Annexin A5-Conjugated Quantum Dots with a Paramagnetic Lipidic Coating for the Multimodal Detection of Apoptotic Cells

Tilborg, Geralda A. F. van; Mulder, Willem J. M.; Chin, P.; Storm, Gert; Reutelingsperger, Chris; Nicolay, Klaas; Strijkers, Gustav J.

doi:10.1021/bc0600463

Cited by 143 publications

(115 citation statements)

References 11 publications

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“…11 Lipid-based nanoparticles, such as liposomes, micelles, and microemulsions, have been evaluated as carriers of MRI contrast and fluorescent agents for noninvasive imaging. 9,[12][13][14][15][16][17][18][19][20] There are several recent reviews on lipid-based paramagnetic nanoparticles 21,22 and on paramagnetic and fluorescent nanoparticles. 3,10,23,24 Kostarelos and Miller described a modular design for targeted paramagnetic liposomes carrying nucleic acids, termed Gd-ABCD.…”

Section: Introductionmentioning

confidence: 99%

Folate receptor-targeted fluorescent paramagnetic bimodal liposomes for tumor imaging

Ding¹,

J²,

Huang³

et al. 2011

IJN

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Rationale and objective: Receptor-targeted delivery of imaging and therapeutic agents can lead to enhanced efficacy for both. Multimodality imaging offers unique advantages over traditional single modality imaging. Tumor marker folate receptor (FR)-targeted fluorescent paramagnetic bimodal liposomes were synthesized to co-deliver paramagnetic and fluorescence agents for magnetic resonance (MR) and optical bimodal imaging contrast enhancement. Materials and methods: Fluorescent and paramagnetic bimodal liposomes were synthesized with a mean diameter of 136 nm and a low polydispersity index. The liposomes incorporated folate-PEG 3350 -CHEMS for FR targeting, Gd(III)[N,N-Bis-stearylamidomethyl-N'-amidomethyl]diethylenetriamine tetraacetic acid (Gd-DTPA-BSA) for MR contrast, and calcein for fluorescence. To determine the specificity and efficiency of delivery, the liposomes were evaluated in FR-positive KB and HeLa cells and FR-negative A549 cells, which were analyzed by fluorescence microscopy, magnetic resonance imaging (MRI), and flow cytometry (FCM). Results: FR-specific and efficient cellular uptake of the FR-targeted bimodal liposomes was confirmed by fluorescence microscopy and by FCM. The mean fluorescence intensity (MFI) of KB cells treated with FR-targeted liposomes was 45× that of cells treated with nontargeted liposomes, and 18× that of cells treated with FR-targeted liposomes and excess folic acid (FA). The MFI of HeLa cells treated with targeted liposomes was 33× that of nontargeted liposomes, and was 16× that of the mixture of targeted liposomes and free FA. In contrast, the MFI of A549 cells treated with FR-targeted liposomes was nearly the same as those treated with nontargeted liposomes. The T 1 -weighted MR images of HeLa and KB cells incubated with FR-targeted liposomes had much higher signal intensity than those treated with nontargeted liposomes or free Gd-DTPA. Furthermore, the FR-targeting effect could be blocked by excess free FA. Conclusion: FR-targeted fluorescent paramagnetic bimodal liposomes provided a novel platform for bimodal tumor imaging and theranostic delivery.

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Section: Introductionmentioning

confidence: 99%

Folate receptor-targeted fluorescent paramagnetic bimodal liposomes for tumor imaging

Ding¹,

J²,

Huang³

et al. 2011

IJN

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“…The fluorophores involved in these bioconjugates, however, were not QDs but fluorescein isothiocyanate (FITC), which might limit the applications for these bioconjugates because of the disadvantages of this organic dye. A hallmark of apoptosis is the externalization of phosphatidylserine, which is recognized by annexin V. Accordingly, van Tilborg et al (35 ) and Prinzen et al (36 ) used annexin V-conjugated QDs coated with a paramagnetic lipid to detect apoptotic cells with fluorescence and magnetic resonance imaging. We recently demonstrated that trifunctional avidin-coupled nanospheres are capable of recognizing apoptotic cells preincubated with annexin V-biotin via the strong biotin-avidin interaction (31 ).…”

mentioning

confidence: 99%

Visual Recognition and Efficient Isolation of Apoptotic Cells with Fluorescent-Magnetic-Biotargeting Multifunctional Nanospheres

et al. 2007

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Background: Fluorescent-magnetic-biotargeting multifunctional nanospheres are likely to find important applications in bioanalysis, biomedicine, and clinical diagnosis. We have been developing such multifunctional nanospheres for biomedical applications. Methods: We covalently coupled avidin onto the surfaces of fluorescent-magnetic bifunctional nanospheres to construct fluorescent-magnetic-biotargeting trifunctional nanospheres and analyzed the functionality and specificity of these trifunctional nanospheres for their ability to recognize and isolate apoptotic cells labeled with biotinylated annexin V, which recognizes phosphatidylserine exposed on the surfaces of apoptotic cells. Results:The multifunctional nanospheres can be used in combination with propidium iodide staining of nuclear DNA to identify cells at different phases of the apoptotic process. Furthermore, we demonstrate that apoptotic cells induced by exposure to ultraviolet light can be isolated simply with a magnet from living cells at an efficiency of at least 80%; these cells can then be easily visualized with a fluorescence microscope.

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“…Later also Gd-containing AnxV-coated liposomes for positive or bimodal MR contrast were developed [30,31] as well as multimodality contrast agents for combined MRI and fluorescent imaging [32].…”

Section: Annexin Vmentioning

confidence: 99%

Specific biomarkers of receptors, pathways of inhibition and targeted therapies: pre-clinical developments

Waerzeggers

Monfared

Viel

et al. 2011

BJR

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Annexin A5-Conjugated Quantum Dots with a Paramagnetic Lipidic Coating for the Multimodal Detection of Apoptotic Cells

Cited by 143 publications

References 11 publications

Folate receptor-targeted fluorescent paramagnetic bimodal liposomes for tumor imaging

Folate receptor-targeted fluorescent paramagnetic bimodal liposomes for tumor imaging

Visual Recognition and Efficient Isolation of Apoptotic Cells with Fluorescent-Magnetic-Biotargeting Multifunctional Nanospheres

Specific biomarkers of receptors, pathways of inhibition and targeted therapies: pre-clinical developments

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