Anisotropic architecture and electrical stimulation enhance neuron cell behaviour on a tough graphene embedded PVA: alginate fibrous scaffold

Golafshan, Nasim; Kharaziha, Mahshid; Fathi, Mohammadhossein; Larson, Benjamin L.; Giatsidis, Giorgio; Masoumi, Nafiseh

doi:10.1039/c7ra13136d

Cited by 37 publications

(30 citation statements)

References 72 publications

(101 reference statements)

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“…However, incorporation of more α‐TCP nanopowders (>1 wt%) decreased mechanical properties attributing to the aggregation of nanopowder and inhomogeneous dispersion in PCL matrix. These nanopowders acted as stress concentrators and result in lower mechanical performance . Such these findings demonstrated in other researches …”

Section: Resultssupporting

confidence: 66%

Hemocompatible and Bioactive Heparin‐Loaded PCL‐α‐TCP Fibrous Membranes for Bone Tissue Engineering

Alehosseini

Golafshan

Kharaziha

et al. 2018

Macromolecular Bioscience

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The combination of bioactive components such as calcium phosphates and fibrous structures are encouraging niche-mimetic keys for restoring bone defects. However, the importance of hemocompatibility of the membranes is widely ignored. Heparin-loaded nanocomposite poly(ε-caprolactone) (PCL)-α-tricalcium phosphate (α-TCP) fibrous membranes are developed to provide bioactive and hemocompatible constructs for bone tissue engineering. Nanocomposite membranes are optimized based on bioactivity, mechanical properties, and cell interaction. Consequently, various concentrations of heparin molecules are loaded within nanocomposite fibrous membranes. In vitro heparin release profiles reveal a sustained release of heparin over the period of 14 days without an initial burst. Moreover, heparin encapsulation enhances mesenchymal stem cell (MSC) attachment and proliferation, depending on the heparin content. It is concluded that the incorporation of heparin within TCP-PCL fibrous membranes provides the most effective cellular interactions through synergistic physical and chemical cues.

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Section: Resultssupporting

confidence: 66%

Hemocompatible and Bioactive Heparin‐Loaded PCL‐α‐TCP Fibrous Membranes for Bone Tissue Engineering

Alehosseini

Golafshan

Kharaziha

et al. 2018

Macromolecular Bioscience

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“…PC‐12 cells could attach and grow well on the composite scaffold, and most importantly, moderate addition of GO significantly enhanced the differentiation toward neuron‐like cells in the presence of NGF, as confirmed by the upregulation of Tuj1 and GFAP expressions. Multilayer graphene doped hybrid alginate/PVA fibrous scaffolds with promoted both electrical and mechanical properties could also highly improve the initial adhesion and further spreading of PC‐12 cells . However, graphene/GO incorporated in fibrous scaffolds in that way are mostly encapsulated inside the fibers, which to a great extent hinders their interaction with neural cells.…”

Section: Graphene‐based Scaffolds For Neurogenesis and Myogenesismentioning

confidence: 99%

Electroactive Scaffolds for Neurogenesis and Myogenesis: Graphene‐Based Nanomaterials

Zhang

Klausen

Chen

et al. 2018

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One of the major issues in tissue engineering is constructing a functional scaffold to support cell growth and also provide proper synergistic guidance cues. Graphene‐based nanomaterials have emerged as biocompatible and electroactive scaffolds for neurogenesis and myogenesis, due to their excellent tunable chemical, physical, and mechanical properties. This review first assesses the recent investigations focusing on the fabrication and applications of graphene‐based nanomaterials for neurogenesis and myogenesis, in the form of either 2D films, 3D scaffolds, or composite architectures. Besides, because of their outstanding electrical properties, graphene family materials are particularly suitable for designing electroactive scaffolds that could provide proper electrical stimulation (i.e., electrical or photo stimuli) to promote the regeneration of excitable neurons and muscle cells. Therefore, the effects and mechanism of electrical and/or photo stimulations on neurogenesis and myogenesis are followed. Furthermore, studies on their biocompatibilities and toxicities especially to neural and muscle cells are evaluated. Finally, the future challenges and perspectives in facilitating the development of clinical translation of graphene‐family nanomaterials in treating neurodegenerative and muscle diseases are discussed.

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“…Graphene and graphene oxide-enriched scaffolds have been highlighted as promising candidates for neural regeneration applications (Ginestra 2019;Heidari et al 2019;Golafshan et al 2018;Zhao et al 2017;Aznar-Cervantes et al 2017;Qi et al 2019;Girão et al 2020;Magaz et al 2021). Graphene and graphene oxide are fascinating carbon-based materials that are preferred in the field of neuroscience applications due to their electrical conductivity and mechanical strength (Qi et al 2019;Magaz et al 2021).…”

Section: Nerve Tissue Engineering Scaffoldsmentioning

confidence: 99%

Electrospun Porous Biobased Polymer Mats for Biomedical Applications

Parın

Terzioğlu

2021

Advanced Functional Porous Materials

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The electrospinning method provides fabrication of nonwoven mats from nano to micrometers in size with controllable pores. Porous electrospun scaffolds have attracted increasing attention in biomedical applications especially due to their ability to mimic the extracellular matrix. This chapter presents a comprehensive overview of the advancements in the last five years through the design and preparation of porous biobased polymer mats using the electrospinning method for biomedical applications. Firstly, fundamentals of the electrospinning process are presented and followed by a discussion of the possible biomedical applications of micro-and nanostructured porous mats for drug delivery, wound dressing, tissue engineering, and other applications. Various crucial points for limitations and possible future trends are presented.

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Anisotropic architecture and electrical stimulation enhance neuron cell behaviour on a tough graphene embedded PVA: alginate fibrous scaffold

Abstract: Tough scaffolds comprised of aligned and conductive fibers are promising for peripheral nerve regeneration due to their unique mechanical and electrical properties.

Cited by 37 publications

References 72 publications

Hemocompatible and Bioactive Heparin‐Loaded PCL‐α‐TCP Fibrous Membranes for Bone Tissue Engineering

Hemocompatible and Bioactive Heparin‐Loaded PCL‐α‐TCP Fibrous Membranes for Bone Tissue Engineering

Electroactive Scaffolds for Neurogenesis and Myogenesis: Graphene‐Based Nanomaterials

Electrospun Porous Biobased Polymer Mats for Biomedical Applications

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