Retention of HLA Antigens on Previously Frozen Human Platelets

Pollack, Marilyn S.; Zaroulis, Charles G.; Klainbard, J.; Leiderman, Ira Z.; Dupont, Bo

doi:10.1046/j.1537-2995.1980.20480260281.x

Cited by 11 publications

(5 citation statements)

References 13 publications

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“…Schiffer & Young (1983) have tried different methods of platelet preservations and found that liquid suspension in normal saline may still be suitable for platelet crossmatching after one year of storage. Nevertheless, one study negates reduction of HLA antigens after freezing, thawing and washing (Pollack et al 1980). Our study found that virtually all the frozen platelets could retain their antigenicity within one month.…”

Section: Discussionmentioning

confidence: 51%

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Antigen expression of frozen platelets

Chow

Tzeng

et al. 2008

Clinical &Amp; Laboratory Haematology

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Summary Platelet antigens of platelet samples from 36 donors, frozen for different intervals, were evaluated by the platelet suspension immunofluorescence test (PSIFT). A, B, PLA1(HPA‐la) and various HLA antigens were tested by their corresponding antisera. The antigen could be detected in almost all the samples after one month of freezing. After 3 and 6 months, the platelet antigens could only be detected in 29.2% and 3.7% of the samples, respectively. There was no difference in decay of antigen expression among A, B, PLA1 and HLA antigens. When compared with the freshly prepared platelets, frozen platelets presented stronger antigen expression after 2 to 4 weeks of storage. This may suggest that the frozen platelets could be used for platelet crossmatching procedures without loss of their antigenicity within one month.

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Section: Discussionmentioning

confidence: 51%

“…It has been noted that the expression of HLA antigen on platelets is variable (Schiffer, O'Connell & Lee 1989, Aster et al 1977). Nevertheless, one study negates reduction of HLA antigens after freezing, thawing and washing (Pollack et al 1980). We also observed that the HLA antigens could be preserved and detectable for one month.…”

Section: Discussionmentioning

confidence: 58%

Antigen expression of frozen platelets

Chow

Tzeng

et al. 2008

Clinical &Amp; Laboratory Haematology

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“…However, to function successfully, stored platelet samples must be available on the HLA-typed donors to be used for crossmatching. There are a variety of techniques available to store platelets, but, with many, antigen reactivity is lost over time (61)(62)(63)(64). In addition, to prepare and store platelet samples on a large number of donors is a time-consuming and costly endeavor.…”

Section: Platelet Crossmatch Testingmentioning

confidence: 99%

Platelet Transfusions a Constantly Evolving Therapy

Slichter¹

1991

Thromb Haemost

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“…Although HLA-A,B,C antigens on platelets have a varia ble expression in relation to lymphocytes [Colombani et al, 1971;Liebert and Aster, 1977;Mueller-Eckhardt et al, 1978, 1980aSzatkowski and Aster, 1980], they lend themselves to HLA typing and antibody screening [Colombani et al, 1971 ;Svejgaard and Kissmeyer-Nielsen, 1970;Heinrich and Mueller-Eckhardt, 1971 ;Degos et al, 1975], Due to their lack of D-related HLA antigens, they are also widely used to absorb HLA-A,B,C antibod ies from sera containing HLA class II antibodies. For these purposes, platelets can be stored for many months at 4°C, or in the frozen state without loss of HLA-A,B,C antigenicity Pollack et al, 1980]. Furthermore, HLA-incompatible platelets have been shown to have a shortened survival time when transfused into HLA-immunized patients.…”

Section: Adsorption In Vivomentioning

confidence: 99%

HLA Antigens on Platelet Membranes

Mueller‐Eckhardt¹,

Santoso²,

Kiefel³

et al. 1986

Vox Sanguinis

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In order to determine whether HLA-A,B antigens of platelets are integral membrane constituents or rather represent adsorbed plasma proteins, their presence in plasma and their adsorbability onto platelet membranes was studied by in vitro and in vivo experiments. The amount of HLA antigens was quantitated by inhibition of lymphocytotoxicity (LCT) and by enzyme-linked immunosorbent assay (ELISA) using operationally monospecific polyclonal HLA antibodies or murine HLA-specific monoclonal antibodies, respectively. We found that in 11 out of 13 HLA-A2 and in 9 out of 10 HLA-B13 experiments, platelets from antigen-negative donors pretreated with plasma from the same number of antigen-positive donors inhibited LCT to the same extent as platelets from antigen-positive donors. Nevertheless, the in vitro adsorbed HLA antigens onto antigen-negative platelets were, unlike those on antigen-positive platelets or in plasma, not reactive with monoclonal antibodies as quantitated by ELISA. Similarly, infusion of HLA-A2-negative platelets from single donors into 3 HLA-A2-positive, thrombocytopenic patients with bone marrow failure led to a good platelet increment, but did not convert the HLA type of donor platelets, neither at 2 h nor at 18 h posttransfusion. On the basis of these results, we conclude that soluble HLA antigens can be taken up by human platelets from plasma in small amounts. However, the major portion of HLA antigens appears to be integral membrane constituents.

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Retention of HLA Antigens on Previously Frozen Human Platelets

Cited by 11 publications

References 13 publications

Antigen expression of frozen platelets

Antigen expression of frozen platelets

Platelet Transfusions a Constantly Evolving Therapy

HLA Antigens on Platelet Membranes

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