Profiling Drug Membrane Permeability and Activity Via Biopartitioning Chromatography

Sun, Jin; Wu, Xin; Lu, Rong; Liu, Jianfang; Wang, Yongjun; Zhang, He

doi:10.2174/138920008783571800

Cited by 22 publications

(6 citation statements)

References 79 publications

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“…liposomes, phospholipid monolayers, micelles, or bicelles) is introduced into a chromatographic system to study drug-membrane interactions [228]. Pure and highly stable phases of PEG-stabilized bilayer disks (described in Section 2.4) were developed as model membranes for drug partition studies, and have the potential to give more accurate results than liposomes [229].…”

Section: Interaction Of Small Molecules With Bicellesmentioning

confidence: 99%

When detergent meets bilayer: Birth and coming of age of lipid bicelles

Dürr

Soong

Ramamoorthy

2013

Progress in Nuclear Magnetic Resonance Spectroscopy

116

108

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Section: Interaction Of Small Molecules With Bicellesmentioning

confidence: 99%

When detergent meets bilayer: Birth and coming of age of lipid bicelles

Dürr

Soong

Ramamoorthy

2013

Progress in Nuclear Magnetic Resonance Spectroscopy

116

108

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“…In the last few years BPC has become a high-throughput screening platform to study drug-membrane interaction and permeability and their correlation with the biological effects. [55]…”

Section: Analytical Techniques Available To Study Drug-membrane Modelmentioning

confidence: 99%

Biomembrane models and drug-biomembrane interaction studies: Involvement in drug design and development

et al. 2011

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Contact with many different biological membranes goes along the destiny of a drug after its systemic administration. From the circulating macrophage cells to the vessel endothelium, to more complex absorption barriers, the interaction of a biomolecule with these membranes largely affects its rate and time of biodistribution in the body and at the target sites. Therefore, investigating the phenomena occurring on the cell membranes, as well as their different interaction with drugs in the physiological or pathological conditions, is important to exploit the molecular basis of many diseases and to identify new potential therapeutic strategies. Of course, the complexity of the structure and functions of biological and cell membranes, has pushed researchers toward the proposition and validation of simpler two- and three-dimensional membrane models, whose utility and drawbacks will be discussed. This review also describes the analytical methods used to look at the interactions among bioactive compounds with biological membrane models, with a particular accent on the calorimetric techniques. These studies can be considered as a powerful tool for medicinal chemistry and pharmaceutical technology, in the steps of designing new drugs and optimizing the activity and safety profile of compounds already used in the therapy.

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“…This HPLC column has been designed for the prediction of drug-membrane interactions. There is evidence that the retention measured on this column correlates to traditional in-vitro methods such as Caco-2 cell line cultures, intestinal tissue, or liposome assays [34,35,36]. However, it has been previously discovered that inorganic anions are also able to retain on a phospholipid monolayer by Hu et al [37].…”

Section: Introductionmentioning

confidence: 99%

Levels of the Thiocyanate in the Saliva of Tobacco Smokers in Comparison to e-Cigarette Smokers and Nonsmokers Measured by HPLC on a Phosphatidylcholine Column

Flieger

Kawka

Tatarczak-Michalewska

2019

Molecules

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The aim of the study was to estimate the thiocyanate levels in saliva of cigarette smokers in comparison to e-cigarette smokers and nonsmokers. To improve our understanding of the influence of smoking on the oral level of thiocyanate, we conducted an assessment of human saliva, in 24 individuals (eight tobacco smokers, eight e-cigarette smokers, and eight nonsmokers). High-Performance Liquid Chromatography with ultraviolet detection (HPLC-UV) using a unique phosphatidylcholine column was applied in this assay. Thiocyanate ion was detected directly by its absorbance at 210 nm. The method presents a new application of the IAM (Immobilized Artificial Membrane) column for quantification of inorganic anions. The whole process meets the criteria of green chemistry because it was carried out without the use of organic solvents. For compensating matrix effects, an eight-point standard addition protocol was used to quantify the thiocyanate level in saliva samples. The calibration graphs were linear in the range of 5–100 mg L−1 with a correlation coefficient higher than 0.99. The thiocyanate concentrations in the saliva of tobacco smokers, e-cigarette smokers, and nonsmokers were found in the range of 121.25–187.54 mg L−1, 121.24–244.11 mg L−1, 33.03–79.49 mg L−1, respectively. The present study indicates an obvious statistically significant elevation in salivary thiocyanate level in tobacco smokers in comparison to nonsmokers. The phosphatidylcholine-based stationary phase proved to be suitable for the detection and quantification of the thiocyanate ion. The salivary thiocyanate levels in e-cigarette smokers were not significantly different in comparison to tobacco smokers but higher if compared to nonsmokers. The criterion for statistical significance was p < 0.05.

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Profiling Drug Membrane Permeability and Activity Via Biopartitioning Chromatography

Cited by 22 publications

References 79 publications

When detergent meets bilayer: Birth and coming of age of lipid bicelles

When detergent meets bilayer: Birth and coming of age of lipid bicelles

Biomembrane models and drug-biomembrane interaction studies: Involvement in drug design and development

Levels of the Thiocyanate in the Saliva of Tobacco Smokers in Comparison to e-Cigarette Smokers and Nonsmokers Measured by HPLC on a Phosphatidylcholine Column

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