Annexin I Is an Endogenous Ligand that Mediates Apoptotic Cell Engulfment

Arur, Swathi; Uche, Uche E.; Karim, Rezaul; Fong, Michael C.; Scranton, Victoria; Cowan, Ann E.; Mohler, William A.; Han, David K.

doi:10.1016/s1534-5807(03)00090-x

Cited by 382 publications

(301 citation statements)

References 48 publications

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“…Finally, the membrane expression of ANXA1 after 24 h TRAIL (Figure 1c) confirms previous results in Jurkat cells 5 and suggests that the exposure of ANXA1 on the surface of apoptotic cells may be a widespread mechanism for the recognition and removal of apoptotic cells not restricted to a single cell type. Further work is necessary to clarify the role of ANXA1 as 'eat me' signal in FRO cells.…”

supporting

confidence: 89%

“…Importantly, silencing ANXA1 protein by small interference RNA (siRNA) resulted in defective tethering and engulfment of apoptotic cells. 5 Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the tumor necrosis factor (TNF) family of proteins including FasL, and TNF-a. TRAIL effectively kills most thyroid cancer cell lines tested, including those originating from anaplastic carcinomas.…”

mentioning

confidence: 99%

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Induction of annexin-1 during TRAIL-induced apoptosis in thyroid carcinoma cells

et al. 2005

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We investigated the expression of annexin-1 (ANXA1) in thyroid carcinoma cell lines and in thyroid cancers with a different degree of differentiation. The highest level of ANXA1 expression examined by Western blotting was detected in the papillary carcinoma cells (NPA) and in the follicular cells (WRO). On the other hand, the most undifferentiated thyroid carcinoma cells (ARO and FRO) presented the lowest level of ANXA1 expression. In surgical tissue specimens from 32 patients with thyroid cancers, we found high immunoreactivity for ANXA1 in papillary (PTC) and follicular (FTC) thyroid cancers while in undifferentiated thyroid cancers (UTC) the expression of the protein was barely detectable. Control thyroid tissue resulted positive for ANXA1. In summary, 70% of UTC examined weakly expressed ANXA1, whereas 65% of PTC or FTC specimens tested showed high expression of the protein. Thus ANXA1 expression may correlate with the tumorigenesis suggesting that the protein may represent an effective differentiation marker in thyroid cancer

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confidence: 89%

mentioning

confidence: 99%

Induction of annexin-1 during TRAIL-induced apoptosis in thyroid carcinoma cells

et al. 2005

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“…Recently, it has been suggested that calreticulin may function as a second general recognition ligand, via binding and activating LRP or CD91 on engulfing cells. 14 Arur et al 37 have suggested that annexin I may function in the facilitation of or enhancement of the recognition of PS by the receptor. Whether calreticulin or annexin I is related to stabilin-2-mediated phagocytosis is currently unclear, and will require further study.…”

Section: Discussionmentioning

confidence: 99%

Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor

et al. 2007

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Rapid phagocytic clearance of apoptotic cells is crucial for the prevention of both inflammation and autoimmune responses. Phosphatidylserine (PS) at the external surface of the plasma membrane has been proposed to function as a general 'eat me' signal for apoptotic cells. Although several soluble bridging molecules have been suggested for the recognition of PS, the PS-specific membrane receptor that binds directly to the exposed PS and provides a tickling signal has yet to be definitively identified. In this study, we provide evidence that stabilin-2 is a novel PS receptor, which performs a key function in the rapid clearance of cell corpses. It recognizes PS on aged red blood cells and apoptotic cells, and mediates their engulfment. The downregulation of stabilin-2 expression in macrophages significantly inhibits phagocytosis, and anti-stabilin-2 monoclonal antibody provokes the release of the anti-inflammatory cytokine, transforming growth factor-b. Furthermore, the results of timelapse video analyses indicate that stabilin-2 performs a crucial function in the rapid clearance of aged and apoptotic cells. These data indicate that stabilin-2 is the first of the membrane PS receptors to provide tethering and tickling signals, and may also be involved in the resolution of inflammation and the prevention of autoimmunity.

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“…Further analysis of membrane components in CN and CS conditions may provide more clues about the molecular mechanism of larval arrest upon CS. Furthermore, NEX-1 functions as a ligand that mediates efficient clearance of apoptotic cells by colocalizing with phosphatidylserine (Arur et al, 2003). Therefore, up-regulation of NEX-1 is likely to promote engulfment of cell corpses generated by developmental defects.…”

Section: Discussionmentioning

confidence: 99%

Cholesterol-Responsive Metabolic Proteins Are Required for Larval Development in Caenorhabditis elegans

Kawasaki

Jeong

Yun

et al. 2013

Molecules and Cells

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Caenorhabditis elegans, a cholesterol auxotroph, showed defects in larval development upon cholesterol starvation (CS) in a previous study. To identify cholesterol-responsive proteins likely responsible for the larval arrest upon CS, a comparative proteomic analysis was performed between C. elegans grown in normal medium supplemented with cholesterol (CN) and those grown in medium not supplemented with cholesterol (cholesterol starvation, CS). Our analysis revealed significant change (more than 2.2-fold, p < 0.05) in nine proteins upon CS. Six proteins were down-regulated [CE01270 (EEF-1A.1), CE08852 (SAMS-1), CE11068 (PMT-2), CE09015 (ACDH-1), CE12564 (R07H5.8), and CE09655 (RLA-0)], and three proteins were up-regulated [CE29645 (LEC-1), CE16576 (LEC-5), and CE01431 (NEX-1)]. RNAi phenotypes of two of the down-regulated genes, R07H5.8 (adenosine kinase) and rla-0 (ribosomal protein), in CN were similar to that of larval arrest in CS, and RNAi of a down-regulated gene, R07H5.8, in CS further enhanced the effects of CS, suggesting that downregulation of these genes is likely responsible for the larval arrest in CS. All three up-regulated genes contain putative DAF-16 binding sites and mRNA levels of these three genes were all decreased in daf-16 mutants in CN, suggesting that DAF-16 activates expression of these genes.

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Annexin I Is an Endogenous Ligand that Mediates Apoptotic Cell Engulfment

Cited by 382 publications

References 48 publications

Induction of annexin-1 during TRAIL-induced apoptosis in thyroid carcinoma cells

Induction of annexin-1 during TRAIL-induced apoptosis in thyroid carcinoma cells

Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor

Cholesterol-Responsive Metabolic Proteins Are Required for Larval Development in Caenorhabditis elegans

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