Anionic porphyrin as a new powerful cell death inducer of Tobacco Bright Yellow-2 cells

Riou, Catherine; Calliste, Claude-Alain; Silva, A. Da; Guillaumot, Damien; Rezazgui, Olivier; Sol, Vincent; Leroy‐Lhez, Stéphanie

doi:10.1039/c3pp50315a

Cited by 22 publications

(27 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ability of KL‐1, KL‐2, OL, AcKL‐1, AcKL‐2 and AcOL lignins to produce ROS was then investigated under dark and light exposure. Indeed, under light irradiation, so‐called photosensitizer compounds might undergo either a photoinduced electron transfer (Type I process) implying surrounding molecular oxygen or other substrate species, generating radicals such as the superoxide anion (O 2 •− ) and hydroxyl radical ( • OH), or energy transfer to dioxygen (Type II process), producing singlet oxygen ( 1 O 2 ) . To detect O 2 •− (type I process) and 1 O 2 (type II process), electron paramagnetic resonance (EPR) spectroscopy was used.…”

Section: Methodssupporting

confidence: 94%

Acetylated Lignins: A Potential Bio‐Sourced Photosensitizer

Marchand¹,

Calliste²,

Williams

et al. 2018

ChemistrySelect

View full text Add to dashboard Cite

Nowadays, lignins are receiving an increasing interest from the scientific community. Indeed, this biopolymer, formerly considered a waste‐product of the paper industry, appears today as an interesting oil equivalent in many fields. In this context, many chemical modifications of lignins have been presented to valorize this attractive feedstock. However, spectroscopic properties of these modified materials remain poorly studied, in particular their capacity to produce reactive oxygen species (ROS) under light irradiation. Thus, the purpose of this work is to monitor the production of singlet oxygen and superoxide anion under light irradiation of three different acetylated lignins. The results obtained show that those modified lignins can generate ROS levels, allowing to consider a use of these materials as photosensitizers for applications such as photodynamic treatments.

show abstract

Section: Methodssupporting

confidence: 94%

Acetylated Lignins: A Potential Bio‐Sourced Photosensitizer

Marchand¹,

Calliste²,

Williams

et al. 2018

ChemistrySelect

View full text Add to dashboard Cite

show abstract

“…The reason for this decrease may be the generation of ROS, which overwhelms the antioxidant defenses. HpD induced oxidative stress, which led to the generation of free radicals and alterations in antioxidants enzymes in plant cells (Riou et al 2014). Porphyrin inactivated catalase through the production of singlet oxygen, which caused amino acid damage (Hirakawa et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Photosensitizing Effects of Hematoporphyrin Dihydrochloride against the Flesh FlyParasarcophaga argyrostoma(Diptera: Sarcophagidae)

Abdelsalam

Korayem

El-Sherbini³

et al. 2014

Florida Entomologist

View full text Add to dashboard Cite

“…All these ROS damage cellular constituents such as nucleic acids, proteins, lipids leading to cell death by necrosis and more often by apoptosis (Gill and Tuteja , Avery ). As a consequence of porphyrin photoactivation, cell death was shown in animal cells, fungi, bacteria and recently in tobacco BY‐2 cells (Donnelly et al , Ringot et al , Pläetzer et al , Riou et al , Maish ). Because lipid peroxidation is thought to be mainly triggered by these ROS, we performed MDA quantification on Arabidopsis whole plantlets (Gill and Tuteja , Avery ).…”

Section: Discussionmentioning

confidence: 99%

“…All porphyrins were soluble in water and their chemical structures were reported (Riou et al ). Briefly, anionic porphyrin (AP and AP‐Zn) correspond to the free zinc sulfonated tetraphenylporphyrin (H 2 TPPS) and Zinc‐TPPS, respectively.…”

Section: Methodsmentioning

confidence: 99%

Responses of an adventitious fast‐growing plant to photodynamic stress: comparative study of anionic and cationic porphyrin effect on Arabidopsis thaliana

Issawi

Guillaumot

Sol

et al. 2017

Physiologia Plantarum

View full text Add to dashboard Cite

Antimicrobial photodynamic treatment (APDT) based on the use of a photosensitizer to produce reactive oxygen species (ROS) that induce cell death could be envisaged to fight against plant pathogens. For setting this strategy, we want to study how plants themselves respond to photodynamic treatment. In previous work we showed that tomato plantlets were able to resist photoactivated tetra (N-methylpyridyl) porphyrin (CP) or the zinc metalated form (CP-Zn). To enlarge our plant expertise related to exogenous porphyrins treatment and to further defend this approach, we studied how a weed like Arabidopsis thaliana responded to exogenous supply of anionic and cationic porphyrins. Both types of photosensitizers had no negative effect on seed germination and did not hamper the development etiolated Arabidopsis plantlet under dark conditions. Thus, post-emergence effects of porphyrin photoactivation on the development of 14 day-old in vitro Arabidopsis plantlet under light were observed. CP-Zn was the most efficient photosensitizer to kill Arabidopsis plantlets while anionic tetra (4-sulfonatophenyl) porphyrin only delayed their growth and development. Indeed only 7% of plantlets could be rescued after CP-Zn treatment. Furthermore, non-enzymatic and enzymatic defense components involved in detoxification of ROS generated by CP-Zn under illumination were downregulated or stable with the exception of sevenfold increase in proline content. As previously demonstrated in the literature for microbial agents and in the present work for Arabidopsis, CP-Zn was efficient enough to eradicate unwanted vegetation and plant pathogens without at the same time killing plants of agronomic interest such as tomato plantlets.

show abstract

Anionic porphyrin as a new powerful cell death inducer of Tobacco Bright Yellow-2 cells

Cited by 22 publications

References 30 publications

Acetylated Lignins: A Potential Bio‐Sourced Photosensitizer

Acetylated Lignins: A Potential Bio‐Sourced Photosensitizer

Photosensitizing Effects of Hematoporphyrin Dihydrochloride against the Flesh FlyParasarcophaga argyrostoma(Diptera: Sarcophagidae)

Responses of an adventitious fast‐growing plant to photodynamic stress: comparative study of anionic and cationic porphyrin effect on Arabidopsis thaliana

Contact Info

Product

Resources

About