Visual Recognition and Efficient Isolation of Apoptotic Cells with Fluorescent-Magnetic-Biotargeting Multifunctional Nanospheres

Song, Erqun; Wang, Guoping; Xie, Hai‐Yan; Zhang, Zhiling; Hu, Jun; Peng, Jun; Wu, Daocheng; Shi, Yun‐Bo; Pang, Dai‐Wen

doi:10.1373/clinchem.2007.092023

Cited by 63 publications

(41 citation statements)

References 38 publications

(28 reference statements)

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“…The g(r) represents the probability of finding a pair of beads at a distance r with respect to the bulk phase in a completely random distribution. It is defined as (13) where N AB is the total number of beads of A and B in the system, K is the number of time steps, δr is the distance interval, ΔN AB is the number of B (or A) beads between r and r + δr around an A (or a B) bead, and ρ AB is the bulk density. Note that A and B could be the same type of beads.…”

Section: Effects Of Ps Mws Of Ps−peo Blockmentioning

confidence: 99%

Dissipative Particle Dynamics Simulation on the Nanocomposite Delivery System of Quantum Dots and Poly(styrene-b-ethylene oxide) Copolymer

Qin

Lan

et al. 2015

Ind. Eng. Chem. Res.

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Dissipative particle dynamics (DPD) simulation was used to investigate the self-assembling dynamics process of poly(styrene-b-ethylene oxide) (PS-b-PEO) block copolymer and quantum dots (QDs) in an aqueous solution. The effects of molecular weight (MW) and segment construction of a PS−PEO block copolymer on the structure and size of the self-assembled micelles were discussed. The structural properties of micelles were characterized by a radial distribution function. The simulation results are qualitatively consistent with those of previous experiments and show that there are only small QD clusters. The hydrophobic PS chains form the micelle core, while the hydrophilic PEO chains form the shell. The size of the self-assembled PS−PEO/QDs micelle increases with the MW of PS-b-PEO block copolymer and the lengths of PEO and PS segments. The simulation results indicate that the assembling process includes four sequential transient stages: (1) the random distribution of all components in aqueous solution;(2) formation of small clusters with polymer chains and QDs; (3) crashing together of small spheres and the formation of larger aggregates; (4) stabilization of assembled micelles. The simulation reveals the physical insights of the QD loading mechanism of the PEG micelle at the mesoscopic scale, indicating the DPD simulation can be used as an adjunct to provide other valuable information for experiments.

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Section: Effects Of Ps Mws Of Ps−peo Blockmentioning

confidence: 99%

Dissipative Particle Dynamics Simulation on the Nanocomposite Delivery System of Quantum Dots and Poly(styrene-b-ethylene oxide) Copolymer

Qin

Lan

et al. 2015

Ind. Eng. Chem. Res.

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“…detection sensitivity due to the higher surface-tovolume ratio. [1,2] A nanosystem designed for bio-separation must satisfy the basic requirement of being able to accumulate promptly via a magnet (so that a pre-accumulation step is possible), while its size must be small enough to ensure a high sensitivity. Also, it must be easily dispersible in the medium in which it has to be exploited and to interact with cells or markers.…”

Section: Introductionmentioning

confidence: 99%

Magnetic–Fluorescent Colloidal Nanobeads: Preparation and Exploitation in Cell Separation Experiments

Corato

Piacenza

Musarò

et al. 2009

Macromolecular Bioscience

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Nanostructures displaying fluorescence and magnetic properties at the same time are potentially useful for achieving simultaneous bio-separation and bio-sensing (e.g., magnetic separation coupled with multiplexing optical detection of different tumour cell populations). Spherical nanobeads that display both fluorescent and magnetic features are reported; they are fabricated by grafting fluorescent oligothiophene molecules to an amphiphilic polymer that is then used to enwrap iron oxide nanoparticles, which acts as the magnetic domain. By tuning experimental conditions, control over the number of magnetic nanoparticles per bead and over the bead diameter (30-400 nm) was achieved. A cell separation efficiency of the level required for cell sorting applications is also reported.

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“…Quantum dots (QDs), 3 relatively new colloidal semiconductor nanocrystals, have been widely used as fluorescent probes in the fields of biomedical (1–5) and chemical-sensing and biosensing (6, 7) research owing to their stable and tunable multicolor fluorescence, broad absorption with narrow emission spectra, large molar extinction, high quantum yield, and high chemical stability (1, 2, 8). In addition, the fluorescence of different colors from QDs can be excited with a single laser source of energy above the band gap of most blue-emitting QDs, allowing versatile multicolored complex detection.…”

mentioning

confidence: 99%

Tumor Cell Targeting Using Folate-Conjugated Fluorescent Quantum Dots and Receptor-Mediated Endocytosis

et al. 2009

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BACKGROUND Luminescent nanobioprobes with cell-targeting specificity are likely to find important applications in bioanalysis, biomedicine, and clinical diagnosis. Quantum dots (QDs) are unique and promising materials for such a purpose because of their fluorescence and large surface area for attaching cell-targeting molecules. METHODS We produced water-dispersible QDs by coating hydrophobic QDs with small amphiphilic polyethylene glycol (PEG) molecules via hydrophobic interactions. We covalently coupled folate (FA) onto the water-dispersible PEG-coated QDs (PEG-QDs) to produce FA-coupled PEG-QDs (FA-PEG-QDs). RESULTS These FA-PEG-QD nanoparticles functioned as fluorescent nanobioprobes that specifically recognized folate receptors (FRs) overexpressed in human nasopharyngeal cells (KB cells) but not in an FR-deficient lung carcinoma cell line (A549 cells). Using confocal fluorescence microscopy, we demonstrated uptake of FA-PEG-QDs by KB cells but no uptake of folate-free PEG-QDs. The specificity of this receptor-mediated internalization was confirmed by comparing the uptake by KB vs A549 cells. CONCLUSIONS Our results suggest that such cell-targeting fluorescent nanobioprobes are potentially very powerful tools for recognizing target cells and delivering and tracking drugs and other therapeutic materials.

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Visual Recognition and Efficient Isolation of Apoptotic Cells with Fluorescent-Magnetic-Biotargeting Multifunctional Nanospheres

Cited by 63 publications

References 38 publications

Dissipative Particle Dynamics Simulation on the Nanocomposite Delivery System of Quantum Dots and Poly(styrene-b-ethylene oxide) Copolymer

Dissipative Particle Dynamics Simulation on the Nanocomposite Delivery System of Quantum Dots and Poly(styrene-b-ethylene oxide) Copolymer

Magnetic–Fluorescent Colloidal Nanobeads: Preparation and Exploitation in Cell Separation Experiments

Tumor Cell Targeting Using Folate-Conjugated Fluorescent Quantum Dots and Receptor-Mediated Endocytosis

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