Another Example of Anti‐HL‐A8 Reacting by Platelet Complement Fixation Microtechnique

Mouzon, A.; Ohayon, E; J, Pris; Julien, F.

doi:10.1111/j.1423-0410.1976.tb02860.x

Vox Sanguinis

1976

DOI: 10.1111/j.1423-0410.1976.tb02860.x

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Another Example of Anti‐HL‐A8 Reacting by Platelet Complement Fixation Microtechnique

A. Mouzon

E Ohayon

Pris J

et al.

Abstract: We report the study of a serum from a polytransfused patient, that contains an anti-HL-A8 antibody reacting by the platelet complement fixation microtechnique. The specificity is confirmed by a study on a panel of 112 different platelets and by experiments of absorption-elution on platelets, lymphocytes and granulocytes.

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Technical and theoretical considerations in the HLA typing of amniotic fluid cells for prenatal diagnosis and paternity testing

Pollack

Braun

et al. 1981

Prenatal Diagnosis

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HLA typing of amniotic fluid cells has been used for the prenatal diagnosis of the HLA linked diseases congenital adrenal hyperplasia (21-OH-deficiency (21-OH-def) type) and complement C4 deficiency and it has also been used for the prenatal determination of paternity. There are, however, technical difficulties in this test associated with the weak expression of some B locus antigens on amniotic fluid cells, and theoretical difficulties related to associations between particular HLA antigens and the 21-OH-def allele. Since certain HLA-B locus antigens are found in significantly increased frequencies among patients with 21-OH-def, there is a relatively high incidence of HLA-B homozygosity among the patients and over 40 per cent of the parents of these patients share one or more HLA-B locus antigens. Results of some prenatal HLA typing tests may thus be difficult to interpret, and supplementary tests should be used whenever possible. HLA typing of amniotic cells is, however, the only available procedure for prenatal diagnosis of C4 deficiency and it is the best available procedure for prenatal determination of paternity. A modification of our original procedure allows HLA typing to be performed with increased numbers of HLA typing sera, and sera with optimum reactivity for amniotic fluid cells have now been selected for the definition of most of the more commonly expressed HLA antigens. Although amniotic fluid cells do not express DR Antigens, amniotic fluid cells can be typed for the HLA-linked marker glyoxalase I (GLO) and this may be the informative for prenatal diagnosis in some cases.

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Technical and theoretical considerations in the HLA typing of amniotic fluid cells for prenatal diagnosis and paternity testing

Pollack

Braun

et al. 1981

Prenatal Diagnosis

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Retention of HLA Antigens on Previously Frozen Human Platelets

et al. 1980

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Recent technical achievements allow satisfactory cryopreservation of human platelets that, after thawing and washing, maintain functional viability for therapeutic use. We have now demonstrated that such previously frozen platelets retain full HLA antigenic activity in spite of the combination of freezing, thawing, and washing procedures and the presence of the surface-active cryopreservative DMSO. Platelets stored at 4 C without DMSO and previously frozen platelets from the same donors were tested in parallel for quantitative expression of HLA antigens. In all cases, the previously frozen platelets were quantitatively equal or superior to the platelets stored at 4 C with regard to their capacity to specifically reduce the HLA antibody activities of selected typing sera against a panel of antigen-positive lymphocytes.

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Another Example of Anti‐HL‐A8 Reacting by Platelet Complement Fixation Microtechnique

Cited by 2 publications

References 0 publications

Technical and theoretical considerations in the HLA typing of amniotic fluid cells for prenatal diagnosis and paternity testing

Technical and theoretical considerations in the HLA typing of amniotic fluid cells for prenatal diagnosis and paternity testing

Retention of HLA Antigens on Previously Frozen Human Platelets

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