“…Because the DNA sequences of isolated, recombinant antibodies are easily obtained, reagents can be expressed in Escherichia coli , and their affinity and specificity can be improved through directed mutagenesis to yield high‐quality, specific affinity reagents. By this approach, affinity reagents have successfully been generated to recognize a wide range of targets, including cell signaling proteins, membrane proteins, transcription factors, peptides, and post‐translational modifications (Gustafsson et al., ; Hornsby et al., ; Jones et al., ; Kim, Stroud, & Craik, ; Koide & Huang, ; Kummer et al., ; Pershad, Wypisniak, & Kay, ).…”