Anionic phospholipid coatings in capillary electrochromatography

Hautala, Jari T.; Riekkola, Marja‐Liisa; Wiedmer, Susanne Κ.

doi:10.1016/j.chroma.2006.08.025

Cited by 22 publications

(13 citation statements)

References 38 publications

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“…The mean particle sizes were also found to increase, whereas the mean zeta potential decreased, when the concentration of calcium ions was increased, with the exception of the sample prepared with 0.100 M calcium ions. The negative charge of the DOPA liposome template was suppressed upon increasing of the concentration of calcium ions (Hautala et al, 2007). Thus, the zeta potential changed from strongly negative to weakly negative.…”

Section: Polydispersity Indexmentioning

confidence: 81%

“…Thus, the zeta potential changed from strongly negative to weakly negative. However, the most significant change in the zeta potential value was observed in sample C-DOPA-4 (0.100 M NaOH and 0.100 M calcium ions), presumably due to the strong interactions between the calcium ions and the DOPA template, which are uniform in every direction in this sample (Hautala et al, 2007). Thus, the binding of calcium ions is a specificity factor for the change in the zeta potential.…”

Section: Polydispersity Indexmentioning

confidence: 94%

“…Moreover, the liposome (template), which contains phosphatidic acid, possesses an especially strong affinity for calcium ions. Calcium ions exhibit the ability to bind to phosphate groups of the DOPA template, which reduces the relative amount of free, negatively charged phosphate groups (Hautala et al, 2007). Thus, the zeta potential changed from strongly negative to weakly negative.…”

Section: Polydispersity Indexmentioning

confidence: 99%

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Investigation into the role of NaOH and calcium ions in the synthesis of calcium phosphate nanoshells

Yeo

Zein

Ahmad

et al. 2012

Braz. J. Chem. Eng.

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-Calcium phosphate (CaP) nanoshells were prepared using negatively charged liposomes (1,2-dioleoyl-sn-glycero-3-phosphate sodium salt (DOPA)) as a template by base titration synthesis at various concentrations of NaOH and calcium ions. The elemental composition, morphology, particle size, particle size distribution and zeta potential of the products were determined via various characterisation techniques, such as energy-dispersive X-ray spectrometry (EDX), transmission electron microscopy (TEM), dynamic light scattering (DLS), laser Doppler velocimetry (LDV) and Fourier transform infrared spectroscopy (FTIR). The best results showed that stable spherical CaP nanoshells with a mean particle size of 197.5 ± 5.8 nm and a zeta potential of -34.5 ± 0.6 mV were successfully formed when 0.100 M sodium hydroxide (NaOH) and 0.100 M calcium ions were used. Moreover, an optimal pH of 10.52 and a final Ca/P molar ratio of 0.97 were achieved under these conditions.

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Section: Polydispersity Indexmentioning

confidence: 81%

Section: Polydispersity Indexmentioning

confidence: 94%

Section: Polydispersity Indexmentioning

confidence: 99%

See 1 more Smart Citation

Investigation into the role of NaOH and calcium ions in the synthesis of calcium phosphate nanoshells

Yeo

Zein

Ahmad

et al. 2012

Braz. J. Chem. Eng.

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“…The Z ‐average size was 294 nm (PDI of 0.18) and 282 nm (PDI 0.09) for Intralipid® and ClinOleic®, respectively. The size of both Intralipid® and ClinOleic® is bigger and the polydispersity higher when compared to extruded POPC/POPG vesicles, with the size of 132 nm and PDI of 0.04 [].…”

Section: Resultsmentioning

confidence: 99%

Comparison of lipid sinks in sequestering common intoxicating drugs

Lokajová

Holopainen

Wiedmer

2012

J of Separation Science

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Intravenous lipid emulsion is a recommended treatment for local anesthetic intoxication. The lipid sink theory hypothesizes that the mechanism behind the lipid treatment is the entrapment of toxic drugs in plasma, preventing them from reaching target receptors. Lipid sink treatment has also been used as a last refuge treatment for severe tricyclic antidepressant intoxication with seemingly beneficial results. We selected three drugs, i.e. amiodarone, ketamine, and amitriptyline, that can cause severe intoxication and compared their interactions with two commercial fat emulsions (Intralipid® and ClinOleic®) and one synthetic liposome (80:20 mol% phosphatidylcholine/phosphatidylglycerol) dispersion. The interaction studies were carried out by capillary electrokinetic chromatography and the retention factors and distributions constants of the drugs were calculated. The results demonstrate that there is stronger interaction between the drugs and the synthetic liposome dispersion than with the commercial emulsions.

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“…Hautala et al coated the fused silica capillaries with anionic liposomes comprising POPC and PS and/or cholesterol (all phospholipids in fluid state) [58][59][60][61]. Cunliffe et al [62] used calcium in the coating of fused silica capillaries with liposomes composed of 1,2-dilauroyl-sn-phosphatidylcholine (DLPC), and they found that, without Ca 2+ , the coating time was prolonged almost 20-fold.…”

Section: Liposome Capillary Electrochromatography (Lcec)mentioning

confidence: 99%

Profiling Drug Membrane Permeability and Activity Via Biopartitioning Chromatography

Sun¹,

Wu²,

Lu³

et al. 2008

CDM

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Drug in vivo pharmacokinetic performances in nature consist of sequential membrane transporting processes and are based on the entry into and exit of drugs from cell, even for metabolism process requiring parent drugs delivered into and metabolites effluxed from the metabolizing cells. Efficient and reliable high throughput screen of membrane permeability properties as early as possible in drug discovery and development program is accordingly desirable. Biopartitioning chromatography (BPC) introduces biomembrane-mimetic structures (such as liposome, phospholipid monolayer, micelle, microemulsion, vesicle and bicelle, etc) into chromatographic system, i.e. liquid system or capillary electrophoresis, and thereby emulates drug-membrane interactions difficult to study in the liquid state by well reproducible, rapid, sensitive and adequately designed chromatographic technique. And recently BPC has been becoming a high-throughput screening platform for drug membrane permeability and biological activity. The theoretical basis, classification and application of BPC were summarized based on the latest advances and our recent works. The development potential and perspectives of this field were also discussed.

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Anionic phospholipid coatings in capillary electrochromatography

Cited by 22 publications

References 38 publications

Investigation into the role of NaOH and calcium ions in the synthesis of calcium phosphate nanoshells

Investigation into the role of NaOH and calcium ions in the synthesis of calcium phosphate nanoshells

Comparison of lipid sinks in sequestering common intoxicating drugs

Profiling Drug Membrane Permeability and Activity Via Biopartitioning Chromatography

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