Annual profile of mitogen-activated protein kinase (extracellular signalregulated kinase 1 and 2) in the frog(Rana esculenta) testis

Chieffi, Paolo; D’Amato, Luca Colucci; Tramontano, Donatella

doi:10.1007/bf02904519

Cited by 8 publications

(9 citation statements)

References 29 publications

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“…Otherwise, in L. pipiens estrogens retard the formation of secondary spermatogonia and spermatocytes and accelerate the final stages of spermatogenesis (Tsai et al, 2003). Furthermore, E 2 is associated with high mitogenic activity of spermatogonia in P. esculentus (Fasano et al, 1989;Rastogi et al, 1985;Varriale et al, 1986) via Fos and mitogen-activated kinase (Chieffi et al, 1995(Chieffi et al, , 2000Cobellis et al, 1997Cobellis et al, , 1999Cobellis et al, , 2002Minucci et al, 1997). Our results in R. arenarum indicate that in spite of the season, estradiol has no effect on spermatogonial proliferation.…”

Section: Discussionmentioning

confidence: 47%

“…Besides, in P. esculentus there is a strong mitogenic activity of spermatogonia in the period of the year characterized by high concentrations of estradiol (Fasano et al, 1989;Rastogi et al, 1985;Varriale et al, 1986). In this species, estradiol promotes spermatogonial proliferation through the activity of Fos protein and the activation of mitogen-activated protein kinase in spermatogonia (Chieffi et al, 1995(Chieffi et al, , 2000Cobellis et al, 1999;Minucci et al, 1997) and proliferation is inhibited by melatonin (d Istria et al, 2003). Moreover, estradiol regulates the expression of Fra-1, which is located in peritubular myoid cells, efferent ducts and blood vessels and may be involved in sperm transport.…”

Section: Introductionmentioning

confidence: 98%

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Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Scaia

Volonteri

Czuchlej

et al. 2015

General and Comparative Endocrinology

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Section: Discussionmentioning

confidence: 47%

Section: Introductionmentioning

confidence: 98%

Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Scaia

Volonteri

Czuchlej

et al. 2015

General and Comparative Endocrinology

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“…CNR1 and FAAH levels were plotted as quantitative densitometry analysis of signals corrected on the basis of invariable MAPK1 content [48]. Values were expressed as optical density (OD) units.…”

Section: Data Presentation and Statisticsmentioning

confidence: 99%

Endocannabinoid System in Frog and Rodent Testis: Type-1 Cannabinoid Receptor and Fatty Acid Amide Hydrolase Activity in Male Germ Cells1

Cobellis¹,

Cacciola²,

Scarpa³

et al. 2006

Biology of Reproduction

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N-arachidonoylethanolamide (anandamide [AEA]) is the main endocannabinoid described to date in the testis. It exerts its effects through the activation of G-protein coupled cannabinoid receptors (CNR). However, the activity of AEA in controlling male reproduction is still poorly known. Here we provide direct evidence on the presence of the "endocannabinoid system," constituted by type-1 cannabinoid receptor (CNR1) and fatty acid amide hydrolase (FAAH), in the frog Rana esculenta testis demonstrating its expression in tubular compartment. In fact, during the annual reproductive cycle, both proteins increase in September, when the appearance of spermatids (SPT) occurs. Immunocytochemistry confirms their localization in germ cells and, in particular, in elongated SPT. Signals are still present in spermatozoa (SPZ), as demonstrated by Western blot analysis. Furthermore, the activation of CNR1 reduces sperm motility. Comparative research, carried out using mouse and rat SPZ, definitely indicates that the endocannabinoid system operates in SPZ of phylogenetically distant species. A conserved physiological role of endocannabinoid system in controlling the inhibition of sperm motility is suggested.

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“…ERKs are expressed ubiquitously and closely related to the yeast protein kinases involved in pheromone induced mating (Nielsen et al, 1993). For example, in the frog R. esculenta and in the lizard Podarcis s. sicula testis different levels of ERK1/2 activity are present during the annual reproductive cycle (Chieffi et al, 2001(Chieffi et al, , 2002; in addition, it has been shown that 17-β estradiol induces ERKs phoshorylation in frog (R. esculenta) (Chieffi et al, 2000b) and lizard (P. s. sicula) testis (Chieffi et al, 2002), and JNK1 has different activity in frog (R. esculenta) (Chieffi, 2003) and lizard (P. s. sicula) testis during the annual reproductive cycle (Chieffi et al, 1999). Recent studies suggest a role for ERK1 in the regulation of spermatogonial proliferation.…”

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confidence: 99%

Environmental influence on testicular MAP kinase (ERK1) activity in the frogRana esculenta

Chieffi

Minucci

2004

Journal of Experimental Biology

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SUMMARY Recent studies suggest a role for ERK1 in the regulation of spermatogonial proliferation. In this report the frog Rana esculenta, a seasonal breeder, was used as a model to study the possible effect on ERK1 of photoperiod and temperature. Adult male R. esculenta were subjected to several combinations of light and temperature at different times of the year to elucidate the regulation of ERK1 testicular activity in the spermatogonial proliferation by these environmental factors. Western blot analysis shows that under controlled experimental conditions an increase of temperature and photoperiod in November, characterized by a decrease in primary spermatogonial mitosis, induces ERK1 activity and spermatogonial proliferation, as confirmed using the proliferating cellular nuclear antigen (PCNA) as an early molecular marker. In contrast, a decrease in temperature and photoperiod in March, with an increase of primary spermatogonial mitosis, impairs ERK1 activity and spermatogonial proliferation. In conclusion, our data clearly show for the first time in a non-mammalian vertebrate that the temperature and the photoperiod exert a role in the spermatogonial proliferation via ERK1 activity.

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Annual profile of mitogen-activated protein kinase (extracellular signalregulated kinase 1 and 2) in the frog(Rana esculenta) testis

Cited by 8 publications

References 29 publications

Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Endocannabinoid System in Frog and Rodent Testis: Type-1 Cannabinoid Receptor and Fatty Acid Amide Hydrolase Activity in Male Germ Cells1

Environmental influence on testicular MAP kinase (ERK1) activity in the frogRana esculenta

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