Attenuation of Adhesion, Biofilm Formation and Quorum Sensing ofCampylobacter jejunibyEuodia ruticarpa

Abstract

Thermophilic campylobacters are a major cause of bacterial food-borne diarrhoeal disease. Adherence and biofilm formation are key elements of Campylobacter jejuni persistence in unfavourable environmental conditions. The phytochemical analysis of Euodia ruticarpa fruit ethanol solution extract (EREE) indicated that the major compounds were evodiamine (1), rutaecarpine (2) and evocarpine (9). E. ruticarpa fruit ethanol solution extract, compounds 1 and 2 as well as a mixture of quinolinone alkaloids with 41.7% of 9 were tested for antibacterial, antibiofilm and antiquorum sensing activities against C. jejuni. Minimal inhibitory concentrations varied from 64 to 1024 µg/mL. A mutant strain that lacks the functional gene coding for the CmeB efflux pump protein was the most susceptible. Interestingly, in addition to the wild-type (NCTC 11168) and cmeB mutant, also a mutant that lacks autoinducer-2 production (luxS) was able to adhere (1 h) and to produce a biofilm (24, 48 and 72 h). The subinhibitory concentrations of all preparations at least partly inhibited C. jejuni adhesion and biofilm formation with the most visible effect of the quinolinone alkaloid fraction. Using a Vibrio harveyi luminescence assay, the inhibition of autoinducer-2 production was observed in the wild-type and cmeB mutant after 48 h with the most visible effect of EREE and its fraction Q. Copyright © 2016 John Wiley & Sons, Ltd.

Application of β-Resorcylic Acid as Potential Antimicrobial Feed Additive to Reduce Campylobacter Colonization in Broiler Chickens

“…Since Campylobacter adhesion to epithelial cells is an important step for colonization (Jin et al, 2001; Hermans et al, 2011a), a reduction in this virulence attribute could potentially reduce colonization in birds. Bezek et al (2016) had similar findings with extracts from Euodia ruticarpa . In addition, these researchers observed that other virulence attributes, including biofilm formation and quorum sensing were also affected by the phytochemical.…”

Modulation of Campylobacter jejuni Motility, Adhesion to Polystyrene Surfaces, and Invasion of INT407 Cells by Quorum-Sensing Inhibition

“…Furthermore, these citrus extracts reduced C. jejuni invasion of and adhesion to HeLa cells and the expression of the virulence genes cadF and ciaB [26]. On the other hand, Bezek et al [27] showed good C. jejuni QS reduction with Euodia ruticarpa but no connection to C. jejuni biofilm formation. This indicates high sensitivity of the C. jejuni LuxS system, and thus QS signaling, to outside stressors, such as treatments with natural extracts.…”

“…The AI-2 bioassays were performed as previously described [27,28] with some modifications. These QS inhibition bioassays were carried out using the biosensor reporter strain V. harveyi BB170 [27], which was grown for 16 h at 30 • C and 150 rpm mixing to approximately 10 7 CFU/mL and used at the final concentration of 5 × 10 3 CFU/mL in AB medium. The C. jejuni CFSs were added to the suspensions of the reporter strain to a final concentration of 10% (v/v) (i.e., 20 µL CFS added to 180 µL reporter strain suspension).…”

Antiadhesion activity of juniper (Juniperus communis L.) preparations against Campylobacter jejuni evaluated with PCR‐based methods

“…The surfaces of equipment used for food handling, storage, and processing represent major sources of Campylobacter contamination (Nguyen et al, ; Simões et al, ). As is known, C. jejuni forms monospecies biofilms on stainless steel, glass (e.g., fibre, beads, coverslips), nitrocellulose membranes, and various plastic surfaces (Bezek et al, ; Teh et al, ). Campylobacter attached to food contact surfaces can form biofilms and survive not just the food processing conditions (as has been seen for the dairy industry and during meat processing) but also the addition of antimicrobials and other disinfection treatments (Joshua et al, ).…”