Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Scaia, María Florencia; Volonteri, María Clara; Czuchlej, Silvia Cristina; Ceballos, Nora R.

doi:10.1016/j.ygcen.2014.12.016

Cited by 11 publications

(12 citation statements)

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“…In adult rat, estradiol seems to promote apoptosis of germ cells except spermatogonia A 2 , spermatogonia B and metaphasic spermatocyte I and the most vulnerable to apoptosis were pachytene spermatocyte I [11,42], spermatocyte II and round and elongated spermatids [42] which lead to a drastic decline in the number of spermatids and spermatozoa [10]. Similar observations were made in amphibia, Rhinella arenarum, where estradiol enhanced spermatocytes' apoptosis during the reproductive season [43]. The abundance of spermatogonial mitosis after the 17bE treatment in P. obesus may reflect its stimulatory effect on proliferation.…”

Section: Discussionsupporting

confidence: 63%

17β-estradiol effect on testicular β-endorphin expression in Psammomys obesus

Madani¹,

Moudilou²,

Exbrayat³

et al. 2015

Folia Histochem Cytobiol.

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Introduction. Testicular function in the sand rodent Psammomys obesus is subjected to seasonal alternations with a trigger of spermatogenesis in winter and a total quiescence which extends from late spring to summer. The aim of this study was to investigate the distribution of b-endorphin in the testis at the period of winter sexual activity and at its summer regression, and assess the effect of 17b-estradiol treatment on testicular morphology and b-endorphin expression. Material and methods. The adult males were grouped into 4 groups (rest group, sexually active group, rest treated with 17b-estradiol group and controls at sexual rest injected with olive oil, n = 5 in each group). Using anti-serum against b-endorphin, we studied its testicular expression by Western blot and cellular location by immunohistochemical (IHC) method, respectively. Results. We detected by Western blot a peptide of 3.5 kDa molecular weight corresponding to b-endorphin only in sexually resting and control males. The 17b-estradiol treatment induced a clear reduction in the b-endorphin band expression compared with the latter. These results were confirmed by the IHC analysis since b-endorphin was only observed in the testis at sexual rest and in controls, in majority of seminiferous tubules at the level of germ cells. The intensity of IHC labeling was significantly different between spermatogonia and spermatocytes I or round spermatids which revealed the strongest labeling. The intense immunoreactivity was also located in Leydig cells and highly significantly varied compared to the germ cells. The 17b-estradiol treatment decreased significantly the b-endorphin signal in germ cells but not in Leydig cells. Conclusion. The 17b-estradiol treatment induces a repressive effect on seasonal testicular endorphinergic system in P. obesus and this action targets exclusively the germ cells.

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Section: Discussionsupporting

confidence: 63%

17β-estradiol effect on testicular β-endorphin expression in Psammomys obesus

Madani¹,

Moudilou²,

Exbrayat³

et al. 2015

Folia Histochem Cytobiol.

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“…However, in other anuran species, E 2 does not seem to be involved in the control of germ cell proliferation. In R. arenarum , in vitro treatment with E 2 did not stimulate spermatogonial proliferation (Scaia et al, ), while in vivo exposure of L. pipiens adults to E 2 delayed the formation of Type II spermatogonia (Tsai et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Testicular serial sections were analyzed for DNA fragmentation using an ApopTag Fluorescein In Situ Apoptosis Detection Kit (Millipore), as described in Scaia et al (). Briefly, sections were treated with proteinase K (20 μg/μl, Millipore) for 15 min and incubated for 1 h with Tdt‐enzyme at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Cellular proliferation was determined in freshly isolated testicular fragments and in BOs by incubation in 4 ml of sterile L15‐medium containing 1 mmol l −1 of 5‐bromo‐2‐deoxyuridine (BrdU). After 1 h at 37°C, organs were processed for immunohistochemistry as described above, and immunohistochemical staining was performed as described by Scaia et al (). S ections of testicular fragments and of BOs collected during the year were incubated overnight at 4°C with a primary monoclonal anti‐BrdU antibody (1:100; GE Healthcare, UK) and then were incubated with a secondary rabbit anti‐mouse polyclonal antibody conjugated with biotin for 1 h at room temperature (1:500; Santa Cruz Biotechnology).…”

Section: Methodsmentioning

confidence: 99%

“…However, there is little evidence regarding the role of estrogens in regulating testicular apoptosis in amphibians. It has recently been demonstrated that in vitro treatment with E 2 induces apoptosis of spermatogenic cysts during the reproductive season in R. arenarum (Scaia et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Apoptosis, proliferation and presence of estradiol receptors in the testes and Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura)

Scaia

Czuchlej

Cervino

et al. 2015

Journal of Morphology

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The dynamic equilibrium between spermatogonial proliferation and testicular apoptosis determines the progression of spermatogenesis in amphibians. Estrogens and their receptors play a central role in regulating spermatogenesis in vertebrates, and in some species of anurans, estradiol (E2 ) is involved in the regulation of spermatogonial proliferation and apoptosis of germ cells. Bidder's organ (BO) is a structure characteristic of Bufonidae that has historically been compared to an undeveloped ovary. In adult Rhinella arenarum males, BO is one of the main sources of plasma E2 . The aim of this study was 1) to describe the seasonal variations in testicular apoptosis, spermatogonial proliferation, and cellular proliferation in BO; and 2) to analyze the presence and localization of estrogen receptor β (ERβ) in the testes and BO of R. arenarum. Testicular fragments and BOs from animals collected during the year were labeled with 5-bromo-2'-deoxyuridine (BrdU) and BrdU incorporation was determined using immunohistochemistry. Apoptosis in testicular sections was detected using the TUNEL method, and ERβ localization was assessed using immunohistochemistry in testes and BOs. The results indicate that spermatogonial proliferation is highest during the reproductive season and that cysts of spermatocytes and spermatids undergo apoptosis during the postreproductive season. Furthermore, the proliferation of follicular cells is highest during the reproductive and postreproductive seasons. ERβ was primarily detected by immunolocalization in Sertoli cells, follicular cells, and oocytes. Taken together, these results suggest that cysts that do not form spermatozoa are removed from testes by apoptosis and that estrogens regulate both spermatogenesis and oogenesis in adult males of R. arenarum.

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Sex Determination and Differentiation in Frogs

Ito

2018

Diversity and Commonality in Animals

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Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Cited by 11 publications

References 63 publications

17β-estradiol effect on testicular β-endorphin expression in Psammomys obesus

17β-estradiol effect on testicular β-endorphin expression in Psammomys obesus

Apoptosis, proliferation and presence of estradiol receptors in the testes and Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura)

Sex Determination and Differentiation in Frogs

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