ANLN and TLE2 in Muscle Invasive Bladder Cancer: A Functional and Clinical Evaluation Based on In Silico and In Vitro Data

Abstract

Anilin actin binding protein (ANLN) and transducing-like enhancer protein 2 (TLE2) are associated with cancer patient survival and progression. The impact of their gene expression on progression-free survival (PFS) of patients with muscle invasive bladder cancer (MIBC) treated with radical cystectomy (RC) and subtype association has not yet been investigated. qRT-PCR was used to measure the transcript levels of ANLN and TLE2 in the Mannheim cohort, and validated in silico by The Cancer Genome Atlas (TCGA) cohort. Uni-and multivariate Cox regression analyses identified predictors for disease-specific survival (DSS) and overall survival (OS). In the Mannheim cohort, tumors with high ANLN expression were associated with lower OS and DSS, while high TLE2 expression was associated with a favorable OS. The TCGA cohort confirmed that high ANLN and low TLE2 expression was associated with shorter OS and disease-free survival (DFS). In both cohorts, multivariate analyses showed ANLN and TLE2 expression as independent outcome predictors. Furthermore, ANLN was more highly expressed in cell lines and patients with the basal subtype, while TLE2 expression was higher in cell lines and patients with the luminal subtype. ANLN and TLE2 are promising biomarkers for individualized bladder cancer therapy including cancer subclassification and informed MIBC prognosis.

Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target

“…Expression of vimentin was found in 69% cases of transitional cell carcinoma and its expression is associated with the grade of transitional cell carcinoma [ 41 ]. In accordance with our study, Wu et al have also identified the expression of Anilin actin binding protein to be subtype-specific [ 42 ]. Similarly, the expression of Anilin actin binding protein was higher in the basal subtype compared to luminal subtype in bladder cancer cell lines.…”

Role of Anillin in Tumour: From a Prognostic Biomarker to a Novel Target

“…Transcriptome sequencing identified ANLN as a potential prognostic biomarker in bladder urothelial carcinoma [118]. Based on in silico and in vitro data, ANLN and TLE2 are shown to be potential biomarkers for muscle invasive bladder cancer [168]. ANLN was found as a target of head and neck squamous cell carcinomas via genome-wide gene expression profiling [169].…”

Prognostic Role of TLE Family in Lung Adenocarcinoma

“…High TLE2 expression was associated with favorable OS. The TCGA cohort confirmed that low TLE2 expression was associated with shorter OS and DFS [36].…”