excised from the dual culture plates and subcultured on fresh PDA medium to assess the viability of antagonized mycelia.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Biocontrol Potentials of Antimicrobial Peptide Producing Bacillus Species: Multifaceted Antagonists for the Management of Stem Rot of Carnation Caused by Sclerotinia sclerotiorum

et al. 2017

View full text Add to dashboard Cite

Bacillus species are widely exploited as biocontrol agents because of their efficiency in impeding various plant pathogens with multifaceted approach. In this study, Bacillus species were isolated from rhizosphere of various plants viz., carnations, cotton, turmeric, and bananas in Tamil Nadu state of India. Their potential to control the mycelial growth of Sclerotinia sclerotiorum was assessed in vitro by dual plate and partition plate techniques. B. amyloliquefaciens strain VB7 was much effective in inhibiting mycelial growth (45% inhibition of over control) and sclerotial production (100%). PCR detection of AMP genes revealed that B. amyloliquefaciens (VB7) had a maximum of 10 diverse antibiotic biosynthesis genes, namely, ituD, ipa14, bacA, bacD, bamC, sfP, spaC, spaS, alba, and albF, that resulted in production of the antibiotics iturin, bacilysin, bacillomycin, surfactin, subtilin, and subtilosin. Further, metabolites from B. amyloliquefaciens strains VB2 and VB7, associated with inhibition of S. sclerotiorum, were identified as phenols and fatty acids by gas chromatography mass spectrometry (GC-MS). Delivery of bacterial suspension of the effective strains of Bacillus spp. as root dip was found promising for the management of stem rot of cultivated carnations. Minimal percent disease incidence (4.6%) and maximum plant growth promotion was observed in the plants treated with B. amyloliquefaciens (VB7).

show abstract

Section: Methodsmentioning

confidence: 99%

“…The experiment was replicated thrice. After 7 days of incubation, the percent inhibition of sclerotial production was calculated as proposed by Dennis and Webster (1971). …”

Section: Methodsmentioning

confidence: 99%

Biocontrol Potentials of Antimicrobial Peptide Producing Bacillus Species: Multifaceted Antagonists for the Management of Stem Rot of Carnation Caused by Sclerotinia sclerotiorum

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Several fungi used in biocontrol have been known for a long time to produce compounds with potent antibiotic activity. Particularly in the genera Chaetomium (4,5,13,14), Gliocladium (6,(15)(16)(17)(18), Penicillium (19), and Trichoderma (20)(21)(22)(23)(24)(25), production of antifungal metabolites has been reported. The importance of these compounds in biocontrol of plant disease has long been questioned.…”

Section: Fungal Antibiotics In Biocontrolmentioning

confidence: 99%

Fungal Antibiosis in Biocontrol of Plant Disease

Pietro

1994

ACS Symposium Series

View full text Add to dashboard Cite

The implication of antibiotics and enzymes of fungal origin in biocontrol has been extensively investigated, but only in a few cases has their role been conclusively elucidated. Production of the antifungal epidithiadiketopiperazine metabolites chaetomin and gliotoxin by the soil-inhabiting biocontrol fungi, Chaetomium globosum and Gliocladium virens, respectively, has been shown to be of importance in biocontrol. Recently, the antifungal properties of purified chitinolytic and glucanolytic enzymes from the biocontrol fungi Trichoderma harzianum and G. virens have been described, and evidence has been provided that these cell wall-degrading enzymes may act synergistically with antibiotics. Recombinant DNA technology, allowing the construction of genetically modified biocontrol agents will be useful for evaluating the role of specific compounds in biocontrol and for creating improved biocontrol organisms. Potential applications of this approach in future biocontrol practice is discussed.

show abstract

“…Different factors involved in the antagonistic properties of Trichoderma have been identified, including antibiotics (4)(5)(6)(7)(8) and hydrolytic enzymes such as 1,3-3-glucanases, proteases, and chitinases (9).…”

mentioning

confidence: 99%

Characterization of ech-42, a Trichoderma harzianum endochitinase gene expressed during mycoparasitism.

Carsolio

Gutiérrez

Jiménez

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

228

156

View full text Add to dashboard Cite

The soil fungus Trichoderma harzianum has been shown to act as a mycoparasite against a range of economically important aerial and soil-borne plant pathogens, being successfully used in the field and greenhouse (1-3). Different factors involved in the antagonistic properties of Trichoderma have been identified, including antibiotics (4-8) and hydrolytic enzymes such as 1,3-3-glucanases, proteases, and chitinases (9).The interaction between Trichoderma and its host is first detectable as chemotropic growth of hyphae of the mycoparasite toward its host (10,11). When the mycoparasite reaches the host, its hyphae often coil around it or are attached to it by forming hook-like structures (12)(13)(14)(15)(16). After these interactions, the mycoparasite penetrates the host mycelium, apparently by partially degrading its cell wall (12,13). Microscopic observations (6,10,13,(17)(18)(19) suggest that Trichoderma spp. produces and excretes mycolytic enzymes, as indicated by localized cell wall lysis at points of interaction. The susceptible host hyphae show rapid vacuolation, collapse, and disintegration (10). Thus, the mycoparasitic process involves (i) chemotropic growth of Trichoderma, (ii) recognition of the host by the mycoparasite and attachment, (iii) excretion of extracellular enzymes, (iv) hyphae penetration, and (v) lysis of the host.Chitin and 1,3-3-glucan are the two major structural components of the cell wall of many plant pathogenic fungi.Therefore, it is expected that the 1,3-3-glucanases, chitinases, and proteases produced extracellularly by Trichoderma (18,(20)(21)(22)(23) play an important role in biocontrol.

show abstract

Antagonistic properties of species-groups of Trichoderma

Cited by 628 publications

References 16 publications

Biocontrol Potentials of Antimicrobial Peptide Producing Bacillus Species: Multifaceted Antagonists for the Management of Stem Rot of Carnation Caused by Sclerotinia sclerotiorum

Biocontrol Potentials of Antimicrobial Peptide Producing Bacillus Species: Multifaceted Antagonists for the Management of Stem Rot of Carnation Caused by Sclerotinia sclerotiorum

Fungal Antibiosis in Biocontrol of Plant Disease

Characterization of ech-42, a Trichoderma harzianum endochitinase gene expressed during mycoparasitism.

Contact Info

Product

Resources

About