Antagonistic effects of indoloquinazoline alkaloids on antimycobacterial activity of evocarpine

Hochfellner, Christina; Evangelopoulos, Dimitrios; Zloh, Mire; Wube, Abraham Abebe; Guzman, Juan; McHugh, Timothy D.; Kunert, Olaf; Bhakta, Sanjib; Bučar, Franz

doi:10.1111/jam.12753

Cited by 27 publications

(11 citation statements)

References 29 publications

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“…Metabolic activity of C. jejuni with possible degradation of extract constituents might also play a role. Furthermore, antagonism between compounds present in the same plant extract has already been shown in the case of antimycobacterial effect of the quinolinone alkaloid 9 (Hochfellner et al, 2015). Therefore, the potential interactions between compounds within an extract should not be overlooked.…”

Section: Discussionmentioning

confidence: 99%

Attenuation of Adhesion, Biofilm Formation and Quorum Sensing ofCampylobacter jejunibyEuodia ruticarpa

et al. 2016

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Thermophilic campylobacters are a major cause of bacterial food-borne diarrhoeal disease. Adherence and biofilm formation are key elements of Campylobacter jejuni persistence in unfavourable environmental conditions. The phytochemical analysis of Euodia ruticarpa fruit ethanol solution extract (EREE) indicated that the major compounds were evodiamine (1), rutaecarpine (2) and evocarpine (9). E. ruticarpa fruit ethanol solution extract, compounds 1 and 2 as well as a mixture of quinolinone alkaloids with 41.7% of 9 were tested for antibacterial, antibiofilm and antiquorum sensing activities against C. jejuni. Minimal inhibitory concentrations varied from 64 to 1024 µg/mL. A mutant strain that lacks the functional gene coding for the CmeB efflux pump protein was the most susceptible. Interestingly, in addition to the wild-type (NCTC 11168) and cmeB mutant, also a mutant that lacks autoinducer-2 production (luxS) was able to adhere (1 h) and to produce a biofilm (24, 48 and 72 h). The subinhibitory concentrations of all preparations at least partly inhibited C. jejuni adhesion and biofilm formation with the most visible effect of the quinolinone alkaloid fraction. Using a Vibrio harveyi luminescence assay, the inhibition of autoinducer-2 production was observed in the wild-type and cmeB mutant after 48 h with the most visible effect of EREE and its fraction Q. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

Attenuation of Adhesion, Biofilm Formation and Quorum Sensing ofCampylobacter jejunibyEuodia ruticarpa

et al. 2016

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“…Even though our data did not support any synergy between the oleoresins or extracts and isoniazid, we investigated the combination of these compounds in an attempt to improve the antimycobacterial activity. Hochfellner et al [ 51 ] evaluated the interaction between quinolone and indoloquinazoline alkaloids with rifampicin or isoniazid against Mycobacterium smegmatis (ATCC 14468). The authors did not find satisfactory interaction between the evaluated molecules and rifampicin or isoniazid.…”

Section: Resultsmentioning

confidence: 99%

Investigation of Copaifera Genus as a New Source of Antimycobaterial Agents

et al. 2020

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Aim: This paper reports on the antimycobacterial activity of the oleoresins and extracts obtained from Copaifera spp. Materials & methods: The minimum inhibitory concentration (MIC) and fractional inhibitory concentration index techniques helped to evaluate the effect of these oleoresins and extracts against six strains of mycobacteria that cause tuberculosis. Results & conclusion: Among the assayed oleoresins and plant extracts, the Copaifera langsdorffii, Copaifera duckei, Copaifera reticulata and Copaifera trapezifolia oleoresins provided the lowest MIC values against some of the tested strains. The combination of Copaifera spp. samples with isoniazid did not evidence any synergistic action. Some Copaifera spp. oleoresins may represent a future source for the discovery of new antimycobacterial drugs due to their low MIC values.

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“…Interestingly, in a previous study of the antimycobacterial activities of a hexane extract of E. rutaecarpa, evocarpine, and the indoloquinazolines evodiamine and rutaecarpine, antagonistic actions were seen for evodiamine and rutaecarpine on the effects of evocarpine, which were most likely due to their formation Fig. 4 Structures of compounds that show anti-adhesive or anti-biofilm activities against C. jejuni exposed to abiotic surfaces of a complex in solution (Hochfellner et al 2015). Hence, such interactions cannot be excluded when plant extracts or multicomponent fractions are studied.…”

Section: Anti-adhesion and Anti-biofilm Compounds Of Natural Originmentioning

confidence: 99%

Anti-adhesion activity of phytochemicals to prevent Campylobacter jejuni biofilm formation on abiotic surfaces

et al. 2020

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Biofilms provide a protective environment for pathogens such as Campylobacter jejuni, the most prevalent foodborne pathogen, and biofilm formation can enhance bacterial survival in hostile environments. Adhesion of bacteria to the different materials of industrial surfaces is the first step in biofilm formation. Modulation of bacterial adhesion and biofilm formation thus represent important targets in alternative control strategies for reduction of pathogens in food-processing environments. With the high prevalence of C. jejuni and the lack of effective control measures, new control strategies are needed to block adhesion and biofilm formation on food contact surfaces in the food industry, with a focus here on natural antimicrobial phytochemicals. Plants remain a poorly recognized yet vast source of such antimicrobials. Valuable phytochemicals can be obtained directly from plant materials but also from agro-food by-products and waste materials. These materials represent a source of important plant bioactive phytochemicals that are effective for prevention of bacterial adhesion. In this review, we will focus on the anti-adhesion activities of phytochemicals targeted against C. jejuni, on the appropriate methodologies to determine anti-adhesion effects of phytochemicals, on the mechanisms of C. jejuni adhesion, and thus possible targets for reduction and control of this foodborne pathogen in food processing environments.

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Antagonistic effects of indoloquinazoline alkaloids on antimycobacterial activity of evocarpine

Abstract: This study gives a striking example of antagonism between compounds present in the same plant extract which should be considered in natural product based screening projects.

Cited by 27 publications

References 29 publications

Attenuation of Adhesion, Biofilm Formation and Quorum Sensing ofCampylobacter jejunibyEuodia ruticarpa

Attenuation of Adhesion, Biofilm Formation and Quorum Sensing ofCampylobacter jejunibyEuodia ruticarpa

Investigation of Copaifera Genus as a New Source of Antimycobaterial Agents

Anti-adhesion activity of phytochemicals to prevent Campylobacter jejuni biofilm formation on abiotic surfaces

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